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Michael                        Malkowski

Michael G. Malkowski PhD

Department of Structural Biology

Professor and Chair

Specialty/Research Focus

Genomics and proteomics; Protein Function and Structure; Proteins and metalloenzymes

 
Professional Summary:

The Malkowski Laboratory is focused on understanding the structure and function of integral membrane enzymes involved in the conversion of lipid precursors into potent bioactive signaling molecules. We utilize a myriad of methods and techniques to characterize these enzymes, including X-ray crystallography, electron spin resonance spectroscopy, protein chemistry, biochemistry, molecular biology, cell biology, and kinetics.

Education and Training:
  • PhD, Biochemistry, Wayne State University (1997)
  • BS, Chemistry, University of Detroit, Magna Cum Laude (1991)
Employment:
  • Professor, Structural Biology, University of Buffalo (2014-present)
  • Principal Research Scientist, Hauptman-Woodward Medical Research Institute (2012-present)
  • Assistant Professor, Structural Biology, University of Buffalo (2002–2014)
  • Senior Research Scientist, Hauptman-Woodward Medical Research Institute (2007–2012)
  • Research Scientist, Hauptman-Woodward Medical Research Institute (2001–2007)
  • NIH Postdoctoral Fellow, Biochemistry, Michigan State University (1997–2001)
Awards and Honors:
  • Eicosanoid Research Foundation Young Investigator Award (2011)
  • Arthritis Investigator Award, Arthritis Foundation (2005)
  • NRSA Individual Postdoctoral Fellowship, National Institutes of Health (1999)
  • NIH Predoctoral Training Fellowship, Wayne State University (1993)
  • Merck Index Award, Outstanding Chemistry Student, University of Detroit (1991)
  • Presidential Scholarship, University of Detroit, Detroit, MI (1987)

Research Expertise:
  • Fatty Acid Metabolism: Interested in membrane associated enzymes involved in the (di)oxygenation of fatty acid substrates. Current systems being studied in the lab include 1) cyclooxygenase-1 and -2 (COX-1 and COX-2); and 2) pathogen-inducible oxygenase (PIOX).
  • Protein Expression and Purification: Extensive technical expertise in: 1) the expression of prokaryotic and eukaryotic proteins in E. coli, S. cerevisiae, and insect cell expression hosts; 2) purification of soluble and membrane proteins using various modes of column chromatography.
  • Structural Biology of Membrane Proteins: Expertise in structure-function studies of membrane proteins, including 1) expression in insect cells, E. coli, and S. cerevisiae; 2) detergent solubilization; 3) purification; 4) pre-crystallization screening of protein-detergent complexes; and 5) high-throughput crystallization screening and optimization.
  • X-ray Crystallography: Expertise in all aspects of structure determination using x-ray crystallographic methods, including: 1) high-throughput crystallization screening and optimization; 2) cryopreservation; 3) in-house and synchrotron data collection; 4) phasing (MIR and MAD); and 5) refinement and model building.
UB 2020 Strategic Strengths:
  • Molecular Recognition in Biological Systems and Bioinformatics
Grants and Sponsored Research:
  • September 2017–August 2020
    Community Crystallization Resource for Biological Macromolecules
    National Institutes of Health/NIGMS
    Role: Principal Investigator
  • August 2015–July 2019
    Structure and Dynamics of Cyclooxygenase Catalysis and Inhibition
    NIH/NIGMS
    Role: Principal Investigator
  • April 2013–March 2017
    Characterization of Treponema pallidum Rare Outer Membrane Proteins
    National Institutes of Health/NIAID
    Role: Co-Investigator
  • July 2010–June 2015
    Multi-Level Optimization of Membrane Proteins for Crystallography
    NIGMS
    Role: Principal Investigator
  • September 2011–May 2015
    Catalysis by Prostaglandin Endoperoxide H Synthases
    NIGMS
    Role: Co-Investigator
  • January 2008–December 2012
    T. pallidum Membrane Immunogènes and Anti-Oxidant Defense
    National Institutes of Health/NIAID
    Role: Co-Investigator
  • February 2007–January 2012
    Structure Biology of Oxylipin Biosynthesis
    NIH/NIGMS
    Role: Principal Investigator

Journal Articles:
See all (39 more)

