University at Buffalo (UB)
Andrew M. Gulick PhD
Andrew M. Gulick
PhD
Professor
Department of Structural Biology
Jacobs School of Medicine & Biomedical Sciences
Specialty/Research Focus
Bacterial Pathogenesis; Infectious Disease; Metalloenzymes; Microbial Pathogenesis; Microbiology; Protein Function and Structure; Structural Biology
Professional Summary:
My research program aims to understand how bacteria produce natural products, small molecules that are secreted from the cell to adapt to diverse environments. These molecules allow the bacteria to compete with other microbes or, in the host-pathogen setting, to establish or exacerbate an infection. Natural product biosynthesis may therefore serve as a target for antimicrobial development.
My lab uses a variety of techniques to examine these pathways. A core approach is to use X-ray crystallography to determine the molecular structure of proteins that catalyze important steps in natural product biosynthesis. Structural observations are tested and validated using biochemical techniques to examine the catalytic reactions. Finally, molecular and cellular techniques are used to examine biosynthetic gene cluster activity in the cell. These studies will inform efforts to engineer enzymes to produce novel natural product and identify new products of previously uncharacterized pathways.
I have a long-standing interest in the Nonribosomal Peptide Synthetases (NRPSs), a family of large, multidomain enzymes that produce important peptide natural products like the antibiotic vancomycin or the anticancer agent bleomycin. NRPSs operate like an assembly line in which the nascent peptide is attached to a carrier domain that shuttles the synthetic intermediates to neighboring catalytic domains. The carrier and catalytic domains are often joined in a single polypeptide that is thousands of residues in length. By examine the crystal structures of large NRPS proteins, we have determined some of the features that enable this fascinating biosynthetic mechanism.
Many NRPS products are siderophores, small molecules that bind iron and are required for growth in the pathogenic environment. My lab also studies aerobactin, an NRPS-independent siderophore pathway that is a virulence factor for hypervirulent Klebsiella pneumoniae. We have biochemically and structurally characterized the aerobactin biosynthetic pathway and have developed an approach to find inhibitors of aerobactin biosynthesis that may be tools to probe the pathway chemically to inhibit growth of this human pathogen.
Education and Training:
- PhD, Experimental Oncology and Biochemistry, University of Wisconsin (1995)
- BS, Biochemistry, Brown University (1989)
- Postdoctoral Fellow, Biochemistry, University of Wisconsin
Employment:
- Director, PhD Program in Biomedical Sciences, Jacobs School of Medicine and Biomedical Sciences (2020-present)
- Professor, Structural Biology, University at Buffalo Jacobs School of Medicine & Biomedical Sciences (2021-present)
- Director, Natural Sciences Interdisciplinary Master, Jacobs School of Medicine and Biomedical Sciences (2020–2022)
- Associate Professor, Structural Biology, University at Buffalo Jacobs School of Medicine & Biomedical Sciences (2018–2021)
- VP for Research, Hauptman-Woodward Institute (2015–2017)
- Principal Research Scientist, Hauptman-Woodward Institute (2012–2017)
- Research Assistant Professor, Structural Biology, University at Buffalo Jacobs School of Medicine & Biomedical Sciences (2001–2017)
- Senior Research Scientist, Hauptman-Woodward Institute (2007–2012)
- Research Scientist, Hauptman-Woodward Institute (2001–2007)
- Assistant Scientist, Biochemistry, University of Wisconsin (1998–2001)
Research Expertise:
- Assay Development: We develop approaches to identify chemical probes that block enzyme reactions that can be used to improve our understanding of the role that proteins play in virulence.
- Enzymology: We use biochemical and structural techniques to identify the mechanisms that proteins use to catalyze critical reactions.
- Natural Product Biosynthesis: Many microbes use novel enzymes to produce small chemicals that are secreted into the environment and help the producing organism adapt to diverse environments. We use structural, chemical, and biological tools to identify natural products and the pathways for their biosynthesis.
- Structural Biology: We use x-ray crystallography to determine the molecular structure of important macromolecules that carry out critical biological processes.
