Portrait of Mark Bucsek.

Mark J. Bucsek

Department

Immunology, Roswell Park Comprehensive Cancer Center

Thesis

Thesis title:  Understanding How Adrenergic Stress Signaling Impacts the Anti-Tumor Immune Response and the Efficacy of Checkpoint Inhibitors in Murine Tumor Models

Residency

Obstetrics-Gynecology, State University of New York Upstate Medical University

Undergraduate Education

Bioengineering, University of Pittsburgh

Thesis Advisor

Publications

  • Bucsek MJ, Qiao C, MacDonald C, Giridharan T, Evans L, Niedzwecki B, Liu H, Kokolus KM, Eng JW, Messmer MN, Attwood K, Abrams SI, Hylander BL, Repasky EA.  β-adrenergic signaling in mouse models housed at standard temperatures suppresses an effector phenotype in CD8+ T cells and undermines checkpoint inhibitor therapy.  Cancer Research.  2017.  (in revision)
  • Bucsek MJ, MacDonald C, Evans L, Qiao G, Niedzwecki B, Greenberg D, Hylander BL, Repasky EA.  β-adrenergic signaling drives tumor cell radioresistance and suppresses host immune responses in murine tumor models.  (In preparation)
  • Bucsek MJ, Giridharan T, MacDonald C, Repasky EA.  β-adrenergic receptor signaling influences immune cell behavior: implications for infection and autoimmunity.  International Journal of Hyperthermia.  2017.  Invited Review.  (In preparation)
  • Fumito Ito, Amy Ku, Mark J. Bucsek, Jason B. Muhitch, Trupti D. Vardam, Minhyung Kim, Daniel T. Fisher, Marta Camoriano, Thaer Khoury, Joseph J. Skitzki, Sandra O. Gollnick, and Sharon S. Evans. "Immune Adjuvant Activity of Pre-resectional Radiofrequency Ablation Protects against Local and Systemic Recurrence in an Aggressive Murine Colorectal Cancer Model."  PLoS One.  2015;10(11):e0143370.
  • Jason W.-L. Eng, Chelsea B. Reed, Kathleen M. Kokolus, Rosemarie Pitoniak, Adam Utley, Mark J. Bucsek, Wen Wee Ma, Elizabeth A. Repasky, and Bonnie L. Hylander.  Housing Temperature-Induced Stress Drives Therapeutic Resistance in Murine Models through β2-Adrenergic Receptor Activation.  Nature Communications, 2015. 6.
  • Joseph B. Vella, Seth. D. Thomson, Mark. J. Bucsek, Minjung Song, Johnny Huard.  Murine and Human Myogenic Cells Identified by Elevated Aldehyde Dehydrogenase Activity: Implications for Muscle Regeneration and Repair. Published, December 15, 2011. PLoS One.

