Satpal Singh PhD
Satpal Singh
PhD
Professor
Department of Pharmacology and Toxicology
Jacobs School of Medicine & Biomedical Sciences
Specialty/Research Focus
Behavioral pharmacology; Cardiac pharmacology; Ion channel kinetics and structure; Membrane Transport (Ion Transport); Molecular Basis of Disease; Neurobiology; Neuropharmacology; Signal Transduction; Transgenic organisms
Professional Summary:
With over 400 genes coding for them in humans, ion channels play a significant role in most physiological functions. Drug-induced channel dysfunction often leads to a variety of disorders and results in significant incidence of serious injury and death. We investigate molecular mechanisms underlying neurodegenerative disorders and cardiac arrhythmias induced by ion channel dysfunction arising from genetic factors and/or drug interactions. The tools used for these investigations include genetic, electrophysiologic, pharmacologic, molecular and cell culturing methods. Preparations used for experiments include Drosophila as a genetic model system, and human cell lines expressing human ion channels that play an important role in critical-to-life functions including cardiac rhythm, respiration and the central nervous system.
Education and Training:
- PhD, Molecular Biology, Tata Institute of Fundamental Research, Bombay, India (1980)
- MS, Physics, Punjab University, Chandigarh, India, Honors (1973)
- BS, Physics, Punjab University, Chandigarh, India, Honors (1972)
Employment:
- Professor, Pharmacology and Toxicology, State University of New York at Buffalo (2016-present)
- Associate Professor, Pharmacology & Toxicology, State University of New York at Buffalo (1997–2016)
- Assistant Professor, State University of New York at Buffalo (1989–1997)
- Visiting Associate Professor, Department of Biology, University of Iowa (1989)
- Assistant Professor, Department of Biology, Guru Nanak Dev (G.N.D.) University (1982–1985)
Research Expertise:
- Channelopathies: With over 400 genes coding for them in humans, ion channels play a significant role in most physiological functions. Drug-induced channel dysfunction often leads to a variety of disorders and results in significant incidence of serious injury and death. We investigate molecular mechanisms underlying neurodegenerative disorders and cardiac arrhythmias induced by ion channel dysfunction arising from genetic factors and/or drug interactions. The tools used for these investigations include genetic, electrophysiologic, pharmacologic, molecular and cell culturing methods. Preparations used for in experiments include Drosophila as a genetic model system, and human cell lines expressing human ion channels that play an important role in critical-to-life functions including cardiac rhythm, respiration and the central nervous system.
UB 2020 Strategic Strengths:
- Molecular Recognition in Biological Systems and Bioinformatics
- Health and Wellness Across the Lifespan
Grants and Sponsored Research:
- September 2011–August 2016
Targeted MSP: The University at Buffalo/Buffalo Public Schools (UB/BPS) Interdisciplinary Science and Engineering Partnership
National Science Foundation
Role: Co-Investigator
$9,831,207 - July 2010–June 2013
Subcontract for Science support for NY State Ed Math Science Partnership - Buffalo Public Schools
New York State Department of Education
Role: Co-Investigator
$254,976 - August 2008–September 2011
A Genetic Model of Leigh Syndrome Suppression in Drosophila
NIH (NINDS)
Role: Principal Investigator
$158,500 - January 2006–December 2009
Interdisciplinary Science Research-based Curricular Innovations for Middle and High School Science and Teacher Professional Development
John R. Oishei Foundation
Role: Co-Investigator
$480,000
Journal Articles:
- Frolov RV, Ignatova II, Singh S. Inhibition of HERG potassium channels by celecoxib and its mechanism. PLoS One. 2011; 6(10).
- Frolov RV, Ignatova II, Singh S. Mechanism of K(v)2.1 channel inhibition by a selective COX-2 inhibitor SC-791-modification of gating. Brain Res. 2010; 1359.
- Frolov RV, Bondarenko VE, Singh S. Mechanisms of Kv2.1 channel inhibition by celecoxib--modification of gating and channel block. Br J Pharmacol. 2010; 159(2).
- Frolov RV, Slaughter MM, Singh S. Effects of celecoxib on ionic currents and spontaneous firing in rat retinal neurons. Neuroscience. 2008; 154(4).
- Frolov RV, Berim IG, Singh S. Inhibition of delayed rectifier potassium channels and induction of arrhythmia: a novel effect of celecoxib and the mechanism underlying it. J Biol Chem. 2008; 283(3).