Grants and Sponsored Research:
- April 2020–March 2025
Structural Studies of Nonribosomal Peptide Synthesis (R35GM136235)
NIH/NIGMS
Role: Principal Investigator - January 2016–December 2019
The structural basis for modular nonribosomal peptide synthesis(R01GM116957)
NIH/NIGMS
Role: Principal Investigator - February 2016–January 2019
Development of HTP Assay for Inhibitors of Aerobactin Production(R01AI116998)
National Institutes of Health
Role: Principal Investigator - June 2012–May 2016
Enzymes involved in production of bacterial isonitrile-containing natural products [1158169].
National Science Foundation
Role: Principal Investigator - September 2011–October 2014
Identification of New Drug Targets in Multi-Drug Resistant Bacterial Infections
Department of Defense (DOD)
Role: Principal Investigator
$1,744,854 - July 2010–June 2014
Structures of Peptide Synthetases and Related Proteins [NIH(GM069440-06)]
National Institutes of Health
Role: Principal Investigator - July 2009–June 2010
Structures of Peptide Synthetases and Related Proteins [NIH(GM069440-S2)]
National Institutes of Health
Role: Principal Investigator - July 2004–June 2010
Structures of Peptide Synthetases and Related Proteins [NIH(GM069440)]
National Institutes of Health
Role: Principal Investigator - November 2008–November 2009
Development of a small molecule screening capability for structure-based drug design at the Hauptman-Woodward Institute
Max and Victoria Dreyfus Foundation
Role: Principal Investigator - July 2007–June 2009
Structure and function studies of Pseudomonas pyoverdine synthesis enzymes [CFF Gulick07I0]
Cystic Fibrosis Foundation
Role: Principal Investigator
Patents:
- Novel Glutathione S-transferase isoforms Mutant forms of mammalian glutathione s-transferase engineered to exhibit higher catalytic efficiency with a anti-cancer agent (2000)
Journal Articles:
- Drake EJ, Miller BR, Shi C, Tarrasch JT, Sundlov JA, Allen CL, Skiniotis G, Aldrich CC, Gulick AM. (2016) Structures of two distinct conformations of holo-non-ribosomal peptide synthetases. Nature (Jan), 529(7585): 235-238.
- Patel KD, Gulick AM. (2023) Structural and functional insights into d-poly-L-ornithine polymer biosynthesis from Acinetobacter baumannii. Communications Biology (Sep), 6(1): 982. doi:10.1038/s42003-023-05362-4
- Patel KD, MacDonald MR, Ahmed SF, Singh J, Gulick AM. (2023) Structural advances toward understanding the catalytic activity and conformational dynamics of modular nonribosomal peptide synthetases. Natural Product Reports (Apr)(40): 1550-1582. doi:10.1039/d3np00003f
- Patel KD, Ahmed SF, MacDonald MR, Gulick AM. (2023) Structural Studies of Modular Nonribosomal Peptide Synthetases. Methods in Molecular Biology (Clifton, N.J.) (Jan), 2670: 17-46.
- Yang J, Banas VS, Patel KD, Rivera GSM, Mydy LS, Gulick AM, Wencewicz TA. (2022) An acyl-adenylate mimic reveals the structural basis for substrate recognition by the iterative siderophore synthetase DesD. The Journal of Biological Chemistry (Jun)102166.
- Koeller CM, Smith TK, Gulick AM, Bangs JD. (2021) p67: a cryptic lysosomal hydrolase in Trypanosoma brucei?. Parasitology (Sep), 148(10): 1271-1276.
- Patel KD, Gulick AM. (2021) Anti-Zika candidates from a marine fungus with a remarkable biosynthetic repertoire. The Journal of Biological Chemistry (Aug), 297(3): 101047.
- Swayambhu G, Bruno M, Gulick AM, Pfeifer BA. (2021) Siderophore natural products as pharmaceutical agents. Curr Opin Biotechnol (Feb), 69: 242-251.
- Mhashal AR, Romero-Rivera A, Mydy LS, Cristobal JR, Gulick AM, Richard JP, Kamerlin SCL. (2020) Modeling the Role of a Flexible Loop and Active Site Side Chains in Hydride Transfer Catalyzed by Glycerol-3-phosphate Dehydrogenase. ACS Catal (Oct), 10(19): 11253-11267.