Professional/Educational Meetings/Abstracts

  • Mark J. Bucsek.  “Thermoregulation and its Immuno-Oncology relevance in endogenous CD8+ T cell efficacy and immunotherapy. Invited Talk, 2nd Annual Immuno-Oncology Meeting of the Centro de Investigacion de Cancer en Sonora (CICS);  Ciudad Obregon, Sonora (Mexico).  2016.
  • Mark J. Bucsek, Guanxi Qiao, Cameron MacDonald, Lauren Evans, Bonnie L. Hylander, Elizabeth A. Repasky. “β-adrenergic signaling induced by cool housing temperatures mediates immune suppression and impairs the efficacy of anti-PD-1 checkpoint blockade immunotherapy in laboratory mice”.  Society for Immunotherapy of Cancer (SITC).  National Harbor, MD.  2016.
  • Mark J. Bucsek, Guanxi Qiao, Haichao Liu, Lauren Evans, Cameron MacDonald, Bonnie L. Hylander, Elizabeth A. Repasky. " The impact of β-adrenergic signaling on radioresistance and anti-tumor immunity in murine tumor models."  International Congress on Hyperthermic Oncology (ICHO).  New Orleans, LA.  2016.
  • Mark J. Bucsek, Guanxi Qiao, Haichao Liu, Lauren Evans, Cameron MacDonald, Bonnie L. Hylander, Elizabeth A. Repasky. "Adrenergic signaling mediates immune suppression induced by cool housing temperatures for laboratory mice."  American Association of Immunologists (AAI).  Seattle, WA.  2016.
  • Mark J. Bucsek, Guanxi Qiao, Haichao Liu, Lauren Evans, Cameron MacDonald, Bonnie L. Hylander, Elizabeth A. Repasky. "Adrenergic signaling mediates immune suppression induced by cool housing temperatures."  Tumor Immunology Department and Program Retreat.  Ellicottville, NY.  2016.
  • Guanxi Qiao, Mark J. Bucsek, Haichao Liu, Bonnie L. Hylander, Elizabeth A. Repasky. "Adrenergic signaling mediates immune suppression induced by cool housing temperatures."  Tumor Immunology Department and Program Retreat.  Ellicottville, NY.  2016.
  • Mark Bucsek, Guanxi Qiao, Haichao Liu, Lauren Evans, Cameron MacDonald, Timothy Winslow, Bonnie Hylander, Anurag Singh, Elizabeth Repasky.  "The impact of β-adrenergic signaling on radioresistance and anti-tumor immunity."  Society for Immunotherapy of Cancer (SITC).  National Harbor, MD.  2015.
  • Mark J. Bucsek, Timothy Winslow, Lauren Evans, Guanxi Qiao, Elizabeth A. Repasky.  β-adrenergic receptor signaling enhances tumor cell resistance to radiation in vitro.  60th Annual Meeting of the Radiation Research Society.  Weston, FL. 2015.
  • Mark J. Bucsek, Kathleen Kokolus, Jason Eng, Bonnie Hylander and Elizabeth Repasky.  Destressing Laboratory Mice Reveals New Relationships between Heat Production, Anti-tumor Immunity and β-Adrenergic Receptor Signaling.  32nd Annual Meeting of the Society for Thermal Medicine.  Orlando, FL. 2015.
  • Mark J. Bucsek, Kathleen Kokolus, Jason Eng, Bonnie Hylander and Elizabeth Repasky.  Destressing Laboratory Mice Reveals New Relationships between Heat Production, Anti-tumor Immunity and β-Adrenergic Receptor Signaling.  Mayville, NY.  2015.
  • Mark Bucsek, Fumito Ito, Jason Muhitch, Ashwin Ajith, Sharon Evans.  Radiofrequency ablation amplifies the efficacy of adoptive T cell transfer therapy in the generation of antitumor immunity.  31st Annual Meeting of the Society for Thermal Medicine.  Minneapolis, MN. 2014.
  • Mark Bucsek, Fumito Ito, Jason Muhitch, Ashwin Ajith, Sharon Evans.  Enhancing the antitumor effects of adoptive cell transfer immunotherapy using preconditioning thermal ablation.  Annual Meeting of the American Association of Immunologists.  Pittsburgh, PA. 2014.
  • Mark Bucsek, Fumito Ito, Jason Muhitch, Ashwin Ajith, Sharon Evans.  Overcoming hurdles to effective immunotherapy using radiofrequency ablation.  13th Annual Meeting of the Buffalo Conference on Immunology.  Mayville, NY. 2014.
  • Mark Bucsek, Fumito Ito, Jason Muhitch, Ashwin Ajith, Sharon Evans.  Overcoming hurdles to effective immunotherapy using radiofrequency ablation.  13th Annual Meeting of the Translational Research Cancer Center Consortium.  Seven Springs, PA. 2014.
  • Fumito Ito, Jason Muhitch, Mark Bucsek, Sharon Evans.  Synergistic Antitumor Activity of Thermal Ablation and Adoptive T Cell Transfer Cancer Immunotherapy. American College of Cryosurgery Annual Meeting. Key Largo, FL. 2014.
  • J.B. Vella, M. Song, M. J. Bucsek, J. Huard. Aldehyde Dehydrogenase: A Novel Marker for Muscle Derived Stem Cell Isolation. 57th Annual Meeting of the Orthopedic Research Society. Long Beach, CA. 2011.
  • M. J. Bucsek, J. B. Vella, B. Gharaibeh, J. Huard. Murine myoblasts with elevated aldehyde dehydrogenase levels have an improved oxidative and imflammatory stress resistance leading to superior proliferation and differentiation under stress. Pittsburgh Tissue Engineering Initiative Summer Conference. Pittsburgh, PA. 2010.
  • M. J. Bucsek, J. B. Vella, B. Charaibeh, J. Huard. Muscle derived cells with high levels of aldehyde dehydrogenase are endowed with an improved oxidative and inflammatory stress resistance. Pittsburgh Tissue Engineering Initiative Summer Conference. Pittsburgh, PA. 2010.
  • M. J. Bucsek, J. B. Vella, B. Gharaibeh, J. Huard. Murine myoblasts with elevated aldehyde dehydrogenase levels have an increased oxidative and inflammatory stress resistance. Pittsburgh Tissue Engineering Initiative Summer Conference. Pittsburgh, PA. 2010
  • J.B. Vella, M. Song, M. J. Bucsek, J. Huard. Enhanced stress resistance and  muscle regeneration capacity of human and murine muscle derived cells with elevated aldehyde dehydrogenase expression. FASEB Skeletal Muscle, Satellite and Stem Cells Conference. Carefree, Arizona. 2010
  • J. B. Vella, M. J. Bucsek, J. Huard. The use of aldehyde dehydrogenase (ALDH) expression as a marker for muscle derived cells endowed with high survival and myogenic potential. 56th Annual Meeting of the Orthopedic Research Society.  New Orleans, LA. 2010.
  • J. B. Vella, M. J. Bucsek, J. Huard. Murine muscle derived cells expressing high aldehyde dehydrogenase (ALDH) display increased cell survival and myogenic differentiation potential. 56th Annual Meeting of the Orthopedic Research Society.  New Orleans, LA. 2010.
  • M. J. Bucsek, R. Zhao, M. Kameneva. Microrehological characterization of red blood cells grown ex vivo. Undergraduate Intramural Internship Fall Conference. Pittsburgh, PA. 2009.

