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John                           Panepinto

John C. Panepinto PhD

Department of Microbiology and Immunology

Associate Professor

Specialty/Research Focus

Eukaryotic Pathogenesis; Gene Expression; Infectious Disease; Microbial Pathogenesis; Microbiology; RNA; Signal Transduction

 
Professional Summary:

There are estimated to be over one million species of fungi on the earth, yet very few of these species are capable of causing deadly systemic infections in humans. One of the major limiting factors for most fungi is their inability to grow at mammalian core body temperature. We utilize the fungal pathogen Cryptococcus neoformans var. grubii as representative fungal pathogen to understand how these few fungi have adapted to growth at mammalian body temperature. C. neoformans is a worthy pathogen as it is estimated to cause over 500,000 deaths from meningoencephalitis per year, primarily in Africa and Southeast Asia as an HIV/AIDS comorbidity. We are able to utilize the temperature limited Cryptococcus amylolentus, an environmental strain that produces similar virulence factors to C. neoformans and is fully virulent in surrogate invertebrate hosts at permissive temperatures, as a comparator.
We have discovered that host temperature adaptation in C. neoformans is accompanied by a re-programming of gene expression at the level of mRNA stability. In response to temperature stress, C. neoformans rapidly degrades mRNAs encoding energy consuming machinery such as ribosomes, and prioritizes the translation of stress-responsive mRNAs on existing ribosomes. Because mRNA synthesis and decay are coupled processes, we are seeking to identify the protein components of mRNA complexes that mediate the specificity of this decay process, and post translational modifications, such as arginine methylation and phosphorylation, that modify their function. In addition, we are investigating the signaling pathways that accelerate or slow mRNA decay in response to specific environmental stimuli such as host temperature and nutrient deprivation.
Finally, mRNA decay not only alters gene expression at the post-transcriptional level, but the degradation of abundant mRNAs during stress releases nucleotide intermediates that can be utilized by the stressed cell to promote genome stability. Our work is investigating both the process of mRNA degradation as well as nucleotide metabolic pathways as drug targets in C. neoformans and other fungal pathogens.

Education and Training:
  • PhD, Pathobiology and Molecular Medicine, University of Cincinnati (2002)
  • BS, Biology (Microbiology and Immunology), Virginia Tech (1997)
  • BS, Biochemistry, Virginia Tech (1997)
Employment:
  • Associate Professor, University at Buffalo, the State University of New York (2014-present)
  • Assistant Professor, Microbiology and Immunology, University at Buffalo, the State University of New York (2008–2014)
  • Postdoctoral Research Associate, Section of Infectious Diseases, Department of Medicine, University of Illinois at Chicago (2003–2008)
  • Postdoctoral Research Associate, Pathology and Laboratory Medicine, University of Cincinnati (2002–2003)
  • Adjunct Faculty, Biology, The College of Mount St. Joseph (2002)

Research Expertise:
  • Fungal Pathogenesis
  • Mycology
Research Centers:
  • Witebsky Center for Microbial Pathogenesis and Immunology
UB 2020 Strategic Strengths:
  • Molecular Recognition in Biological Systems and Bioinformatics
Grants and Sponsored Research:
  • July 2011–June 2016
    R01 AI089920-01A1 Stress Responsive RNA Regulons in Cryptococcus neoformans
    NIAID
    Role: Principal Investigator
    $1,718,870

Journal Articles:
See All (29 Total) >


Clinical Specialties:
Clinical Offices:
Insurance Accepted:

Contact Information

117 Biomedical Research Building
Buffalo, NY 14214
Phone: (716) 829-2090
Email: jcp25@buffalo.edu


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