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John                           Kolega

John Kolega PhD

Department of Pathology and Anatomical Sciences

Associate Professor / Graduate Program Director

Specialty/Research Focus

Cardiovascular Disease; Cytoskeleton and cell motility; Molecular and Cellular Biology; Molecular Basis of Disease

 
Professional Summary:

My primary research interest is the behavior of endothelial cells, which form the inner lining of blood vessels and are key players in the remodeling events that occur during wound healing, aneurysm formation, tumor growth, and a wide variety of disease conditions. There are two questions about endothelial behavior that drive most of the research in my laboratory:
(1) How does an endothelial cell migrate during wound healing and blood-vessel remodeling? We are particularly interested in the motor protein, myosin II, and how it exerts force within the cytoskeleton to push or pull the cell as it moves. In order to study the organization and movements of cytoskeletal proteins - and not just there biochemical properties - we use a variety of light microscopic methods to examine the dynamics and biochemistry of cytoskeletal proteins in living migrating endothelial cells. We also use conventional biochemical, genetic, and pharmacological manipulations to investigate the regulatory events that control myosin II behavior in situ.
(2) How do endothelial cells sense and respond to their mechanical environment?
Blood vessels remodel to accommodate long-term changes in blood flow. Certain flow environments can cause destructive remodeling that leads to cerebral aneurysms (local “ballooning” of vessels). Working with biomedical engineers in the laboratory of Dr. Hui Meng at the Toshiba Stroke Research Center, we use cell culture and whole animal systems to examine how endothelial cells respond to specific hemodynamic micro-environments in order to understand the mechanism and regulation of flow-induced remodeling, especially as it relates to cerebral aneurysms.
A third interest is understanding the response of cultured endothelial cells to electrical fields, which have been shown to orient endothelial migration in vitro and to suppress edema in vivo by enhancing the endothelial permeability barrier.

Education and Training:
  • PhD, Cell Biology, Yale University (1984)
  • MPhil, Biology, Yale University (1981)
  • BA, Life Sciences, Massachusetts Institute of Technology (1978)
  • BS, Chemistry, Massachusetts Institute of Technology (1978)
Employment:
  • Associate Professor, Pathology and Anatomical Sciences, Univeristy of Buffalo, State University of New York (2001-present)
  • Post Doctoral Fellow, Laboratory for Developmental Biology and Anomalies, National Institutes of Health, National Institute for Dental Research
  • Assistant Professor, Pathology and Anatomical Sciences, University of Buffalo, State University of New York (2000–2001)
  • Clinical Assistant Professor, Pathology and Anatomical Sciences, University of Buffalo, State University of New York (1998–2000)
  • Assistant Professor, Pathology and Anatomical Sciences, University of Buffalo, State University of New York (1993–1998)
  • Special Research Faculty, Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, - (1992–1993)
  • Post Doctoral Fellow, Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University (1989–1992)
  • Post Doctoral Fellow, Dermatology, New York University Medical Center, Medicine (1985–1989)
  • Technical Collaborator, -, Brookhaven National Laboratory, - (1978)
  • Technical Collaborator, -, Brookhaven National Laboratory, - (1977)
Awards and Honors:
  • Didaskalos Award (2011)

Research Expertise:
  • Cell Biology: Cytoskeletal regulation and mechanism in cell migration. Endothelial cell biology.
  • Cell Biology: Cytoskeleton and Cell Motility
  • Gross Anatomy: back and extremeties
  • Vascular Biology: endothelial signaling, vessel wall remodeling
Research Centers:
  • Toshiba Stroke Research Center
UB 2020 Strategic Strengths:
  • Molecular Recognition in Biological Systems and Bioinformatics
Grants and Sponsored Research:
  • February 2009–February 2014
    Hemodynamic Induction of Pathologic Remodeling Leading to Intracranial Aneurysms
    National Institutes of Health/National Institute of Neurological Disorder and Stroke
    Role: Co-Investigator
    $1,733,595
  • October 2006–July 2007
    Summer Research Institute in Biomedical Materials Science
    National Institutes of Health
    Role: Co-Investigator
    $641,554
  • January 2000–December 2002
    Controlling the Direction of Endothelial Migration during Angiogenesis: Cytoskeletal Mechanism and Regulation
    American Heart Association
    Role: Principal Investigator
    $164,923
  • February 1995–January 1998
    Assembly and Regulation of Myosin II in Endothelial Locomotion
    National Science Foundation
    Role: Principal Investigator
    $237,000
  • June 1995–May 1996
    Physical Interventions for Controlling Edema
    State University of New York at Buffalo Provost's Multidisciplinary Research Pilot Project Program
    Role: Co-Investigator
    $20,000
  • June 1995–December 1995
    Multidisciplinary Pilot Project Program:Physical Interventions for Controlling Edema
    Multidisciplinary Pilot Project Program
    Role: Co-Investigator
    $20,000

