Mouhamed Awayda
Mouhamed Awayda
Professional Summary:
We study the regulation of ion transport in epithelia. We are interested in a sodium channel expressed in many epithelia throughout the body. In the kidneys, this channel and its regulation, modify renal sodium excretion and body sodium balance. We are interested in mechanisms which control channel activity and more specifically, in the mechanism of channel activation from the extracellular space. Our lab is also interested in the translational aspect of channel activation and specifically in the discovery of biomarkers of channel activators. These biomarkers can likely report on channel activation in vivo and in this case, can serve can predictors of human hypertension
Education and Training:
- PhD, University of Illinois at Urbana-Champaign
Journal Articles:
- Awayda MS, Van Driessche W, Helman SI, Awayda M. (1999) Frequency-dependent capacitance of the apical membrane of frog skin: dielectric relaxation processes. Biophys J (Jan), 76(1 Pt): 219-232.
- Awayda MS, Subramanyam M, Awayda M. (1998) Regulation of the epithelial Na+ channel by membrane tension. J Gen Physiol (Aug), 112(2): 97-111.
- Awayda MS, Tousson A, Benos DJ, Awayda M. (1997) Regulation of a cloned epithelial Na+ channel by its beta- and gamma-subunits. Am J Physiol (Dec), 273(6 Pt): 1889-1899.
- Ismailov II, Berdiev BK, Bradford AL, Awayda MS, Fuller CM, Benos DJ, Awayda M. (1996) Associated proteins and renal epithelial Na+ channel function. J Membr Biol (Jan), 149(2): 123-132.
- Awayda MS, Ismailov II, Berdiev BK, Fuller CM, Benos DJ, Awayda M. (1996) Protein kinase regulation of a cloned epithelial Na+ channel. J Gen Physiol (Jul), 108(1): 49-65.
- Benos DJ, Awayda MS, Berdiev BK, Bradford AL, Fuller CM, Senyk O, Ismailov II, Awayda M. (1996) Diversity and regulation of amiloride-sensitive Na+ channels. Kidney Int (Jun), 49(6): 1632-1637.
- Awayda M, Kumar S, Rappaport N. (1996) Relapsing group B streptococcal infection. Ann Intern Med (Aug), 125(4): 350-350.
- Ismailov II, Awayda MS, Jovov B, Berdiev BK, Fuller CM, Dedman JR, Kaetzel M, Benos DJ, Awayda M. (1996) Regulation of epithelial sodium channels by the cystic fibrosis transmembrane conductance regulator. J Biol Chem (Mar), 271(9): 4725-4732.
- Ismailov II, Awayda MS, Berdiev BK, Bubien JK, Lucas JE, Fuller CM, Benos DJ, Awayda M. (1996) Triple-barrel organization of ENaC, a cloned epithelial Na+ channel. J Biol Chem (Jan), 271(2): 807-816.
- Cunningham SA, Awayda MS, Bubien JK, Ismailov II, Arrate MP, Berdiev BK, Benos DJ, Fuller CM, Awayda M. (1995) Cloning of an epithelial chloride channel from bovine trachea. J Biol Chem (Dec), 270(52): 31016-31026.
- Benos DJ, Awayda MS, Ismailov II, Johnson JP, Awayda M. (1995) Structure and function of amiloride-sensitive Na+ channels. J Membr Biol (Jan), 143(1): 1-18.
- Awayda MS, Ismailov II, Berdiev BK, Benos DJ, Awayda M. (1995) A cloned renal epithelial Na+ channel protein displays stretch activation in planar lipid bilayers. Am J Physiol (Jun), 268(6 Pt): 1450-1459.
- Fuller CM, Awayda MS, Arrate MP, Bradford AL, Morris RG, Canessa CM, Rossier BC, Benos DJ, Awayda M. (1995) Cloning of a bovine renal epithelial Na+ channel subunit. Am J Physiol (Sep), 269(3 Pt): 641-654.
- Kidder GW, Awayda MS, Awayda M. (1989) Effects of azide on gastric mucose. Biochim Biophys Acta (Jan), 973(1): 59-66.
- Gondzik V, Awayda MS, Awayda M. (2011) Methods for stable recording of short-circuit current in a Na+-transporting epithelium. Am J Physiol Cell Physiol (Jul), 301(1): 162-170.
- Awayda MS, Awayda KL, Pochynyuk O, Bugaj V, Stockand JD, Ortiz RM, Awayda M. (2011) Acute cholesterol-induced anti-natriuretic effects: role of epithelial Na+ channel activity, protein levels, and processing. J Biol Chem (Jan), 286(3): 1683-1695.
- Hu JC, Bengrine A, Lis A, Awayda MS, Awayda M. (2009) Alternative mechanism of activation of the epithelial na+ channel by cleavage. J Biol Chem (Dec), 284(52): 36334-36345.