- W. Liu, R. Gnanasambandam, J. Benjamin, G. Kaur, P. B. Getman, A. J. Siegel, R. D. Shortridge, and S. Singh. Mutations in Cytochrome c Oxidase Subunit VIa Cause Neurodegeneration and Motor Dysfunction in Drosophila. Genetics. 2007; 176(2).
- Bhattacharya A, Lakhman SS, Singh S, Lakhman S. Modulation of L-type calcium channels in Drosophila via a pituitary adenylyl cyclase-activating polypeptide (PACAP)-mediated pathway. J Biol Chem. 2004; 279(36).
- Bhattacharya, A., Lakhman, S. S. and Singh, S.. Modulation of L-type Ca2+ Channels in Drosophila via a Pituitary Adenylyl Cyclase-activating Polypeptide (PACAP)-mediated pathway.. J. Biol. Chem.. 2004; 279.
- Peri R, Triggle DJ, Singh S. Regulation of L-type calcium channels in pituitary GH(4)C(1) cells by depolarization.. J Biol Chem. 2001; 276(34).
- Peri R, Padmanabhan S, Rutledge A, Singh S, Triggle DJ. Permanently charged chiral 1,4-dihydropyridines: molecular probes of L-type calcium channels. Synthesis and pharmacological characterization of methyl(omega-trimethylalkylammonium) 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridinedicarboxylate iodi. J Med Chem. 2000; 43(15).
- Chopra, M., Gu, G.-G. and Singh, S.. Mutations Affecting the Delayed Rectifier Potassium Current in Drosophila.. J. Neurogenetics. 2000; 14.
- Hegde P, Gu GG, Chen D, Free SJ, Singh S. Mutational analysis of the Shab-encoded delayed rectifier K(+) channels in Drosophila. J Biol Chem. 1999; 274(31).
- Bhattacharya A, Gu GG, Singh S. Modulation of dihydropyridine-sensitive calcium channels in Drosophila by a cAMP-mediated pathway. J Neurobiol. 1999; 39(4).
- Singh S, Wu CF. Ionic currents in larval muscles of Drosophila. Int Rev Neurobiol. 1999; 43.
- Hegde, P., Gu, G-G., Chen, D., Free, S. J. and Singh, S.. Mutational Analysis of the Shab-encoded Delayed Rectifier K+ channels in Drosophila.. J. Biol. Chem.. 1999; 274.
- Bhattacharya, A., Gu., G.-G. and Singh S.. Modulation of the Dihydropyridine-Sensitive Calcium Channels in Drosophila by a cAMP-Mediated Pathway.. J. Neurobiol.. 1999; 39.
- Singh, A., and Singh, S.. Unmasking of a Novel Potassium Current in Drosophila by a Mutation and Drugs.. J. Neurosci.. 1999; 19.
- Kraliz, D., Bhattacharya, A. and Singh, S.. Blockade of the Delayed Rectifier Potassium Current in Drosophila by Quinidine and Related Compounds.. J. Neurogenet.. 1998; 12.
- Gu GG, Singh S. Modulation of the dihydropyridine-sensitive calcium channels in Drosophila by a phospholipase C-mediated pathway. J Neurobiol. 1997; 33(3).
- Kraliz, D. and Singh, S.. Selective blockade of the delayed rectifier potassium current by tacrine in Drosophila.. J. Neurobiology. 1997; 32.
- Gu GG, Singh S. Pharmacological analysis of heartbeat in Drosophila. J Neurobiol. 1995; 28(3).
- Gielow ML, Gu GG, Singh S. Resolution and pharmacological analysis of the voltage-dependent calcium channels of Drosophila larval muscles. J Neurosci. 1995; 15(9).
- Chopra M, Singh S. Developmental temperature selectively regulates a voltage-activated potassium current in Drosophila. J Neurobiol. 1994; 25(2).
- Singh, S.. Quantification of countercurrent distribution: from molecular partition to animal behavior. Biochem. Biophys. Res. Comm.. 1993; 196.
- Singh S, Wu CF. Properties of potassium currents and their role in membrane excitability in Drosophila larval muscle fibers. J Exp Biol. 1990; 152.
- Komatsu A, Singh S, Rathe P, Wu CF. Mutational and gene dosage analysis of calcium-activated potassium channels in Drosophila: correlation of micro- and macroscopic currents. Neuron. 1990; 4(2).
- Wu CF, Tsai MC, Chen ML, Zhong Y, Singh S, Lee CY. Actions of dendrotoxin on K+ channels and neuromuscular transmission in Drosophila melanogaster, and its effects in synergy with K+ channel-specific drugs and mutations. J Exp Biol. 1989; 147.
