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Core Facilities

Next Generation Sequencing and Expression

Genomic core

The Next Generation Sequencing and Expression facility offers instrumentation and services — including gene expression and genome analysis — that support a variety of different experiments.

Services

This facility offers high-throughput, next-generation sequencing. We also provide gene expression analysis services on Affymetrix platforms, including 3’-expression, miRNA, exon and gene arrays.

Gene Expression

Our Affymetrix Gene Chip System provides expression analysis services on all Affymetrix Gene Chips, including 3’-expression, miRNA, exon and gene arrays.

It performs sample quality analysis using the Agilent 2100 Bioanalyzer, target labeling, fragmentation, hybridization, staining and scanning.

Lab personnel conduct initial data analysis. You can arrange additional data analysis services with bioinformatics specialists at the University at Buffalo or Roswell Park Cancer Institute.

Genome Analysis

Our illumina HiSeq 2500 platform supports a variety of different experiments, including whole-genome and candidate-region resequencing, transcriptome analysis, small RNA discovery, methylation profiling and protein-nucleic acid interaction analysis at a genome-wide scale. It is an excellent platform for projects that need broad coverage and can accommodate short read lengths.

Instrumentation

  • Affymetrix Gene Chip System comprising a GeneChip Scanner 3000, two Fluidics 450 units and a GeneChip Hybridization Oven 640
  • illumina HiSeq 2500, a flexible and scalable platform that features two run modes, rapid run and high output run mode and the ability to process one or two flow cells simultaneously
  • illumina MiSeq, a platform specializing in more targeted sequencing geared toward smaller genomes and smaller search space investigation

Location

UB New York State Center of Excellence in Bioinformatics and Life Sciences
Buffalo Niagara Medical Campus
701 Ellicott Street
Buffalo, NY 14203

Contact Information

Director

Nowak, Norma

Norma Nowak, PhD

Professor

701 Ellicott Street, Room B2-201 Ellicott Street, Center of Excellence Buffalo, NY 14203

Phone: 881-8903; 845-1698

Email: njnowak@buffalo.edu

Laboratory Manager

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Sujith A. Valiyaparambil

Center of Excellence in Bioinformatics and Life Sciences Lab #B3-123

701 Ellicott Street, Buffalo, NY 14203

Phone: (716) 881-7514

Email: alphy@buffalo.edu