Robert H. Blessing PhD
Robert H. Blessing
PhD
Professional Summary:
Professional Interests and Activities:
Teaching and research in structural molecular biology, biochemistry, and biophysics. Experimental, computational, and theoretical methods of biocrystallography.
Education and Training:
- PhD, Chemistry, Ohio University (1970)
- BS, Chemistry, King‘s College (1962)
Employment:
- Professor of Structural Biology, Structural Biology, University at Buffalo Jacobs School of Medicine & Biomedical Sciences (2001-present)
- Reserach Professor of Chemistry, Chemistry, University at Buffalo Faculty of Natural Sciences and Mathematics (1991-present)
- Senior Research Scientist, Hauptman-Woodward Biomedical Research Institute, Inc. (1978-present)
- Associate Professor of Chemistry, Department of Chemistry and Physics, Mercyhurst College Mercyhurst College (1974–1978)
- Postdoctoral Research Associate in Crystallography, Chemistry, University at Buffalo Faculty of Natural Sciences and Mathematics (1972–1974)
- Postdoctoral Research Associate in Biocrystallography, Crystallography, University of Pittsburgh University Of Pittsburgh (1970–1972)
- Postdoctoral Fellow, Chemical Institute, University of Lund University of Lund (1969–1970)
Research Expertise:
- Crystallography
Journal Articles:
- Smith GD, Nagar B, Rini JM, Hauptman HA, Blessing RH. (1998) The use of SnB to determine an anomalous scattering substructure. Acta Crystallogr D Biol Crystallogr (Sep), 54(Pt 5): 799-804.
- Hu SH, Loughnan M, Miller R, Weeks CM, Blessing RH, Alewood PF, Lewis RJ, Martin JL. (1998) The 1.1 A resolution crystal structure of [Tyr15]EpI, a novel alpha-conotoxin from Conus episcopatus, solved by direct methods. Biochemistry (Aug), 37(33): 11425-11433.
- Guo DY, Blessing RH, Langs DA, Hauptman HA. (1997) On integrating the techniques of direct methods with anomalous dispersion. IV. A simplified perturbation treatment for SAS phasing. Acta Crystallogr A (Jan), 53 (: 74-83.
- Souhassou M, Schaber PM, Blessing RH. (1996) Structure of tris(cyclohexylammonium) phosphoenolpyruvate monohydrate and crystal chemistry of phosphoenolpyruvates. Acta Crystallogr B (Oct), 52 (: 865-875.
- Maniukiewicz W, Kwiatkowski W, Blessing RH. (1996) O-phospho-DL-threonine and O-phospho-L-threonine compared with their serine analogs. Acta Crystallogr C (Jul), 52 (: 1736-1741.
- Wallet JC, Cody V, Wojtczak A, Blessing RH. (1993) Structural and conformational studies on bio-active flavonoids. Crystal and molecular structure of a complex formed between 2‘,6‘-dimethoxyflavone and orthophosphoric acid: a model for flavone-nucleotide interactions. Anticancer Drug Des (Oct), 8(5): 325-332.
- Guo DY, Blessing RH, Hauptman HA. (1994) On integrating the techniques of direct methods with anomalous dispersion. III. Estimation of two-wavelength two-phase structure invariants. Acta Crystallogr A (May), 50 (: 307-311.
- Blessing RH. (1995) An empirical correction for absorption anisotropy. Acta Crystallogr A (Jan), 51 (: 33-38.
- Blessing RH. (1995) On the differences between X-ray and neutron thermal vibration parameters. Acta Crystallogr B (Oct), 51 (: 816-823.
- Souhassou M, Espinosa E, Lecomte C, Blessing RH. (1995) Experimental electron density in crystalline H3PO4. Acta Crystallogr B (Oct), 51 (: 661-668.
- Moss GR, Souhassou M, Blessing RH, Espinosa E, Lecomte C. (1995) Computational studies of crystalline H3PO4. Acta Crystallogr B (Oct), 51 (: 650-660.
- DeTitta GT, Parthasarathy R, Blessing RH, Stallings W. (1980) Carboxybiotin translocation mechanisms suggested by diffraction studies of biotin and its vitamers. Proc Natl Acad Sci U S A (Jan), 77(1): 333-337.
- Blessing RH. (1988) New analysis of the neutron diffraction data for anhydrous orthophosphoric acid and the structure of H3PO4 molecules in crystals. Acta Crystallogr B (Aug), 44 (: 334-340.
- Blessing RH, Langs DA. (1988) A priori estimation of scale and overall anisotropic temperature factors from the Patterson origin peak. Acta Crystallogr A (Sep), 44 (: 729-735.
- Guo DY, Blessing RH, Hauptman HA. (1991) On integrating the techniques of direct methods with anomalous dispersion. II. Statistical properties of the two-phase structure invariants. Acta Crystallogr A (Jul), 47 (: 340-345.
- Souhassou M, Lecomte C, Blessing RH, Aubry A, Rohmer MM, Wiest R, Bénard M, Marraud M. (1991) Electron distributions in peptides and related molecules. 1. An experimental and theoretical study of N-acetyl-L-tryptophan methylamide. Acta Crystallogr B (Apr), 47 (: 253-266.
