Alice L. Crane.

Alice L. Crane

Department

Pharmacology and Toxicology

Thesis

The Impact of Genetic Variability on Human Susceptibility to Chlorpyrifos (Completed 2014)

We look at biomarkers of effect, exposure, and susceptibility in relation to Chlorpyrifos and other organophosphorus pesticide exposure. We use both in vitro and in vivo techniques and currently have access to a cohort of Egyptian pesticide workers in order to study chronic exposure to these pesticides.

Residency Placement

Surgery Prelim, Cleveland Clinic
Urology, Cleveland Clinic

Undergraduate Education

Biology (Molecular) and Classics, University of Pennsylvania

Thesis Advisor

Publications/Abstracts Presented

  1. Farahat, F., Ellison, C., Bonner, M., McGarrigle, B., Crane, A., Fenske, R., Lasarev, M., Rohlman, D., Anger, W., Lein, P., and Olson, J. Biomarkers of Chlorpyrifos exposure and effect in Egyptian cotton field workers. 2011. Environmental Health Perspectives.
  2. Crane, A.L., Klein, K., Zanger, U.M., and Olson, J.R. Effect of CYP2B6*6 and CYP2C19*2 Genotype on Chlorpyrifos Metabolism. 2012. Toxicology.
  3. Crane, A.L., Klein, K., and Olson, J.R. Bioactivation of Chlorpyrifos by CYP2B6 Variants. 2012. Xenobiotica.
  4. Ellison*, C.A., Crane*, A.L.,  Bonner, M.R., Knaak, J.B., Browne, R.W., Lein, P.J., and Olson, J.R. Paraoxonase 1 Status in Egyptian Agricultural Workers Exposed to the Organophosphorus Pesticide Chlorpyrifos. 2012. Toxicology and Applied Pharmacology.
    *Equal contributing first authors
  5. Crane, A.L., Singleton, S.T., Laserev, M. R., Ismail, A., Abdel Rasoul, G., Hendy, O., McGarrigle, B.P., Bonner, M.R., Olson, J.R., and Rohlman, D.S. Longitudinal Assessment of Chlorpyrifos Exposure and Effect Biomarkers in Adolescent Egyptian Agricultural Workers. 2012. Journal of Exposure Science and Environmental Epidemiology.

Book Chapters

Ellison*, C.A., Crane*, A.L., Olson, J.R. Biotransformation of Insecticides. In Metabolism of Drugs and other Xenobiotics (Editors: Anzenbacher, P. and Zanger, U.M.), Wiley/Verlag Chemie Weinheim. 2012.
*Equal contributing first authors

Abstracts

  1. Crane, A., Browne, R., Knaak, J., Bonner, M., Fenske, R., Farahat, F., Anger, W.K., Lein,P., and Olson, J. 2010. Inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in human blood following in vitro and in vivo exposure to chlorpyrifos. Abstract for poster presentation, Society of Toxicology, Salt Lake City, UT. 2010 and Pharmacology Day, Buffalo, NY. 2010.
  2. Crane, A., McGarrigle, B., and Olson, J. 2011. Metabolism of Chlorpyrifos by Allelic Isoforms of CYP2B6, a Key Bioactivating Enzyme. Abstract for poster presentation, Society of Toxicology, Washington D.C. 2011.
  3. Hussainzada, N., Jackson, D., Bruun, D., Milatovic, D., Lewis, J., Banks, C., Crane, A., Singleton, S., Aschner, M., Browne, R.W., Olson, J.R., and Lein, P. Biomarkers of Neurotoxicity in a Rat Model of Occupational Chlorpyrifos (CPF) Exposure. Abstract for poster presentation. Society of Toxicology, Washington D.C. 2011.
  4. Crane, A. and Olson, J. Effect of CYP2B6*6 and CYP2C19*2 Genotype on Chlorpyrifos Metabolism: Implications for Human Risk. Abstract for poster presentation, University at Buffalo (SUNY) Department of Pharmacology and Toxicology Mini Research Symposium, 2011.
  5. Rohlman, D.S., Abdel Rasoul, G., Ismail, A., Olson, J.R., Crane, A.L., and Hendy, O. Occupational Pesticide Exposure in Egyptian Adolescents. Abstract for presentation at Global Health Conference, Montreal, Canada, 2011.
  6. Crane, A.L.,  Klein, K., Zanger, U.M., and Olson, J.O. Effect of CYP2B6 Variants on Chlorpyrifos Metabolism: Implications for Human Risk. Abstract for poster presentation, Society of Toxicology, San Francisco, CA, 2012.
  7. Singleton, S.T., Crane, A.L., Ismail, A., Abdel Rasoul, G., Hendy, O., McGariggle, B.P., Olson, J.R., and Rohlman, D. S. Chlorpyrifos Exposure in Adolescent Egyptian Agricultural Workers. Abstract for poster presentation, Society of Toxicology, San Francisco, CA, 2012.
  8. Ellison, C.A., Crane, A.L.,  Bonner, M.R., Knaak, J.B., Browne, R.W., Lein, P.J., and Olson, J.R. Paraoxonase 1 Status in Egyptian Agricultural Workers Exposed to the Organophosphorus Pesticide Chlorpyrifos. Abstract for poster presentation, Society of Toxicology, San Francisco, CA, 2012.

Professional/Educational Meetings Attended

  • Society of Toxicology Conference. Baltimore, MD. March 2009.
  • Society of Toxicology Conference. Salt Lake City, UT. 2010.

Awards/Honors

  • Dean’s Commendation, Year 1 & 2
  • 2nd place graduate student poster award, Pharmacology Day, 2010

Medical School Activities

  • Professional Conduct Committee
  • Siegel Award Committee
  • Pharmacology and Toxicology GSA club (President)

Extracurricular Interests

  • Music (classical and jazz trumpet, drums)
  • Krav Maga