- Kopkan L, Khan MA, Lis A, Awayda MS, Majid DS, Awayda M. (2009) Cholesterol induces renal vasoconstriction and anti-natriuresis by inhibiting nitric oxide production in anesthetized rats. Am J Physiol Renal Physiol (Dec), 297(6): 1606-1613.
- Bengrine A, Li J, Hamm LL, Awayda MS, Awayda M. (2007) Indirect activation of the epithelial Na+ channel by trypsin. J Biol Chem (Sep), 282(37): 26884-26896.
- Ortiz RM, Graciano ML, Seth D, Awayda MS, Navar LG, Awayda M. (2007) Aldosterone receptor antagonism exacerbates intrarenal angiotensin II augmentation in ANG II-dependent hypertension. Am J Physiol Renal Physiol (Jul), 293(1): 139-147.
- Bengrine A, Li J, Awayda MS, Awayda M. (2007) The A-kinase anchoring protein 15 regulates feedback inhibition of the epithelial Na+ channel. FASEB J (Apr), 21(4): 1189-1201.
- Qu YJ, Bondarenko VE, Xie C, Wang S, Awayda MS, Strauss HC, Morales MJ. (2007) W-7 modulates Kv4.3: pore block and Ca2+-calmodulin inhibition. Am J Physiol Heart Circ Physiol (May), 292(5): 2364-2377.
- Awayda MS, Shao W, Vukojicic I, Bengrine A, Awayda M. (2006) A simple in vivo method for assessing changes of membrane-bound ion channel density in Xenopus oocytes. Methods Mol Biol (Jan), 337: 101-115.
- Tobey NA, Argote CM, Awayda MS, Vanegas XC, Orlando RC, Awayda M. (2007) Effect of luminal acidity on the apical cation channel in rabbit esophageal epithelium. Am J Physiol Gastrointest Liver Physiol (Mar), 292(3): 796-805.
- Tobey NA, Argote CM, Awayda MS, Vanegas XC, Orlando RC, Awayda M. (2006) EFFECT OF LUMINAL ACIDITY ON THE APICAL CATION CHANNEL IN RABBIT ESOPHAGEAL EPITHELIUM. Am J Physiol Gastrointest Liver Physiol (Apr).
- Fuller AJ, Hauschild BC, Gonzalez-Villalobos R, Awayda MS, Imig JD, Inscho EW, Navar LG, Awayda M. (2005) Calcium and chloride channel activation by angiotensin II-AT1 receptors in preglomerular vascular smooth muscle cells. Am J Physiol Renal Physiol (Oct), 289(4): 760-767.
- Shao W, Orlando RC, Awayda MS, Awayda M. (2005) Bisphosphonates stimulate an endogenous nonselective cation channel in Xenopus oocytes: potential mechanism of action. Am J Physiol Cell Physiol (Aug), 289(2): 248-256.
- Awayda MS, Bengrine A, Tobey NA, Stockand JD, Orlando RC, Awayda M. (2004) Nonselective cation transport in native esophageal epithelia. Am J Physiol Cell Physiol (Aug), 287(2): 395-402.
- Awayda MS, Shao W, Guo F, Zeidel M, Hill WG, Awayda M. (2004) ENaC-membrane interactions: regulation of channel activity by membrane order. J Gen Physiol (Jun), 123(6): 709-727.
- Tobey NA, Hosseini SS, Argote CM, Dobrucali AM, Awayda MS, Orlando RC, Awayda M. (2004) Dilated intercellular spaces and shunt permeability in nonerosive acid-damaged esophageal epithelium. Am J Gastroenterol (Jan), 99(1): 13-22.
- Awayda MS, Platzer JD, Reger RL, Bengrine A, Awayda M. (2002) Role of PKCalpha in feedback regulation of Na(+) transport in an electrically tight epithelium. Am J Physiol Cell Physiol (Oct), 283(4): 1122-1132.
- Dobrucali A, Tobey NA, Awayda MS, Argote C, Abdulnour-Nakhoul S, Shao W, Orlando RC, Awayda M. (2002) Physiological and morphological effects of alendronate on rabbit esophageal epithelium. Am J Physiol Gastrointest Liver Physiol (Sep), 283(3): 576-586.
- Segal A, Awayda MS, Eggermont J, Van Driessche W, Weber WM, Awayda M. (2002) Influence of voltage and extracellular Na(+) on amiloride block and transport kinetics of rat epithelial Na(+) channel expressed in Xenopus oocytes. Pflugers Arch (Mar), 443(5-6): 882-891.
- Awayda MS, Boudreaux MJ, Reger RL, Hamm LL, Awayda M. (2000) Regulation of the epithelial Na(+) channel by extracellular acidification. Am J Physiol Cell Physiol (Dec), 279(6): 1896-1905.
- Awayda MS, Awayda M. (2000) Specific and nonspecific effects of protein kinase C on the epithelial Na (+) channel. J Gen Physiol (May), 115(5): 559-570.
- Awayda MS, Awayda M. (1999) Regulation of the epithelial Na(+) channel by intracellular Na(+). Am J Physiol (Aug), 277(2 Pt): 216-224.