Raymond J. Kelleher PhD

Raymond Kelleher

Raymond J. Kelleher
PhD

Research Associate Professor

Department of Microbiology and Immunology

Jacobs School of Medicine & Biomedical Sciences


Specialty/Research Focus

Apoptosis and cell death; Autoimmunity; Bioinformatics; Cancer Treatment and Prevention; Cell Cycle; Clinical and Laboratory Immunology-Allergy and Immunology; Endocrinology; Gene Expression; Gene therapy; Genomics and proteomics; Image Analysis; Immunoassays; Immunogenicity; Immunology; Molecular and Cellular Biology; Molecular genetics; Regulation of metabolism; Signal Transduction; Transcription and Translation

Contact Information
Jacobs School of Medicine and Biomedical Sciences
955 MainStreet
Room 5122
Buffalo, New York 14203
Phone: (716) 829-2558
rjk6@buffalo.edu



Professional Summary:

I am the administrator for flow cytometry in the Confocal Microscopy and Flow Cytometry Core Facility that serves investigators throughout the university. In that role, I oversee the use of the LSRFortessa and the FACSCelesta analytical flow cytometers, providing instruction on their use and the analysis of acquired data and serving as a consultant on the design and interpretation of experiments. I also operate the FACSFusion cell sorter, providing sterile live cell sorting. In addition, I operate and provide assistance to users on the application of cytometric bead array analysis on the FACSArray, as well as Elispot analysis using the Zeiss KS-ELISPOT microscope.

My own research centers on investigating the responsiveness of human T cells in the tumor microenvironment of lung and ovarian cancer and lymphomas. In that research, I am a coinvestigator in a collaborative group of oncologists and immunologists coordinated by Richard Bankert, PhD. We have observed that T cells in the tumor microenvironment are hyporesponsive to T cell receptor-mediated activation and that factor(s) present in ovarian tumors and associated ascites fluid can cause this hyporesponsiveness. We are investigating the mechanism(s) of this phenomenon. Also, as an approach to the in vivo study of the immune response to human tumor associated antigens, our group has established a novel xenograft model by injecting human tumor cell aggregates of solid ovarian tumor biopsies intraperitoneally into immune-deficient NSG mice. The result is a human tumor microenvironment in the greater omentum of the mice, i.e., the omental tumor xenograft (OTX) model. The progression of the human tumor xenograft closely approximates the characteristics of the tumor in cancer patients, and it is possible to quantify the presence of tumor cells and stromal cells in the OTX model.

These findings have led to our program goals to: 1.) determine whether the OTX model can be used as a predictive tool of the outcome of therapeutic approaches for the treatment of human ovarian cancer and B cell lymphoma, and 2.) determine whether the inhibition of activation in the tumor microenvironment can be reversed so that the antitumor T cell response can be reactivated.

I am also a member of the Institutional Animal Care and Use Committee (IACUC).

Education and Training:

  • Fellowship, Biochemistry and Immunology, The Salk Institute for Biological Studies (1972)
  • PhD, Genetics, University of North Carolina, Chapel Hill (1969)
  • MS, Biology, Boston College (1964)
  • BA, Biology, College of the Holy Cross (1961)

Employment:

  • Research Associate Professor, Microbiology and Immunology, University at Buffalo Jacobs School of Medicine & Biomedical Sciences (2010-present)
  • Research Assistant Professor, Department of Microbiology & Immunology, University at Buffalo Jacobs School of Medicine & Biomedical Sciences (2002–2010)
  • Cancer Research Scentist, Roswell Park Cancer Institute (1998–2003)
  • Visiting Scientist, Roswell Park Cancer Institute (1996–1998)
  • Senior Research Scientist, Lederle-Praxis Biologicals (1993–1996)
  • Research Affiliate, Roswell Park Cancer Institute (1992–1993)
  • Vice President for Research, T & B Bioclone, Inc. (1987–1992)
  • Adjunct Assistant Professor, SUNY at Buffalo Department of Biological Sciences (1984–1990)
  • Assistant Professor, Department of Biology, SUNY at Geneseo (1984–1987)
  • Assistant Professor, Department of Biological Sciences, SUNY at Buffalo (1977–1984)
  • Senior Research Associate, The Salk Institute, LaJoLLA, CA (1972–1977)
See all (1 more)

Awards and Honors:

  • Associate Member, Witebsky Center for Microbial Pathogenesis & Immunology (2004)
  • Listed in "American Men and Women in Science". (1982)
  • University Awards Committee, Faculty Research Fellowship, State University of New York (1980)
  • Travel Grant from Genetics Society of America for XIVth International Congress of Genetics; Moscow, USSR (1978)
  • Post-Baccalareate Fellowship (1969)
  • Elected to Full Membership, Society of the Sigma Xi (1966)
  • Pre-baccalaureate fellowship (1964)
  • Full Academic Scholarship (1957)

Research Expertise:

  • Biochemistry
  • Flow Cytometry
  • Genetics
  • Immunobiology
  • Molecular Biology

Research Centers:

  • Witebsky Center for Microbial Pathogenesis and Immunology

UB 2020 Strategic Strengths:

  • Molecular Recognition in Biological Systems and Bioinformatics

Grants and Sponsored Research:

  • December 2007–June 2020
    “Re-activating Memory T Cells in the Microenvironment of Human Tumors”
    NIH/NCI
    Role: Co-Investigator

Journal Articles:

See all (18 more)

Service Activities:

  • Institutional Animal Care and Use Committee (2019–present)
  • Proceedings of the National Academy of Sciences, Somatic Cell Genetics, Cancer Immunology and Immunotherapy, Immunological Investigations, Journal of Hematoloy, Cytokine (2015–2021)
  • Buffalo Philharmonic Chorus; Singing Member (Tenor), Board Member, Chair of Finance Committee (2002–present)
  • Contributed questions to Educational Testing Service for inclusion in Biology Advanced Graduate Record Examination (1995–2010)

School News:

In the Media:


Clinical Specialties:

Clinical Offices:

Insurance Accepted:



Contact Information

Jacobs School of Medicine and Biomedical Sciences
955 MainStreet
Room 5122
Buffalo, New York 14203
Phone: (716) 829-2558
rjk6@buffalo.edu