Lee Ann Garrett-Sinha PhD
Lee Ann Garrett-Sinha
PhD
Professor
Jacobs School of Medicine & Biomedical Sciences
Specialty/Research Focus
Autoimmunity; Cell growth, differentiation and development; Gene Expression; Immunology; Molecular and Cellular Biology; Molecular genetics; Signal Transduction; Transcription and Translation; Transgenic organisms
Education and Training:
- Postdoctoral Fellow, Immunology, University of Chicago (2001)
- PhD, Molecular Biology, University of Texas (1996)
- BA, Biology/Biochemistry, Rice University (1989)
Employment:
- Professor, Biochemistry, State University of New York at Buffalo (2017-present)
- Associate Professor, Biochemistry, SUNY at Buffalo (2011-present)
- Assistant Professor, Biochemistry, SUNY at Buffalo (2001–2011)
- Postdoctoral Research Associate, Department of Medicine, University of Chicago (1999–2001)
- Post Doctoral Fellow, Department of Medicine, Howard Hughes Medical Institute at the University of Chicago (1996–1999)
- Graduate Student, Molecular Genetics, University of Texas, Health Sciences Center at Houston (1990–1996)
- Research Technician, M.D. Anderson Cancer Center (1988–1990)
Awards and Honors:
- Biosymposia Travel Award (2008)
- NIH Travel Award (2007)
- Young Investigator Travel Award (2006)
- Brian D. Novis Research Grant Recipient 2003 (2003)
Research Expertise:
- Autoimmune Disease: Analysis of Autoantibody Levels Analysis of B and T cell Activation Analysis of B cell tolerance to self-antigen
- B cell receptor signaling: Western blotting Immunoprecipitation Ca2+ mobilization
- Squamous Cell Carcinoma: Mouse models of Squamous cell cancer Analysis of Skin phenotypes
- Transgenic/Knockout Mice: Generation, breeding and analysis of transgenic and knockout mouse strains
Research Centers:
- Witebsky Center for Microbial Pathogenesis and Immunology
- Center of Excellence in Bioinformatics and Life Sciences
UB 2020 Strategic Strengths:
- Molecular Recognition in Biological Systems and Bioinformatics
Grants and Sponsored Research:
- April 2016–March 2021
Regulation and Consequences of Ets1 Downregulation in B Cells
NIH
Role: Co-Investigator - January 2016–December 2018
Understanding the Pathway Regulated by the Lupus Susceptibility Gene Ets1
Lupus Research Alliance
Role: Co-Investigator - May 2010–April 2015
Identification of Specific Roles for Ets-1 in B Cell Tolerance
NIAID, NIH
Role: Principal Investigator - April 2012–March 2014
Functional Relationships Between the Lupus Susceptibility Loci Lyn and Ets1
National Institutes of Health
Role: Co-Investigator - August 2011–July 2013
Functional Relationships Between the Lupus Susceptibility Loci Lyn and Ets1
Alliance for Lupus Research
Role: Co-Investigator
Journal Articles:
- Noviski M, Mueller JL, Satterthwaite A, Garrett-Sinha LA, Brombacher F, Zikherman J, Garrett-Sinha L. IgM and IgD B cell receptors differentially respond to endogenous antigens and control B cell fate. Elife. 2018; 7.
- Willis SN, Tellier J, Liao Y, Trezise S, Light A, O'Donnell K, Garrett-Sinha LA, Shi W, Tarlinton DM, Nutt SL, Garrett-Sinha L. Environmental sensing by mature B cells is controlled by the transcription factors PU.1 and SpiB. Nat Commun. 2017; 8(1).
- Saelee P, Kearly A, Nutt SL, Garrett-Sinha LA, Garrett-Sinha L. Genome-Wide Identification of Target Genes for the Key B Cell Transcription Factor Ets1. Front Immunol. 2017; 8.
- Garrett-Sinha LA, Kearly A, Satterthwaite AB, Garrett-Sinha L. The Role of the Transcription Factor Ets1 in Lupus and Other Autoimmune Diseases. Crit Rev Immunol. 2016; 36(6).
- Russell L, John S, Cullen J, Luo W, Shlomchik MJ, Garrett-Sinha LA, Garrett-Sinha L. Requirement for Transcription Factor Ets1 in B Cell Tolerance to Self-Antigens. J Immunol. 2015; 195(8).
- Mayeux J, Skaug B, Luo W, Russell LM, John S, Saelee P, Abbasi H, Li QZ, Garrett-Sinha LA, Satterthwaite AB, Garrett-Sinha L. Genetic Interaction between Lyn, Ets1, and Btk in the Control of Antibody Levels. J Immunol. 2015; 195(5).
- Rozanski CH, Utley A, Carlson LM, Farren MR, Murray M, Russell LM, Nair JR, Yang Z, Brady W, Garrett-Sinha LA, Schoenberger SP, Green JM, Boise LH, Lee KP, Garrett-Sinha L. CD28 Promotes Plasma Cell Survival, Sustained Antibody Responses, and BLIMP-1 Upregulation through Its Distal PYAP Proline Motif. J Immunol. 2015; 194(10).
