Jason Rizzo.

Jason Rizzo

Department

Biochemistry

Thesis

Resolving genome-wide views of chromatin structure to identify fundamental principles of genome organization (Completed 2012)

Human genetic information is encoded in DNA sequences and packaged precisely by molecules known as nucleosomes. Nucleosomes are found in all eukayotic organisms and inhibit key intracellular interactions that regulate how genetic information is read, including transcription factor binding to DNA. Our lab employs both computational and experimental approaches to study how chromatin structure influences gene regulation in normal and disease states. We utilize emerging high-throughput DNA sequencing technologies (e.g. ChIP-seq, MNase-seq, FAIRE-seq) and novel methods in computational biology to explore the relationship between chromatin structure & regulatory factor targeting on a genome-wide scale. Our experimental systems range from cell culture & eukaryotic models like S. cerevisiae to patient tumor samples and pathogenic fungi such as C. albicans.

Residency Placement

Dermatology, University of Michigan Hospitals, Ann Arbor, MI.

Undergraduate Education

Biochemistry, Syracuse University

Thesis Advisor

Publications/Abstracts Presented

  • Rizzo JM, Loss LC, Rothman IL. The Clinical Picture: Hyperpigmented Patches on the Neck, Shoulder, and Back. Cleve Clin J Med. 2013 May;80(5):290-296.
  • Rizzo JM and Buck MJ. Key Principles and Clinical Applications of "Next-Generation" DNA Sequencing. Cancer Prev Res (Phila). 2012 Jul;5(7):887-900.
  • Givens RM, Lai WK, Rizzo JM, Bard JE, Mieczkowski PA, Leatherwood J, Huberman JA, Buck MJ. Chromatin architectures at fission yeast transcriptional promoters and replication origins. Nucleic Acids Res. 2012 Aug 1;40(15):7176-89.
  • Rizzo JM, Bard JE, Buck MJ. Standardized collection of MNase-seq experiments enables unbiased dataset comparisons. BMC Mol Biol. 2012 May 6;13(1):15. Highly Accessed.
  • Li SC, Diakov TT, Rizzo JM, Kane PM. Vacuolar H+-ATPase works in parallel with the HOG pathway to adapt Saccharomyces cerevisiae cells to osmotic stress. Eukaryot Cell. 2012 Mar;11(3):282-91.
  • Rizzo JM, Mieczkowski PA, Buck MJ. Tup1 stabilizes promoter nucleosome positioning and occupancy at transcriptionally plastic genes. Nucleic Acids Res. 2011 Nov 1;39(20):8803-19.
  • Hanlon SE, Rizzo JM, Tatomer DC, Lieb JD, Buck MJ. The stress response factors Yap6, Cin5, Phd1, and Skn7 direct targeting of the conserved co-repressor Tup1-Ssn6 in S. cerevisiae. PLoS One. 2011 Apr 28;6(4):e19060.
  • Rizzo JM and Buck MJ. All Things ChIP: ChIP-Chip, ChIP-Seq, ChIP-PCR. Encyclopedia of Molecular Cell Biology and Molecular Medicine. 2011 ISBN: 9783527600908.
  • Rizzo JM, Tarsio M, Martínez-Muñoz GA, Kane PM. Diploids heterozygous for a vma13Delta mutation in Saccharomyces cerevisiae highlight the importance of V-ATPase subunit balance in supporting vacuolar acidification and silencing cytosolic V1-ATPase activity. J Biol Chem. 2007 Mar 16;282(11):8521-32.