- Alexander EM, Kreitler DF, Guidolin V, Hurben AK, Drake EJ, Villalta PW, Balbo S, Gulick AM, Aldrich CC. (2020) Biosynthesis, Mechanism of Action, and Inhibition of the Enterotoxin Tilimycin Produced by the Opportunistic Pathogen Klebsiella oxytoca. ACS Infect Dis (Jun).
- Mydy LS, Bailey DC, Patel KD, Rice MR, Gulick AM. (2020) The Siderophore Synthetase IucA of the Aerobactin Biosynthetic Pathway Uses an Ordered Mechanism. Biochemistry (May), 59.
- Shi C, Miller BR, Alexander EM, Gulick AM, Aldrich CC. (2020) Design, Synthesis, and Biophysical Evaluation of Mechanism-Based Probes for Condensation Domains of Nonribosomal Peptide Synthetases. ACS Chem Biol (Jan), 15(7): 1813-1819.
- Branchini BR, Fontaine DM, Southworth TL, Huta BP, Racela A, Patel KD, Gulick AM. (2019) Mutagenesis and Structural Studies Reveal the Basis for the Activity and Stability Properties that Distinguish the Photinus Luciferases scintillans and pyralis. Biochemistry (Sep), 40.
- Patel KD, d'Andrea FB, Gaudelli NM, Buller AR, Townsend CA, Gulick AM. (2019) Structure of a bound peptide phosphonate reveals the mechanism of nocardicin bifunctional thioesterase epimerase-hydrolase half-reactions. Nat Commun (Aug), 10(1): 3868-3868.
- Kreitler DF, Gemmell EM, Schaffer JE, Wencewicz TA, Gulick AM. (2019) The structural basis of N-acyl-a-amino-ß-lactone formation catalyzed by a nonribosomal peptide synthetase. Nat Commun (Jul), 10(1): 3432-3432.
- Russo TA, Gulick AM. (2019) Aerobactin Synthesis Proteins as Antivirulence Targets in Hypervirulent Klebsiella pneumoniae. ACS Infect Dis (Apr).
- Gulick AM. (2019) Enzymes engineered to trap reaction intermediates. Nature (Jan), 565(7737): 28-29.
- Mydy LS, Cristobal JR, Katigbak RD, Bauer P, Reyes AC, Kamerlin SCL, Richard JP, Gulick AM. (2019) Human Glycerol 3-Phosphate Dehydrogenase: X-ray Crystal Structures That Guide the Interpretation of Mutagenesis Studies. Biochemistry (Jan).
- Bailey DC, Buckley BP, Chernov MV, Gulick AM. (2018) Development of a High-Throughput Biochemical Assay to Screen for Inhibitors of Aerobactin Synthetase IucA. SLAS Discov (Dec), 23(10): 1070-1082.
- Bailey DC, Bohac TJ, Shapiro JA, Giblin DE, Wencewicz TA, Gulick AM. (2018) Crystal Structure of the Siderophore Binding Protein BauB Bound to an Unusual 2:1 Complex Between Acinetobactin and Ferric Iron. Biochemistry (Nov), 57: 6653-6661.
- Gulick AM, Aldrich CC. (2018) Trapping interactions between catalytic domains and carrier proteins of modular biosynthetic enzymes with chemical probes. Nat Prod Rep (Nov), 35(11): 1156-1184.
- Ray S, Kreitler DF, Gulick AM, Murkin AS. (2018) The Nitro Group as a Masked Electrophile in Covalent Enzyme Inhibition. ACS Chem Biol (Jun), 13(6): 1470-1473.
- Bailey DC, Alexander E, Rice MR, Drake EJ, Mydy LS, Aldrich CC, Gulick AM. (2018) Structural and functional delineation of aerobactin biosynthesis in hypervirulent Klebsiella pneumoniae. J Biol Chem (May), 293(20): 7841-7852.
- Gulick AM. (2017) Nonribosomal peptide synthetase biosynthetic clusters of ESKAPE pathogens. Nat Prod Rep (Aug), 34(8): 981-1009.