- Yuan X, Garrett-Sinha LA, Sarkar D, Yang S, Garrett-Sinha L. Deletion of IFT20 in early stage T lymphocyte differentiation inhibits the development of collagen-induced arthritis. Bone Res. 2014; 2.
- Luo W, Mayeux J, Gutierrez T, Russell L, Getahun A, Müller J, Tedder T, Parnes J, Rickert R, Nitschke L, Cambier J, Satterthwaite AB, Garrett-Sinha LA, Garrett-Sinha L. A balance between B cell receptor and inhibitory receptor signaling controls plasma cell differentiation by maintaining optimal Ets1 levels. J Immunol. 2014; 193(2).
- John S, Russell L, Chin SS, Luo W, Oshima R, Garrett-Sinha LA, Garrett-Sinha L. Transcription factor Ets1, but not the closely related factor Ets2, inhibits antibody-secreting cell differentiation. Mol Cell Biol. 2014; 34(3).
- Agudo J, Ruzo A, Tung N, Salmon H, Leboeuf M, Hashimoto D, Becker C, Garrett-Sinha LA, Baccarini A, Merad M, Brown BD, Garrett-Sinha L. The miR-126-VEGFR2 axis controls the innate response to pathogen-associated nucleic acids. Nat Immunol. 2014; 15(1).
- Chin SS, Romano RA, Nagarajan P, Sinha S, Garrett-Sinha LA, Garrett-Sinha L. Aberrant epidermal differentiation and disrupted DeltaNp63/Notch regulatory axis in Ets1 transgenic mice. Biol Open. 2013; 2(12).
- Huntoon KM, Russell L, Tracy E, Barbour KW, Li Q, Shrikant PA, Berger FG, Garrett-Sinha LA, Baumann H, Garrett-Sinha L. A unique form of haptoglobin produced by murine hematopoietic cells supports B-cell survival, differentiation and immune response. Mol Immunol. 2013; 55(3-4).
- Garrett-Sinha LA, Garrett-Sinha L. Review of Ets1 structure, function, and roles in immunity. Cell Mol Life Sci. 2013; 70(18).
- DeKoter RP, Geadah M, Khoosal S, Xu LS, Thillainadesan G, Torchia J, Chin SS, Garrett-Sinha LA, Garrett-Sinha L. Regulation of follicular B cell differentiation by the related E26 transformation-specific transcription factors PU.1, Spi-B, and Spi-C. J Immunol. 2010; 185(12).
- Nagarajan P, Chin SS, Wang D, Liu S, Sinha S, Garrett-Sinha LA, Garrett-Sinha L. Ets1 blocks terminal differentiation of keratinocytes and induces expression of matrix metalloproteases and innate immune mediators. J Cell Sci. 2010; 123(Pt 20).
- Russell L, Garrett-Sinha LA, Garrett-Sinha L. Transcription factor Ets-1 in cytokine and chemokine gene regulation. Cytokine. 2010; 51(3).
- Ye M, Coldren C, Liang X, Mattina T, Goldmuntz E, Benson DW, Ivy D, Perryman MB, Garrett-Sinha LA, Grossfeld P,. Deletion of ETS-1, a gene in the Jacobsen syndrome critical region, causes ventricular septal defects and abnormal ventricular morphology in mice. Hum Mol Genet. 2010; 19(4).
- John SA, Garrett-Sinha LA, Garrett-Sinha L. Blimp1: a conserved transcriptional repressor critical for differentiation of many tissues. Exp Cell Res. 2009; 315(7).
- Nagarajan, P., Parikh, N., Garrett-Sinha, L.A., Sinha, S.. Ets1 induces dysplastic changes when expressed in terminally-differentiating squamous epithelial cells PLoS One. 2009; 4(1).
- Nagaleekar VK, Diehl SA, Juncadella I, Charland C, Muthusamy N, Eaton S, Haynes L, Garrett-Sinha LA, Anguita J, Rincón M, Garrett-Sinha L. IP3 receptor-mediated Ca2+ release in naive CD4 T cells dictates their cytokine program. J Immunol. 2008; 181(12).
- Parikh, N., Nagarajan, P., Sei-Ichi, M., Sinha, S., Garrett-Sinha, L.A.. Isolation and characterization of an immortalized oral keratinocyte cell line of mouse origin Arch Oral Biol. 2008; 53(11).
- Landesberg R, Cozin M, Cremers S, Woo V, Kousteni S, Sinha S, Garrett-Sinha L, Raghavan S. Inhibition of oral mucosal cell wound healing by bisphosphonates. J Oral Maxillofac Surg. 2008; 66(5).
- John, S.A., Clements, J.L., Russell, L.M., Garrett-Sinha, L.A.. Ets-1 regulates plasmacytic differentiation by interfering with the activity of the transcription factor Blimp-1 J Biol Chem. 2008; 283(2).
- Clements, J.L., John, S.A., Garrett-Sinha, L.A.. Impaired generation of CD8 thymocytes in Ets-1-deficient mice J Immunol. 2006; 177(2).