- Singh, A. and Wu, C.-F.. Complete separation of four potassium currents in Drosophila. Neuron. 1989; 2.
- Schlegel, P., Jen, P.H.-S. and Singh, S.. Auditory spatial sensitivity of inferior collicular neurons of echolocating bats. Brain Res.. 1988; 456.
- Singh S Bhandari B Chopra MJS Guha D. Isolation of autosomal mutations in Drosophila melanogaster without setting up lines.. Molec Gen Genet. 1987; 208.
- Neuweiler, G., Singh, S. and Sripathi, K.. Audiograms of a South Indian bat community. J. Comp. Physiol.. 1984; 154.
- Schlegel, P. and Singh, S.. Unmasking in neurons of the inferior colliculus of Eptesicus fuscus with binaural stimulation. Hearing Res.. 1983; 10.
- Singh S Siddiqi O. torpid , a new sex linked temperature paralytic mutation in Drosophila melanogaster. Molec. Gen. Genet.. 1981; 181.
- Peri, R., Padmanabhan, S., Rutledge, A., Singh, A. and Triggle, D.J.. Permanently charged chiral 1,4-dihydropyridines: molecular probes of L-type calcium channels. Synthesis and Pharmacological characterization of methyl (w-trimethylalkylammonium) 1,4-dihydro-2,6-dimethyl-4-(3nitrophenyl)-3,5-pyridinedicarboxylate iodide,. J. Med. Chem.. ; 43.
Abstracts:
- Chopra, M., Gu, G.-G. and Singh, S. Mutations Affecting the Delayed Rectifier Potassium Current in Drosophila. J. Neurogenetics. ; 14.
- Hedge, P., Gu, G-G., Chen, D., Free, S.J. and Singh, S.. Mutational Analysis of the Shab-encoded Delayed Rectifier K+ channels in Drosophila. J. Biol. Chem.. ; 274.
- Singh, A. and Singh, S.. Unmasking of a Novel Potassium Current in Drosophila by a Mutation and Drugs. J. Neurosci.. ; 19.
- Bhattacharya, A., Gu., G.-G. and Singh, S.. Modulation of the Dihydropyridine-Sensitive Calcium Channels in Drosophila by a cAMP-Mediated Pathway. J. Neurobiol.. ; 39.
- Kraliz, D., Bhattacharya, A. and Singh, S.. Blockade of the Delayed Rectifier Potassium Current in Drosophila by Quinidine and Related Compounds. J. Neurogenet.. ; 12.
- Gu G.-G. and Singh, S.. Modulation of the dihydropyridine-sensitive Ca2+ channels in Drosophila by a phospholipase C-mediated pathway. J. Neurobiology. ; 33.
- Kraliz, D. and Singh, S.. Selective Blockade of the delayed rectifier potassium current by tacrine in Drosophila. J. Neurobiology. ; 32.
- Gielow, M.L., Gu, G.-G. and Singh, S.S.. Resolution and pharmacological analysis of the voltage-dependent calcium channels of Drosophila larval muscles. J. Neuroscience. ; 15.
- Satpal Singh. Pharmacological analysis of heartbeat in Drosophila. j. Neurobiology. ; 28.
- Chopra, M. and Singh, S.. Developmental temperature selectively regulates a voltage-activated potassium current in Drosophila. J. Neurobiol.. ; 25.
- Komatsu, A., K., Singh, S., Rathe, P. and Wu, C.-F.. Mutational and gene-dosage analysis of calcium-activated potassium channels in Drosophila: Correlation of micro- and macroscopic currents. Neuron. ; 4.
- Singh, S. and Wu, C.-F.. Properties of potassium currents and their role in membrane excitability in Drosophila larval muscle fibers. J. Exp. Biol.. ; 152.
- Wu, C.-F., Tsai, M.-C., Chen, M.-L., Zhong, Y., Singh, S. and Lee, C.Y.. Actions of dendrotoxin on K+ channels and neuromuscular transmission in Drosophila melanogaster and its effects in synergy with K+ channel-specific drugs and mutations. J. Exp. Biol.. ; 147.
- Singh, S., Bhandari, P., Chopra, M.J.S. and Guha, D.. Isolation of autosomal mutations in Drosophila melanogaster without setting up lines. Molec. Gen. Genet.. ; 208.
- Singh, S.. A mutagenesis scheme for obtaining autosomal mutations in Drosophila. Ind. J. Exp. Biol.. ; 21.
- Singh, S. and Siddiqi, O.. Torpid, a new sex linked temperature paralytic mutation in Drosophila melanogaster. Molec. Gen. Genet.. ; 181.