- Branchini BR, Southworth TL, Fontaine DM, Murtiashaw MH, McGurk A, Talukder MH, Qureshi R, Yetil D, Sundlov JA, Gulick AM. (2017) Cloning of the Orange Light-Producing Luciferase from Photinus scintillans-A New Proposal on how Bioluminescence Color is Determined. Photochem Photobiol (Mar), 93(2): 479-485.
- Clevenger KD, Mascarenhas R, Catlin D, Wu R, Kelleher NL, Drake EJ, Gulick AM, Liu D, Fast W. (2017) Substrate Trapping in the Siderophore Tailoring Enzyme PvdQ. ACS Chem Biol (Mar), 12(3): 643-647.
- Miller BR, Drake EJ, Shi C, Aldrich CC, Gulick AM. (2016) Structures of a Nonribosomal Peptide Synthetase Module Bound to MbtH-like Proteins Support a Highly Dynamic Domain Architecture. J Biol Chem (Oct), 291(43): 22559-22571.
- Gulick AM. (2016) Structural insight into the necessary conformational changes of modular nonribosomal peptide synthetases. Curr Opin Chem Biol (Sep), 35: 89-96.
- Meneely KM, Sundlov JA, Gulick AM, Moran GR, Lamb AL. (2016) An Open and Shut Case: The Interaction of Magnesium with MST Enzymes. J Am Chem Soc (Jul), 138(29): 9277-9293.
- Bailey DC, Drake EJ, Grant TD, Gulick AM. (2016) Structural and Functional Characterization of Aerobactin Synthetase IucA from a Hypervirulent Pathotype of Klebsiella pneumoniae. Biochemistry (Jun), 55(25): 3559-3570.
- Richard JP, Amyes TL, Malabanan MM, Zhai X, Kim KJ, Reinhardt CJ, Wierenga RK, Drake EJ, Gulick AM. (2016) Structure-Function Studies of Hydrophobic Residues That Clamp a Basic Glutamate Side Chain during Catalysis by Triosephosphate Isomerase. Biochemistry (May), 55(21): 3036-3047.
- Drake EJ, Gulick AM. (2016) 1.2 Å resolution crystal structure of the periplasmic aminotransferase PvdN from Pseudomonas aeruginosa. Acta Crystallogr F Struct Biol Commun (May), 72(Pt 5): 403-408.
- Miller BR, Gulick AM. (2016) Structural Biology of Nonribosomal Peptide Synthetases. Methods Mol Biol (Jan), 1401: 3-29.
- Branchini BR, Behney CE, Southworth TL, Fontaine DM, Gulick AM, Vinyard DJ, Brudvig GW. (2015) Experimental Support for a Single Electron-Transfer Oxidation Mechanism in Firefly Bioluminescence. J Am Chem Soc (Jun), 137(24): 7592-7595.
- Zhu J, Lippa GM, Gulick AM, Tipton PA. (2015) Examining Reaction Specificity in PvcB, a Source of Diversity in Isonitrile-Containing Natural Products. Biochemistry (Apr), 54: 575-581.
- Mitchell CA, Tucker AC, Escalante-Semerena JC, Gulick AM. (2015) The structure of S. lividans acetoacetyl-CoA synthetase shows a novel interaction between the C-terminal extension and the N-terminal domain. Proteins (Mar), 83(3): 575-581.
- Kholodar SA, Allen CL, Gulick AM, Murkin AS. (2015) The Role of Phosphate in a Multistep Enzymatic Reaction: Reactions of the Substrate and Intermediate in Pieces. J Am Chem Soc (Feb).
- Russo TA, Olson R, Macdonald U, Metzger D, Maltese LM, Drake EJ, Gulick AM. (2014) Aerobactin Mediates Virulence and Accounts for Increased Siderophore Production under Iron-Limiting Conditions by Hypervirulent (Hypermucoviscous) Klebsiella pneumoniae. Infect Immun (Jun), 82(6): 2356-2367.
