We help investigators generate induced pluripotent stem cells from human skin fibroblasts using lentiviral vectors delivering five reprogramming factors (Oct4, Sox2, Klf4, c-Myc and Nanog).
The end user is responsible for providing primary skin fibroblasts at an early passage.
We reprogram the cells to iPSC and perform characterization by PCR and immunostaining, and return the iPSC lines to you.
Variations of service may be developed on a collaborative basis, such as using cells from other species or other cell types. Interested investigators should contact the facility director for technical details.
We also train users to develop iPSC lines for basic and translational research.
Functions of this facility include: