Yongho Bae PhD

Yongho Bae

Yongho Bae
PhD

Assistant Professor

Department of Pathology and Anatomical Sciences

Jacobs School of Medicine & Biomedical Sciences


Specialty/Research Focus

Apoptosis and cell death; Cell Cycle; Cytoskeleton and cell motility; Gene Expression; Genomics and proteomics; Molecular and Cellular Biology; Molecular Basis of Disease; Signal Transduction

Contact Information
955 Main Street, Room 4260
Pathology and Anatomical Sciences
Buffalo, NY 14203-1121
Phone: 716-829-3523
Fax: 716-829-2911
yonghoba@buffalo.edu



Professional Summary:

I am a cell biologist and bioengineer, and my primary research focuses on the rapidly growing area of cell/tissue mechanics and mechanotransduction: the role that mechanical forces play in regulating cellular function from healthy to diseased phenotypes.

(1) Cardiovascular Biology, Mechanics and Disease:
Cardiovascular disease (CVD) is the main cause of death globally. Arterial stiffness is associated with many CVD. The molecular mechanisms governing arterial stiffening and the phenotypic changes in vascular smooth muscle cells (VSMCs) associated with the stiffening process are key areas in cardiovascular biology, mechanics and disease. Evidence suggests that arterial stiffening can drive aberrant migration and proliferation of VSMCs within the vessel wall. Yet, the underlying mechanisms regulating vascular stiffening and the molecular changes within VSMCs associated with the stiffening process remain unclear. While medications reduce hypertension, none specifically target pathways directly related to arterial stiffness.

The overall goal of work in my lab is to address this gap in our understanding by investigating how changes in arterial stiffness affect VSMC function and fundamentally contribute to the progression of CVD. This study also addresses an important concept in vascular tissue remodeling (the interaction between extracellular matrix stiffness and VSMC behavior). Methodologically, my lab use a novel approach to dissect the molecular mechanism in VSMCs: My lab combines methods for manipulating and measuring tissue and cell stiffness using atomic force microscopy and traction force microscopy for simultaneously modulating substrate stiffness and measuring contraction force by culturing cells on a compliant substrate that mimics in vivo mechanical environments of the VSMCs.

(2) Smooth Muscle Cell (and Cancer Cell) Heterogeneity:
Highly heterogeneous responses of VSMCs to arterial stiffness or CVD make it difficult to dissect underlying molecular mechanisms. To overcome this, my lab integrates Mechanobiology, Vascular Cell Biology, and Machine Learning to manipulate stiffness and assess responses with unique precision. Machine learning is used to deconvolve inter- and intra-cellular heterogeneity and identify specific subcellular traits that correlate with stiffness and VSMC behavior.

My lab also applies Machine Learning approaches to identify specific breast cancer cell behaviors that respond to different stiffness conditions.

(3) Optogenetics and Biophotonics in Stem Cell Biology:
Major breakthroughs in the field of genomics, embryonic stem cell biology, optogenetics and biophotonics are enabling the control and monitoring of biological processes through light. Additional research in my laboratory focuses on developing a nanophotonic platform able to activate/inactivate gene expression and, thus, control stem cell differentiation in neuronal cells, by means of light-controlled protein-protein interactions. More specifically, the light-controlled molecular toggle-switch based on Plant Phytochrome B and transcription factor Pif6 will be utilized to control the nuclear fibroblast growth factor receptor-1, which is a master regulator of stem cell differentiation.

Open Positions: The Bae lab is currently accepting graduate students through the Pathology Masters program (or other programs) as well as motivated undergraduates.

For Graduate Students: I am looking for one or two graduate (MS) students who understand my research interests, have read my previous publications, and have their own (crazy!!!) ideas as to where my research efforts should be directed. All graduate students are required to complete and submit internationally recognized Journal article before graduation from my lab. A Masters thesis should generate at least one first author publication.

For Undergraduate Students: I encourage all UB undergraduates (with GPA 3.0 or higher) to get "hands on" experimental training in the sciences. An undergraduate research project tends to be part of a larger whole, but I make sure to include credit for students work in presentations and publications.