- Kholodar SA, Tombline G, Liu J, Tan Z, Allen CL, Gulick AM, Murkin AS. (2014) Alteration of the Flexible Loop in 1-Deoxy-d-xylulose-5-phosphate Reductoisomerase Boosts Enthalpy-Driven Inhibition by Fosmidomycin. Biochemistry (Jun), 53(21): 3423-3431.
- Miller BR, Sundlov JA, Drake EJ, Makin TA, Gulick AM. (2014) Analysis of the linker region joining the adenylation and carrier protein domains of the modular nonribosomal peptide synthetases. Proteins (Jun).
- Allen CL, Gulick AM. (2014) Structural and bioinformatic characterization of an Acinetobacter baumannii type II carrier protein. Acta Crystallogr D Biol Crystallogr (Jun), 70(Pt 6): 1718-1725.
- Wurst JM, Drake EJ, Theriault JR, Jewett IT, VerPlank L, Perez JR, Dandapani S, Palmer M, Moskowitz SM, Schreiber SL, Munoz B, Gulick AM. (2014) Identification of Inhibitors of PvdQ, an Enzyme Involved in the Synthesis of the Siderophore Pyoverdine. ACS Chem Biol (May).
- Demonte D, Drake EJ, Lim KH, Gulick AM, Park S. (2013) Structure-based engineering of streptavidin monomer with a reduced biotin dissociation rate. Proteins (Sep), 81(9): 1621-1633.
- Sundlov JA, Gulick AM. (2013) Structure determination of the functional domain interaction of a chimeric nonribosomal peptide synthetase from a challenging crystal with noncrystallographic translational symmetry. Acta Crystallogr D Biol Crystallogr (Aug), 69(Pt 8): 1482-1492.
- Demonte D, Drake EJ, Lim KH, Gulick AM, Park S. (2013) Structure based engineering of streptavidin monomer with a reduced biotin dissociation rate. Proteins (May).
- Neres J, Engelhart CA, Drake EJ, Wilson DJ, Fu P, Boshoff HI, Barry CE, Gulick AM, Aldrich CC. (2013) Non-Nucleoside Inhibitors of BasE, an Adenylating Enzyme in the Siderophore Biosynthetic Pathway of the Opportunistic Pathogen Acinetobacter baumannii. J Med Chem (Mar), 56(6): 2385-2405.
- Tessier MB, Grant OC, Heimburg-Molinaro J, Smith D, Jadey S, Gulick AM, Glushka J, Deutscher SL, Rittenhouse-Olson K, Woods RJ. (2013) Computational Screening of the Human TF-Glycome Provides a Structural Definition for the Specificity of Anti-Tumor Antibody JAA-F11. PLoS One (Jan), 8(1): 54874-54874.
- Wilson DJ, Shi C, Teitelbaum AM, Gulick AM, Aldrich CC. (2013) Characterization of AusA: A Dimodular Nonribosomal Peptide Synthetase Responsible for the Production of Aureusimine Pyrazinones. Biochemistry (Jan).
- Muppidi A, Doi K, Edwardraja S, Drake EJ, Gulick AM, Wang HG, Lin Q. (2012) Rational design of proteolytically stable, cell-permeable peptide-based selective Mcl-1 inhibitors. J Am Chem Soc (Sep), 134(36): 14734-14737.
- Sundlov JA, Fontaine DM, Southworth TL, Branchini BR, Gulick AM. (2012) Crystal structure of firefly luciferase in a second catalytic conformation supports a domain alternation mechanism. Biochemistry (Aug), 51(33): 6493-6495.
- Mitchell CA, Shi C, Aldrich CC, Gulick AM. (2012) Structure of PA1221, a nonribosomal peptide synthetase containing adenylation and peptidyl carrier protein domains. Biochemistry (Apr), 51(15): 3252-3263.
- Sundlov JA, Shi C, Wilson DJ, Aldrich CC, Gulick AM. (2012) Structural and functional investigation of the intermolecular interaction between NRPS adenylation and carrier protein domains. Chem Biol (Feb), 19(2): 188-198.
- Drake EJ, Gulick AM. (2011) Structural characterization and high-throughput screening of inhibitors of PvdQ, an NTN hydrolase involved in pyoverdine synthesis. ACS Chem Biol (Nov), 6(11): 1277-1286.