Journal Articles:
See all (39 more)
Books and Book Chapters:
  • K. Hahn, John Kolega, J. Montibeller, R. DeBiasio, P. Post, J. Myers, D. L. Taylor. Fluorescent analogs: optical biosensors of the chemical and molecular dynamics of macromolecules in living cells.. Fluorescent and Luminescent Probes for Biological activity: A Practical Guide to Technology for Quantitative Real-Time Analysis (W.T. Mason, ed.). 1999.
  • John Kolega, Taylor, D. L.. Regulation of actin and myosin II dymamics in living cells.. Ordering the Membrane-Cytoskeleton Trilayer (J.S. Morrow and M.S. Mooseker, eds.). 1991.
  • John Kolega. The cellular basis of epithelial morphogenesis.. Developemtnal Biology: An Ongoing Synthesis (L.W. Browder, ed.). 1986; 2.

Professional Memberships:
  • American Heart Association Council (2000–present)
  • The American Association for the Advancement of Science (1991–present)
  • American Society for Cell Biology (1991–present)
Service Activities:
  • Academic Calendar Committee, School of Dental Medicine; Academic Calendar Committee, School of Dental Medicine; Committee Member (2011–present)
  • Editorial Board, ISRN Vascular Medicine; Editorial Board, ISRN Vascular Medicine, 2011 - present; Editorial Board Member (2011–present)
  • Editorial Board, Open Journal of Biophysics, 2011 - present; Editorial Board, Open Journal of Biophysics, 2011 - present; Editorial Board Member (2011–present)
  • Curriculum Committee, School of Dental Medicine; Curriculum Committee, School of Dental Medicine; Committee Member (2010–present)
  • First Year Committee, School of Dental Medicine; First Year Committee, School of Dental Medicine; Committee Member (2009–present)
  • Phase I Committee, School of Medicine; Phase I Committee, School of Medicine; Committee Member (2009–present)
  • Editorial Board, The Open Enzyme Inhibition Journal; Editorial Boards: The Open Enzyme Inhibition Journal, 2009 - present;; Editorial Board Member (2009–present)
  • Executive Committee, Department of Pathology & Anatomical Sciences; Executive Committee, Department of Pathology & Anatomical Sciences; Committee Member (2002–present)
  • Director of Graduate Studies; Director of Gradute Studies for the Antomy and Cell Biology Masters and PhD programs; Director (2001–present)
  • journal peer review; ad hoc review for the following journals: Nature; Journal of Cell Biology; Molecular Biology of the Cell; FASEB Journal; Biophysical Journal; American Journal of Physiology; Journal of Vascular Research; Arteriosclerosis, Thrombosis and Vascular Biology; Cardiovascular Research; Cell Motility and the Cytoskeleton; Experimental Cell Research; Cell Biochemistry and Biophysics; Journal of Neurobiology; Journal of the Royal Society Interface; Cell and Molecular Life Sciences; Wound Repair and Regeneration; Neurosurgery; Acta Biomaterialia; Peer Reviewer (2000–present)

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Contact Information

Department of Pathology & Anatomical Sciences
206 Farber Hall
3435 Main Street
Buffalo, NY 14214
Phone: (716) 829-3527
Fax: (716) 829-2911
Email: kolega@buffalo.edu


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