Professional/Educational Meetings Attended

  • An Immunohistochemical and RT-PCR Evaluation of Dermatofibrosarcoma Protuberans (DFSP) for Platelet-derived Growth Factor Beta (PDGFB) and Platelet-derived Growth Factor Receptor Beta (PDGFRB). Mohs College Annual Meeting. Fairmont Hotel, Chicago, IL.May 3rd-6th, 2012. Poster session.
  • Evaluating the roles of chromatin structure and Tup1p in determining C. albicans virulence using next-generation DNA sequencing. AAP/ASCI joint meeting. Fairmont Hotel, Chicago, IL. April 27-29th, 2012. Poster session.
  • Evaluating the roles of chromatin structure and Tup1p in determining C. albicans virulence using next-generation DNA sequencing. CSCR/MWAFMR joint meeting. Marriot Hotel, Chicago, IL.April 26-27th, 2012. Poster session.
  • Artifact-free MNase-seq Comparisons Epigenetics of Cancer and Stem Cells Symposium.University of Toronto. February 27th, 2012. Poster session.
  • Tup1 stabilizes promoter nucleosome positioning and occupancy at transcriptionally plastic genes. Northeast Regional Yeast Meeting (NERY), University of Rochester. October 14th -15th, 2011. Poster session.
  • Tup1 stabilizes promoter nucleosome positioning and occupancy at transcriptionally plastic genes. 9th Annual Biochemistry Research Day, SUNY at Buffalo. May 9th, 2011. Oral presentation.           
  • An improved chromatin mapping approach allows genome-wide chromatin structure comparisonsusing next-generation DNA sequencing. AAP/ASCI joint meeting. Fairmont Hotel, Chicago, IL. April 15-17th, 2011. Poster session.
  • Matched MNase digests Reveal Tup1 Nucleosome Stabilization Genome-wide.  In Silico Methodsand High-throughput Screening UB2020 Symposium, SUNY at Buffalo. September 11th, 2010. Poster session.
  • Matched MNase digests: a more accurate and reproducible method for assaying chromatin structure. Northeast Regional Yeast Meeting (NERY), SUNY at Buffalo. June 9th, 2010. Oral presentation.
  • Genome-wide views of chromatin structure. 8th Annual Biochemistry Research Day, SUNY atBuffalo. April 30th, 2010. Oral presentation.
  • Modeling Nucleosome Inhibition of Transcription Factor Binding. 7th Annual BiochemistryResearch Day, SUNY at Buffalo. May 9th, 2009. Poster session.
  • Management of Dermatofibrosarcoma protuberans. 21st World Congress of Dermatology, BuenosAires, Argentina. Sept 30th-Oct 5th, 2007. Poster session.
  • A Sensitive Subject: Happloinsufficiency of the V-ATPase. Northeast Regional Yeast Meeting (NERY), Roswell Park Comprehensive Cancer Center. October 13th, 2006. Poster session.
  • Genetic interactions among V-ATPase subunits. Northeast Regional Yeast Meeting (NERY), University of Rochester. October 5th, 2005. Poster session.

Awards/Honors

  • UB Biochemistry Dissertation Research Recognition Award, 2014
  • UB SMBS Dean's Award for Outstanding Dissertation Research, 2014
  • Central Society for Clinical Research (CSCR) Trainee Travel Award, 2012
  • Mark Diamond Research Grant, SUNY Buffalo GSA, 2012
  • PhRMA Foundation Pre-Doctoral Fellowship Award in Informatics, 2009-2011
  • Elizabeth Olmsted Ross Award for Outstanding Oral Presentation, SUNY Buffalo Biochemistry Dept., 2011
  • Syracuse University Scholar for Top 10 University graduate, 2006                                                
  • Phi Beta Kappa - National Honors Fraternity, 2006
  • David Orlin Award for Top Honors Thesis Overall, Syracuse University, 2006
  • Renee Crown Award for Top Honors Thesis in Natural Sciences, Syracuse University, 2006
  • Donald G. Lundgren Memorial Award for Outstanding Scholarship & Research, Syracuse University, 2006
  • Remembrance Scholarship, Syracuse University (2005)

Medical School Activities

  • MSTP Newsletter
  • Biochemistry Department Newsletter

Extracurricular Interests

Syracuse basketball, music, reading, exercise