- Drake EJ, Duckworth BP, Neres J, Aldrich CC, Gulick AM. (2010) Biochemical and structural characterization of bisubstrate inhibitors of BasE, the self-standing nonribosomal peptide synthetase adenylate-forming enzyme of acinetobactin synthesis. Biochemistry (Nov), 49(43): 9292-9305.
- Sundlov JA, Gulick AM. (2009) Insights into resistance against lincosamide antibiotics. Structure (Dec), 17(12): 1549-1550.
- Shah MB, Ingram-Smith C, Cooper LL, Qu J, Meng Y, Smith KS, Gulick AM. (2009) The 2.1 A crystal structure of an acyl-CoA synthetase from Methanosarcina acetivorans reveals an alternate acyl-binding pocket for small branched acyl substrates. Proteins (Nov), 77(3): 685-698.
- Gulick AM. (2009) Conformational dynamics in the Acyl-CoA synthetases, adenylation domains of non-ribosomal peptide synthetases, and firefly luciferase. ACS Chem Biol (Oct), 4(10): 811-827.
- Wu R, Reger AS, Lu X, Gulick AM, Dunaway-Mariano D. (2009) The mechanism of domain alternation in the acyl-adenylate forming ligase superfamily member 4-chlorobenzoate: coenzyme A ligase. Biochemistry (May), 48(19): 4115-4125.
- Gulick AM. (2009) Ironing out a new siderophore synthesis strategy. Nat Chem Biol (Mar), 5(3): 143-144.
- Drake EJ, Gulick AM. (2008) Three-dimensional structures of Pseudomonas aeruginosa PvcA and PvcB, two proteins involved in the synthesis of 2-isocyano-6,7-dihydroxycoumarin. J Mol Biol (Dec), 384(1): 193-205.
- Wu R, Cao J, Lu X, Reger AS, Gulick AM, Dunaway-Mariano D. (2008) Mechanism of 4-chlorobenzoate:coenzyme a ligase catalysis. Biochemistry (Aug), 47(31): 8026-8039.
- Reger AS, Wu R, Dunaway-Mariano D, Gulick AM. (2008) Structural characterization of a 140 degrees domain movement in the two-step reaction catalyzed by 4-chlorobenzoate:CoA ligase. Biochemistry (Aug), 47(31): 8016-8025.
- Wu R, Reger AS, Cao J, Gulick AM, Dunaway-Mariano D. (2007) Rational redesign of the 4-chlorobenzoate binding site of 4-chlorobenzoate: coenzyme a ligase for expanded substrate range. Biochemistry (Dec), 46(50): 14487-14499.
- Drake EJ, Cao J, Qu J, Shah MB, Straubinger RM, Gulick AM. (2007) The 1.8 A crystal structure of PA2412, an MbtH-like protein from the pyoverdine cluster of Pseudomonas aeruginosa. J. Biol. Chem (Jul), 282(28): 20425-20434.
- Reger AS, Carney JM, Gulick AM. (2007) Biochemical and crystallographic analysis of substrate binding and conformational changes in acetyl-CoA synthetase. Biochemistry (Jun), 46(22): 6536-6546.
- Sundlov JA, Garringer JA, Carney JM, Reger AS, Drake EJ, Duax WL, Gulick AM. (2006) Determination of the crystal structure of EntA, a 2,3-dihydro-2,3-dihydroxybenzoic acid dehydrogenase from Escherichia coli. Acta Crystallogr D Biol Crystallogr (Jul), 62(Pt 7): 734-740.
- Drake EJ, Nicolai DA, Gulick AM. (2006) Structure of the EntB multidomain nonribosomal peptide synthetase and functional analysis of its interaction with the EntE adenylation domain. Chem Biol (Apr), 13(4): 409-419.
- Wang Y, Feng L, Said M, Balderman S, Fayazi Z, Liu Y, Ghosh D, Gulick AM. (2005) Analysis of the 2.0 A crystal structure of the protein-DNA complex of the human PDEF Ets domain bound to the prostate specific antigen regulatory site. Biochemistry (May), 44(19): 7095-7106.