Professional Memberships:
  • American Society for Biochemistry and Molecular Biology (2009–present)
Presentations:
  • "New Insights into Cyclooxygenase Catalysis and Inhibition" Seminar Series, University of Connecticut, Department of Molecular Biology and Biochemistry (2016)
  • "New Perspectives in Cyclooxygenase Structure, Function, and Dynamics" Seminar Series, SUNY at Buffalo, Department of Oral Biology (2014)
  • "Investigating Cyclooxygenase-2 Catalysis and Inhibition in a Lipid Bilayer" Bioactive Lipids in Cancer, Inflammation, and Related Disease 13th International Conference, Eicosanoid Research Foundation (2013)
  • "Monotopic Membrane Enzymes: Structural Features that Govern Catalysis at the Membrane Interface" Advanced Photon Source User's Meeting, Argonne National Laboratory (2013)
  • "Do Plants Have COX? Structural Studies of a Cyclooxygenase-like alpha-dioxygenase from Arabidopsis" , Hospital for Sick Kids, Department of Molecular and Structural Function (2012)
  • "Flexibility Drives Substrate Promiscuity in Cyclooxygenase-2" CHESS User's Meeting, Cornell University (2012)
  • "Insights into the Oxygenation of Substrates by COX-2" , SUNY-IT, Department of Chemistry (2012)
  • "Molecular Features that Govern the Oxygenation of Substrates by COX-2" 14th Winter Eicosanoid Conference (2012)
  • "The Structure of alpha-Dioxygenase: a Case Study in the Use of Detergent Phase Boundaries to Crystallize Membrane Proteins" , Michigan State University, Department of Biochemistry (2011)
  • "The Who, What, and Why of Structural Biology" , University of Detroit-Mercy, Department of Chemistry (2011)
  • "Substrate Binding to Cyclooxygenase-2: Insight into Specificity and Catalysis" Bioactive Lipids in Cancer, Inflammation, and related Diseases 12th International Conference, Eicosanoid Research Foundation (2011)
  • "High-Throughput Crystallization Screening for the Structural Biology Community" American Crystallographic Association Annual Meeting, American Crystallographic Association (2011)
  • "Structural Studies of Fatty Acid Oxygenation by Membrane Bound Enzymes" The Institute for Molecular Diversity and Drug Design 13th Annual Symposium, University of Louisville (2011)
  • "Using Experimentally Derived Detergent Phase Boundaries to Facilitate Crystallization of Membrane Proteins" 13th International Conference on the Crystallization of Biomolecules (2010)
  • "Multi-Level Optimization of Membrane Proteins" PSI:Biology Kickoff Meeting, National Institutes of Health, National Institute for General Medical Sciences (2010)
  • "Just a Phase? Using Experimentally-derived Detergent Phase Boundaries to Facilitate Crystallization of Membrane Proteins" 40th Mid-Atlantic Crystallography Meeting (2010)
  • "Using Experimentally-Derived Detergent Phase Boundaries to Facilitate Crystallization of Membrane Proteins" , GlaxoSmithKline (2010)
  • "Then and Now: Structural Biology of Cyclooxygenase-2" , Vanderbilt University, Institute of Chemical Biology (2010)
  • "CHTSB: Technology Development Highlights" Protein Structure Initiative Annual Meeting, National Institutes of Health, National Institute of General Medical Sciences (2009)
  • "Molecular Determinants Governing Oxylipin Biosynthesis in Fatty Acid Oxygenases" , Johns Hopkins University, Department of Chemistry (2009)
  • "Recent Advances in Membrane Protein Crystallization: Determination and Application of Empirically-Derived Detergent Phase Boundaries for Crystallization" 2009 SSRL/LCLS User's Meeting, Stanford University (2009)
  • "Molecular Determinants Governing Fatty Acid Oxygenation in alpha-dioxygenases" Regulatory Oxylipins: an International Symposium (2009)
  • "Technology Development Efforts at the Center for High-Throughput Structural Biology" Protein Structure Initiative Annual Meeting, National Institutes of Health, National Institute of General Medical Sciences (2008)
  • "Using Detergent Phase Boundaries to Crystallize Membrane Proteins" Protein Structure Initiative 7th Bottlenecks Workshop, National Institutes of Health, National Institute of General Medical Sciences (2008)
  • "Technology Developments at the CHTSB" Protein Structure Initiative Annual Meeting, National Institues of Health, National Institute of General Medical Sciences (2007)
  • "The Utilization of Detergent Phase Boundary Information for Crystallizing Membrane Proteins" 65th Annual Pittsburgh Diffraction Conference, Pittsburgh Diffraction Society (2007)
  • "The Center for High-Throughput Structural Biology: Show Me the Bottlenecks" Protein Structure Initiative's 6th Bottlenecks Workshop, National Institutes of Health, National Institute of General Medical Sciences (2007)
  • "Oxylipin Biosynthesis in Pathogen-Inducible Oxygenase" Biophysics and Structural Biology Retreat, University of Rochester (2007)
  • "Novel Oxygenations of Arachidonic Acid by Cyclooxygenases" Segal Osteoarthritis Symposium, Northwestern University (2006)
  • "Technology Development at the Center for High-Throughput Structural Biology" Protein Structure Initiative Annual Meeting, National Institues of Health, National Institute of General Medical Sciences (2006)
  • "The Center for High-Throughput Structural Biology" Protein Structure Initiative's 5th Bottlenecks Workshop, National Institutes of Health, National Institute of General Medical Sciences (2006)
  • "Novel Oxygenations of Polyunsaturated Fatty Acids" Upstate New York Structural Biology Day, University of Rochester (2003)
  • "Fatty Acid Interactions with Cyclooxygenases" , Roswell Park Cancer Institute, Department of Molecular and Cellular Biophysics (2001)
  • "Fatty Acid Substrate Interactions with Cyclooxygenases" 10th Buffalo-Hamilton-Toronto Crystallography Meeting (2001)
  • "Fatty Acid Substrate Interactions with Cyclooxygenases" 4th International Workshop on COX-2 (2001)
See all (25 more)
Service Activities:
  • JSMBS Research Strategic Plan Steering Committee; Member (2017–present)
  • BioXFEL Faculty Search Committee; Committee Member (2016–present)
  • PPBS Admissions Commitee; Committee Member (2015–2016)
  • PPBS Steering Committee; Department of Structural Biology representative; Committee Member (2013–present)
  • IGPBS Admissions Assessment Committee; Department of Structural Biology representative; Committee Member (2008)
  • IGPBS Steering Committee; Department of Structural Biology representative; Committee Member (2001–2009)
  • IGPBS Admissions Committee; Department of Structural Biology representative; Committee Member (2001–2003)

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Contact Information

Department of Structural Biology
Hauptman-Woodward Institute
700 Ellicott Street
Buffalo, NY 14203
Phone: 716-898-8624
Fax: 716-898-8660
Email: mgm22@buffalo.edu


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