Awards/Honors

  • Invited Speaker - 2nd Annual Immuno-Oncology Meeting of the Centro de Investigacion de Cancer en Sonora (CICS);  Ciudad Obregon, Sonora (Mexico).  (2016)
  • Recipient of NIH Young Investigator Award - ICHO (2016)
  • Recipient of NIH Young Investigator Award - TRC3 (2016)
  • Roswell Park Department of Immunology T-32 Training Grant (2015 - present)
  • Invited Reviewer - International Journal of Hyperthermia (2015 - present)
  • Recipient of NIH Young Invest Award - STM (2015)
  • Runner-up - Best Graduate Student Presentation - BIC (2015)
  • Recipient of the Outstanding Student of Bioengineering of the Cellular and Medical Products Award for the Pitt Bioengineering class of 2011
  • Graduated from the University of Pittsburgh Summa Cum Laude

Medical School Activities

  • UB HEALS (2017 - present)
  • Graduate Student Association - Department of Immunology (Senator, 2013 - 2014)
  • MD-PhD Polity Representative (2013 - 2014)
  • Oncology Interest Group (Co-President, 2012 - 2014)
  • Surgery Interest Group (2011 - Present)

Extracurricular Interests

  • Erie County Opiate Epidemic Task Force, active member
  • Playing and writing Music
  • Spending time with family and friends
  • Pittsburgh Steelers Football
  • Traveling
  • Skiing
  • Playing sports