- Gulick AM, Lu X, Dunaway-Mariano D. (2004) Crystal structure of 4-chlorobenzoate:CoA ligase/synthetase in the unliganded and aryl substrate-bound states. Biochemistry (Jul), 43(27): 8670-8679.
- Gulick AM, Starai VJ, Horswill AR, Homick KM, Escalante-Semerena JC. (2003) The 1.75 A crystal structure of acetyl-CoA synthetase bound to adenosine-5'-propylphosphate and coenzyme A. Biochemistry (Mar), 42(10): 2866-2873.
- Gulick AM, Horswill AR, Thoden JB, Escalante-Semerena JC, Rayment I. (2002) Pentaerythritol propoxylate: a new crystallization agent and cryoprotectant induces crystal growth of 2-methylcitrate dehydratase. Acta Crystallogr D Biol Crystallogr (Feb), 58(Pt 2): 306-309.
- Gulick AM, Schmidt DM, Gerlt JA, Rayment I. (2001) Evolution of enzymatic activities in the enolase superfamily: crystal structures of the L-Ala-D/L-Glu epimerases from Escherichia coli and Bacillus subtilis. Biochemistry (Dec), 40(51): 15716-15724.
- Gulick AM, Hubbard BK, Gerlt JA, Rayment I. (2001) Evolution of enzymatic activities in the enolase superfamily: identification of the general acid catalyst in the active site of D-glucarate dehydratase from Escherichia coli. Biochemistry (Aug), 40(34): 10054-10062.
- Gulick AM, Hubbard BK, Gerlt JA, Rayment I. (2000) Evolution of enzymatic activities in the enolase superfamily: crystallographic and mutagenesis studies of the reaction catalyzed by D-glucarate dehydratase from Escherichia coli. Biochemistry (Apr), 39(16): 4590-4602.
- Gulick AM, Bauer CB, Thoden JB, Pate E, Yount RG, Rayment I. (2000) X-ray structures of the Dictyostelium discoideum myosin motor domain with six non-nucleotide analogs. J Biol Chem (Jan), 275(1): 398-408.
- Gulick AM, Palmer DR, Babbitt PC, Gerlt JA, Rayment I. (1998) Evolution of enzymatic activities in the enolase superfamily: crystal structure of (D)-glucarate dehydratase from Pseudomonas putida. Biochemistry (Oct), 37(41): 14358-14368.
- Gulick AM, Song H, Endow SA, Rayment I. (1998) X-ray crystal structure of the yeast Kar3 motor domain complexed with Mg.ADP to 2.3 A resolution. Biochemistry (Feb), 37(7): 1769-1776.
- Gulick AM, Bauer CB, Thoden JB, Rayment I. (1997) X-ray structures of the MgADP, MgATPgammaS, and MgAMPPNP complexes of the Dictyostelium discoideum myosin motor domain. Biochemistry (Sep), 36(39): 11619-11628.
- Thoden JB, Gulick AM, Holden HM. (1997) Molecular structures of the S124A, S124T, and S124V site-directed mutants of UDP-galactose 4-epimerase from Escherichia coli. Biochemistry (Sep), 36(35): 10685-10695.
- Liu Y, Thoden JB, Kim J, Berger E, Gulick AM, Ruzicka FJ, Holden HM, Frey PA. (1997) Mechanistic roles of tyrosine 149 and serine 124 in UDP-galactose 4-epimerase from Escherichia coli. Biochemistry (Sep), 36(35): 10675-10684.
- Manoharan HT, Gallo J, Gulick AM, Fahl WE. (1997) High-level production and purification of biologically active proteins from bacterial and mammalian cells using the tandem pGFLEX expression system. Gene (Jul), 193(2): 229-237.
- Gulick AM, Rayment I. (1997) Structural studies on myosin II: communication between distant protein domains. Bioessays (Jul), 19(7): 561-569.
- Gulick AM, Fahl WE. (1995) Forced evolution of glutathione S-transferase to create a more efficient drug detoxication enzyme. Proc Natl Acad Sci U S A (Aug), 92(18): 8140-8144.
