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Christopher                    Cohan

Christopher S. Cohan PhD

Department of Pathology and Anatomical Sciences

Professor

 
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Professional Summary:

Experiments focus on the dynamics of microtubule assembly in spindles during the process of meiosis using live imaging of labeled microtubules. We utilize cranefly spermatocytes isolated into acute cultures containing cells at various stages of cell division that can be studied for several hours. Microtubule dynamics are studied by the technique of fluorescent speckle imaging whereby spermatocytes are injected with low concentrations of fluorescent-labeled tubulin that incorporates into microtubules at low density. This creates a patterned, discontinuous labeling of microtubules that enables quantification of the rates and sites of assembly. We currently are testing the role of tension as a modulator of microtubule polymerization during anaphase. Chromosome kinetochores exert dragging forces on attached microtubule ends favoring microtubule assembly as chromosomes move from spindle equator to spindle poles. However, microtubule disassembly is induced when dragging forces are eliminated by laser ablation of attached chromosomes. These observations may reveal some of the self-organizing mechanisms that control the orderly separation of chromosomes during cell division.

Education and Training:
  • Dipl, Educational Informatics, University at Buffalo (2008)
  • Ph.D., Anatomy, Case Western Reserve University (1980)
  • BS, Biology/Mathematics, University at Albany, SUNY (1974)
Employment:
  • Professor, Pathology and Anatomical Sciences, University at Buffalo (2005-present)
  • Associate Professor, Anatomy and Cell Biology, University at Buffalo (1992–2005)
  • Assistant Professor, Anatomy and Cell Biology, University at Buffalo (1986–1992)
  • Post Doctoral Fellow, Biological Sciences, University of Iowa (1980–1986)
Awards and Honors:
  • Siegel Teaching Award (2012)
  • SUNY Chancellor's Award for Excellence in Teaching (2005)
  • Induction into Alpha Omega Alpha (AOA) (2005)

Research Expertise:
  • Cytoskeletal Dynamics

Journal Articles:
  • LaFountain JR, Cohan CS, Oldenbourg R. Pac-man motility of kinetochores unleashed by laser microsurgery. Mol Biol Cell. 2012; 23(16).
  • LaFountain JR, Cohan CS, Oldenbourg R. Functional states of kinetochores revealed by laser microsurgery and fluorescent speckle microscopy. Mol Biol Cell. 2011; 22(24).
  • Torreano PJ, Waterman-Storer CM, Cohan CS. The effects of collapsing factors on F-actin content and microtubule distribution of Helisoma growth cones. Cell Motil Cytoskeleton. 2005; 60(3).
  • LaFountain JR, Cohan CS, Siegel AJ, LaFountain DJ. Direct visualization of microtubule flux during metaphase and anaphase in crane-fly spermatocytes. Mol Biol Cell. 2004; 15(12).
  • Zhou FQ, Cohan CS. How actin filaments and microtubules steer growth cones to their targets.. J Neurobiol. 2004; 58(1).
  • Torreano PJ, Cohan CS. Calcium and voltage dependent inactivation of sodium and calcium currents limits calcium influx in Helisoma neurons.. J Neurobiol. 2003; 54(3).
  • Adams MC, Salmon WC, Gupton SL, Cohan CS, Wittmann T, Prigozhina N, Waterman-Storer CM. A high-speed multispectral spinning-disk confocal microscope system for fluorescent speckle microscopy of living cells.. Methods. 2003; 29(1).
  • Zhou FQ, Waterman-Storer CM, Cohan CS. Focal loss of actin bundles causes microtubule redistribution and growth cone turning.. J Cell Biol. 2002; 157(5).
  • Zhou FQ, Cohan CS. Growth cone collapse through coincident loss of actin bundles and leading edge actin without actin depolymerization.. J Cell Biol. 2001; 153(5).
  • Cohan CS, Welnhofer EA, Zhao L, Matsumura F, Yamashiro S. Role of the actin bundling protein fascin in growth cone morphogenesis: localization in filopodia and lamellipodia.. Cell Motil Cytoskeleton. 2001; 48(2).
  • Hashino E, Johnson EM, Milbrandt J, Shero M, Salvi RJ, Cohan CS. Multiple actions of neurturin correlate with spatiotemporal patterns of Ret expression in developing chick cranial ganglion neurons.. J Neurosci. 1999; 19(19).
  • Welnhofer EA, Zhao L, Cohan CS. Calcium influx alters actin bundle dynamics and retrograde flow in Helisoma growth cones.. J Neurosci. 1999; 19(18).
  • Herring TL, Cohan CS, Welnhofer EA, Mills LR, Morris CE. F-actin at newly invaginated membrane in neurons: implications for surface area regulation.. J Membr Biol. 1999; 171(2).
  • Torreano PJ, Cohan CS. Electrically induced changes in Ca2+ in Helisoma neurons: regional and neuron-specific differences and implications for neurite outgrowth.. J Neurobiol. 1997; 32(2).
  • Zou JY, Cohan C, Rabin RA, Pentney RJ. Continuous exposure of cultured rat cerebellar macroneurons to ethanol-depressed NMDA and KCl-stimulated elevations of intracellular calcium.. Alcohol Clin Exp Res. 1995; 19(4).
  • Williams DK, Cohan CS. Calcium transients in growth cones and axons of cultured Helisoma neurons in response to conditioning factors.. J Neurobiol. 1995; 27(1).
  • Williams DK, Cohan CS. The role of conditioning factors in the formation of growth cones and neurites from the axon stump after axotomy.. Brain Res Dev Brain Res. 1994; 81(1).
  • Bianchi LM, Cohan CS. Developmental regulation of a neurite-promoting factor influencing statoacoustic neurons.. Brain Res Dev Brain Res. 1991; 64(1-2).
  • Polak KA, Edelman AM, Wasley JW, Cohan CS. A novel calmodulin antagonist, CGS 9343B, modulates calcium-dependent changes in neurite outgrowth and growth cone movements.. J Neurosci. 1991; 11(2).
  • Cohan CS, Errick JE, Xia MH. Formation of electrical connections between cultured identified neurons and muscle fibers of the snail Helisoma.. Brain Res Dev Brain Res. 1990; 56(1).
  • Cohan CS, Connor JA, Kater SB. Electrically and chemically mediated increases in intracellular calcium in neuronal growth cones.. J Neurosci. 1987; 7(11).
See All (21 Total) >

Service Activities:
  • Curator, Museum of Neuroanatomy; Director (2008)

Clinical Specialties:
Clinical Offices:
Insurance Accepted:

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Contact Information

323 Sherman Hall
Buffalo, NY 14214
Phone: (716) 829-3081
Email: ccohan@buffalo.edu


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