- Wang, D., John, S.A., Clements, J.L., Percy, D.H., Barton, K.P., Garrett-Sinha, L.A.. Ets-1 deficiency leads to altered B cell differentiation, hyper-responsiveness to TLR9 and autoimmune disease Int Immunol. 2005; 17(9).
- Garrett-Sinha LA, Hou, P., Wang, D., Grabiner, B., Araujo, E., Rao, S., Yun, T.J., Clark, E.A., Simon, M.C., Clark, M.R.. Spi-1 and Spi-B control the expression of the Grap2 gene in B cells Gene. 2005; 353(1).
- Lefebvre, J.M., Haks, M.C., Carleton, M.O., Rhodes, M., Simon, M.C., Eisenlohr, L.C., Garrett-Sinha, L.A., Wiest, D.L.. Enforced expression of Spi-B reverses T lineage commitment and blocks beta-selection J Immunol. 2005; 174(10).
- Hu CJ, Rao S, Ramirez-Bergeron DL, Garrett-Sinha LA, Gerondakis S, Clark MR, Simon MC. PU.1/Spi-B regulation of c-rel is essential for mature B cell survival. Immunity. 2001; 15(4).
- Garrett-Sinha LA, Dahl R, Rao S, Barton KP, Simon MC. PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1. Blood. 2001; 97(9).
- Fischer EA, Verpont MC, Garrett-Sinha LA, Ronco PM, Rossert JA. Klf6 is a zinc finger protein expressed in a cell-specific manner during kidney development. J Am Soc Nephrol. 2001; 12(4).
- Rao S, Garrett-Sinha LA, Yoon J, Simon MC, Sinha LA. The Ets factors PU.1 and Spi-B regulate the transcription in vivo of P2Y10, a lymphoid restricted heptahelical receptor.. J Biol Chem. 1999; 274(48).
- Garrett-Sinha LA, Su GH, Rao S, Kabak S, Hao Z, Clark MR, Simon MC, Sinha LA. PU.1 and Spi-B are required for normal B cell receptor-mediated signal transduction.. Immunity. 1999; 10(4).
- Inagaki Y, Truter S, Bou-Gharios G, Garrett LA, de Crombrugghe B, Nemoto T, Greenwel P, Sinha LA. Activation of Proalpha2(I) collagen promoter during hepatic fibrogenesis in transgenic mice.. Biochem Biophys Res Commun. 1998; 250(3).
- Su GH, Chen HM, Muthusamy N, Garrett-Sinha LA, Baunoch D, Tenen DG, Simon MC, Sinha LA. Defective B cell receptor-mediated responses in mice lacking the Ets protein, Spi-B.. EMBO J. 1997; 16(23).
- Garrett-Sinha LA, Eberspaecher H, Seldin MF, de Crombrugghe B, Sinha LA. A gene for a novel zinc-finger protein expressed in differentiated epithelial cells and transiently in certain mesenchymal cells.. J Biol Chem. 1996; 271(49).
- Bou-Gharios G, Garrett LA, Rossert J, Niederreither K, Eberspaecher H, Smith C, Black C, Crombrugghe B, Sinha LA. A potent far-upstream enhancer in the mouse pro alpha 2(I) collagen gene regulates expression of reporter genes in transgenic mice.. J Cell Biol. 1996; 134(5).
- Hasegawa T, Zhou X, Garrett LA, Ruteshouser EC, Maity SN, de Crombrugghe B, Sinha LA. Evidence for three major transcription activation elements in the proximal mouse proalpha2(I) collagen promoter.. Nucleic Acids Res. 1996; 24(16).
- Rossert JA, Garrett LA, Sinha LA. Regulation of type I collagen synthesis.. Kidney Int Suppl. 1995; 49.
- Goldberg H, Helaakoski T, Garrett LA, Karsenty G, Pellegrino A, Lozano G, Maity S, de Crombrugghe B, Sinha LA. Tissue-specific expression of the mouse alpha 2(I) collagen promoter. Studies in transgenic mice and in tissue culture cells.. J Biol Chem. 1992; 267(27).
Professional Memberships:
- American Society for Microbiology (2013–2014)
- New York Academy of Sciences (2009–2010)
- Developmental Genomics Research Focus Group (2009–2014)
- Buffalo Collegium of Immunologists (2006–2014)
- American Association of Immunologists; Major American Immunology Society (2002–2014)
Service Activities:
- Reviews Editor, Frontiers in Immunology; Reviewer (2017–2018)
- Associate Editor, Journal of Immunology; Editor (2014)
- IACUC Committee; For Roswell Park IACUC; Member (2008–2011)
- Careers Roundtable Discussion Leader; AAI Annual meeting in 2007; Contributor (2007)
- Organizing Committee Buffalo Immunology Conference; Chair (2005–2014)
- Faculty Council Alternate; Alternate to faculty Council; Committee Member (2004–2006)
- Member Search Committee for Department of Biology Faculty Search; To select and interview candidates for faculty search in the Biology Department; Member (2003–2004)