- Gulick AM, Fahl WE. (1995) Mammalian glutathione S-transferase: regulation of an enzyme system to achieve chemotherapeutic efficacy. Pharmacol Ther (May), 66(2): 237-257.
- Gulick AM, Goihl AL, Fahl WE. (1992) Structural studies on human glutathione S-transferase pi. Family of native-specific monoclonal antibodies used to block catalysis. J Biol Chem (Sep), 267(26): 18946-18952.
- Manoharan TH, Gulick AM, Puchalski RB, Servais AL, Fahl WE. (1992) Structural studies on human glutathione S-transferase pi. Substitution mutations to determine amino acids necessary for binding glutathione. J Biol Chem (Sep), 267(26): 18940-18945.
- Manoharan TH, Gulick AM, Reinemer P, Dirr HW, Huber R, Fahl WE. (1992) Mutational substitution of residues implicated by crystal structure in binding the substrate glutathione to human glutathione S-transferase pi. J Mol Biol (Jul), 226(2): 319-322.
- Manoharan TH, Welch PJ, Gulick AM, Puchalski RB, Lathrop AL, Fahl WE. (1991) Expression of tandem glutathione S-transferase recombinant genes in COS cells for analysis of efficiency of protein expression and associated drug resistance. Mol Pharmacol (Apr), 39(4): 461-467.
Professional Memberships:
- American Society for Biochemistry and Molecular Biology (2018–present)
- American Crystallographic Association (2000–2016)
- American Chemical Society (1996–present)
- American Association for the Advancement of Science (1992–present)
Presentations:
- "Structural Studies into the Biosynthesis of Bacterial Natural Products." Invited Seminar (2022)
- "Structural Studies into the Biosynthesis of Bacterial Natural Products." Invited Seminar. Department of Pharmacology and Toxicology, University at Buffalo (2021)
- "Nonribosomal Peptide Biosynthesis: Studies of a Protein Assembly Line." Invited Seminar, Department of Biology, University at Buffalo (2020)
- "Structural Studies of Antibiotic Production by Nonribosomal Peptide Synthetases" Invited Seminar, Department of Biochemistry, University of Illinois (2019)
- "Iron Acquisition in Bacterial Pathogens: Structural and Functional Studies of Siderophore Biosynthesis" Invited Seminar, Dalhousie University (2018)
- "The Structural Cycle of Nonribosomal Peptide Synthetases." American Society of Pharmacognosy Annual Meeting (2018)
- "Conformational Changes that Drive the Dynamic Structural Cycle of the Nonribosomal Peptide Synthetases" Directing Biosynthesis V Conference (2017)
Service Activities:
- Deutsche Forschungsgemeinschaft; Ad hoc Reviewer (2021)
- ZRG1 F04A (20) Fellowships Review: Chemistry, Biochemistry, and Biophysics A; Grant Review Panel (2020)
- ZRG1 F04A (20) Fellowships Review: Chemistry, Biochemistry, and Biophysics A; Grant Review Panel (2020)
- Department of Structural Biology; Director of Graduate Studies (2020–present)
- Research Foundation Flanders (Fonds voor Wetenschappelijk Onderzoek-Vlaanderen, FWO), European Research Foundation, Strasbourg, France; External Reviewer (2020)
- Co-director of NSIM program; co-Director, Natural Sciences Interdisciplinary Master's (2020–present)
- Mentor and guide first year PhD students through courses and rotations; Director, PhD Program in Biomedical Sciences (2020–present)
- NWO Talent Programme Veni, Dutch Research Council, The Hague, The Netherlands.; Grant Review External Reviewer (2020)
- Journal of Biological Chemistry; Editorial Board Member (2020–present)
- Swiss National Science Foundation, Bern Switzerland.; Grant Review External Member (2018)
- Marsden Fund, Royal Society Te Aparangi, New Zealand.; Grant Review External Member (2018)
- Boeringer Ingelheim Fonds, Mainz, Germany.; Grant Review External Member (2017)
- National Science Foundation. Chemistry of Life Processes CAREER Proposals; Grant Review Panel Member (2017)