- Smith GD, Pangborn WA, Blessing RH. (2005) The structure of T6 bovine insulin. Acta Crystallogr D Biol Crystallogr (Nov), 61(Pt 11): 1476-1482.
- Smith GD, Blessing RH, Ealick SE, Fontecilla-Camps JC, Hauptman HA, Housset D, Langs DA, Miller R. (1997) Ab initio structure determination and refinement of a scorpion protein toxin. Acta Crystallogr D Biol Crystallogr (Sep), 53(Pt 5): 551-557.
- Blessing RH, Guo DY, Langs DA. (1996) Statistical expectation value of the Debye-Waller factor and E(hkl) values for macromolecular crystals. Acta Crystallogr D Biol Crystallogr (Mar), 52(Pt 2): 257-266.
- Weeks CM, Hauptman HA, Smith GD, Blessing RH, Teeter MM. (1995) Crambin: a direct solution for a 400-atom structure. Acta Crystallogr D Biol Crystallogr (Jan), 51(Pt 1): 33-38.
- Langs DA, Blessing RH, Duax WL. (1992) Crystal structure of valinomycin-monohydrate cage complexes crystallized from dioxane. Int J Pept Protein Res (Apr), 39(4): 291-299.
- Souhassou M, Lecomte C, Blessing RH. (1992) Crystal chemistry of Mg2P2O7.nH2O, n = 0, 2 and 6: magnesium-oxygen coordination and pyrophosphate ligation and conformation. Acta Crystallogr B (Aug), 48 (: 370-376.
- Smith GD, Blessing RH. (2003) Lessons from an aged, dried crystal of T(6) human insulin. Acta Crystallogr D Biol Crystallogr (Aug), 59(Pt 8): 1384-1394.
- Smith GD, Pangborn WA, Blessing RH. (2003) The structure of T6 human insulin at 1.0 A resolution. Acta Crystallogr D Biol Crystallogr (Mar), 59(Pt 3): 474-482.
- Hauptman HA, Guo DY, Xu H, Blessing RH. (2002) Algebraic direct methods for few-atoms structure models. Acta Crystallogr A (Jul), 58(Pt 4): 361-369.
- Langs DA, Blessing RH, Guo D. (2001) Map self-validation: improved criteria to resolve the SIR or SAS phase ambiguity. Acta Crystallogr D Biol Crystallogr (Sep), 57(Pt 9): 1351-1353.
- Smith GD, Pangborn WA, Blessing RH. (2001) Phase changes in T(3)R(3)(f) human insulin: temperature or pressure induced?. Acta Crystallogr D Biol Crystallogr (Aug), 57(Pt 8): 1091-1100.
- Langs DA, Blessing RH, Guo D. (2001) Improvement of SAS triple invariant estimates for macromolecular direct-methods phasing. Acta Crystallogr A (May), 57(Pt 3): 240-243.
- Langs DA, Blessing RH, Guo D. (2001) Map self-validation: a useful discriminator of phase correctness at low resolution. Acta Crystallogr D Biol Crystallogr (Apr), 57(Pt 4): 574-578.
- Von Dreele RB, Stephens PW, Smith GD, Blessing RH. (2000) The first protein crystal structure determined from high-resolution X-ray powder diffraction data: a variant of T3R3 human insulin-zinc complex produced by grinding. Acta Crystallogr D Biol Crystallogr (Dec), 56(Pt 12): 1549-1553.
- Smith GD, Ciszak E, Magrum LA, Pangborn WA, Blessing RH. (2000) R6 hexameric insulin complexed with m-cresol or resorcinol. Acta Crystallogr D Biol Crystallogr (Dec), 56(Pt 12): 1541-1548.
- Guo DY, Blessing RH, Langs DA. (2000) Globbic approximation in low-resolution direct-methods phasing. Acta Crystallogr D Biol Crystallogr (Sep), 56(Pt 9): 1148-1155.
- Howell PL, Blessing RH, Smith GD, Weeks CM. (2000) Optimizing DREAR and SnB parameters for determining Se-atom substructures. Acta Crystallogr D Biol Crystallogr (May), 56(Pt 5): 604-617.
- Guo DY, Blessing RH, Langs DA. (2000) Bulk-solvent correction in direct-methods phasing. Acta Crystallogr D Biol Crystallogr (Apr), 56(Pt 4): 451-457.
- Jelsch C, Teeter MM, Lamzin V, Pichon-Pesme V, Blessing RH, Lecomte C. (2000) Accurate protein crystallography at ultra-high resolution: valence electron distribution in crambin. Proc Natl Acad Sci U S A (Mar), 97(7): 3171-3176.
- Langs DA, Blessing RH, Guo D. (1999) Progress on the direct-methods solution of macromolecular structures using single-wavelength anomalous-dispersion (SAS) data. Acta Crystallogr A (Jul), 55 (: 755-760.
- Guo DY, Blessing RH, Langs DA, Smith GD. (1999) On 'globbicity' of low-resolution protein structures. Acta Crystallogr D Biol Crystallogr (Jan), 55(Pt 1): 230-237.