Education and Training:

  • PhD, Bioengineering, University of Pittsburgh (2010)
  • MS, Chemical and Biochemical Engineering, Rutgers University, The State University of New Jersey (2005)
  • BS, Biotechnology, AJou University (1998)

Employment:

  • Assistant Professor, Pathology and Anatomical Sciences, University at Buffalo (2017-present)
  • Research Associate, Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, Perelman School of Medicine (2015–2016)
  • Post Doctoral Fellow, Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, Perelman School of Medicine (2010–2015)
  • American Heart Association Postdoctoral Fellow, Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, Perelman School of Medicine (2013–2014)

Awards and Honors:

  • Eugene Mindell & Harold Brody Clinical Translational Research Award (2017)
  • Cell & Molecular Bioengineering Meeting: Fellow Travel Award (2015)
  • American Heart Association Postdoctoral Fellowship (2013)

Research Expertise:

  • Atomic Force Microscopy
  • Cell and Cancer Biology
  • Cell Mechanics and Mechanobiology
  • Optogenetics and Optogenomics

Grants and Sponsored Research:

  • August 2017–July 2020
    Networked Nanophotonic Devices for Stem Cell Regulation: From Optogenetics to Optogenomics
    National Science Foundation
    Role: Co-Principal Investigator
    $599,067
  • January 2013–December 2014
    American Heart Association Postdoctoral Fellowship
    American Heart Association
    Role: Principal Investigator
    $93,000

Journal Articles:

See all (4 more)

Books and Book Chapters:


Professional Memberships:

  • Cellular and Molecular Bioengineering (2014–present)
  • American Heart Association (2012–present)
  • Biomedical Engineering Society (2008–present)
  • American Society for Cell Biology (2007–present)

Presentations:

  • "Deconvolution of Subcellular Protrusion Heterogeneity by Machine Learning-Based Live Cell Analysis" Quantitative Biology Meeting (2018)
  • "Nanophotonics and Optogenetics- A Novel Combination towards Precise Stem Cell Regulation" American Society for Cell Biology/EMBO (2017)
  • "Apolipoprotein E interrupts a positive feedback loop that links collagen-I synthesis to intracellular stiffness" Biomedical Engineering Society, Cellular and Molecular Bioengineering (2015)
  • "A FAK-Cas-Rac-Lamellipodin Signaling Module Transduces Extracellular Matrix Stiffness into Mechanosensitive Cell Cycling" Gordon Research Conference, Signal Transduction by Engineered Extracellular Matrices (2014)
  • "Matrix metalloproteinase-12 controls arterial stiffness in vascular remodeling" American Heart Association, Scientific Session (2012)
  • "The FAK/Src/p130Cas pathway controls stiffness-dependent Rac activation and cell cycling" Gordon Research Conference, Signaling by Adhesion Receptors (2012)
  • "A Novel Actin-independent Anti-migratory Function of Profilin1 in Breast Cancer Cells" American Society for Cell Biology (2010)
  • "Membrane phosphoinositide availability, regulated by profilin-1, determines breast cancer cell motility secondary to binding of lamellipodin" American Society for Cell Biology (2009)
  • "Turning Profilin-1 to an anti-migratory molecule in breast cancer cells - molecular dissection" Biomedical Engineering Society (2009)
  • "Loss of Profilin-1 expression leads to hypermotile phenotype of breast cancer cell via lamellipodial targeting of Ena/VASP" American Society for Cell Biology (2008)
  • "Profilin is a negative regulator of mammary carcinoma aggressiveness" American Association for Cancer Research (2007)
  • "Evaluation of the effect of the chemistry of biodegradable polycarbonate polymers containing iodine and PEG on cellular response of vascular cell lines" American Institute of Chemical Engineers (2005)
See all (2 more)

Service Activities:

  • Jacobs School of Medicine & Biomedical Sciences Admissions Committee; Member (2018)
  • Editorial Board; Journal of Microbiology and Pathology; Editorial Board Member (2017–present)
  • Editorial Review Board; Journal of Biomedical Engineering and Informatics; Editorial Board Member (2015–present)
  • Reviewer for Journal Articles; Cancer Research, PLoS One, Integrative Biology, Current Biotechnology, Journal of Tissue Engineering, Journal of the Mechanical Behavior of Biomedical Materials, Journal of Vascular Research and Cellulose; Peer Reviewer

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Contact Information

955 Main Street, Room 4260
Pathology and Anatomical Sciences
Buffalo, NY 14203-1121
Phone: 716-829-3523
Fax: 716-829-2911
yonghoba@buffalo.edu