Confocal Microscopy/Cellular Imaging

Services

This facility contains a Zeiss LSM-510 Meta NLO laser scanning confocal microscope attached to a Zeiss-inverted microscope. It is capable of simultaneous imaging of multiple-emission wavelengths, transmitted-light imaging and multiphoton capability through a Coherent Chameleon Ultra II laser.

A total of seven laser excitation lines (one tunable via the Chameleon laser), two single-wavelength emission channels and the multispectral “meta” detector allow a large number of dye combinations to be imaged in an individual specimen.

A microscope incubator and temperature controller is available for live-cell imaging.

Zeiss Axio Observer and Axioimager wide-field fluorescent microscopes are available for bright field, DIC and phase contrast imaging, complete with cooled-CCD and color cameras.

These are also equipped with Zeiss’s Apotome structured-illumination system and deconvolution software for forming high-resolution optical slices of objects not well suited for confocal imaging. Image analysis and volume rendering software take the acquired datasets and perform 3D reconstruction of cellular structures.

The Leica AS/LMD laser capture/laser micro-dissection system is available for isolation of single cells or small pieces of tissue from histological or live specimens.

Equipment

scanning of isotope-labeled gels with the Bio-Rad Personal FX phosphoimager imaging of ethidium bromide stained DNA gels with the Bio-Rad Gel Doc and a Bio-Rad GS-700 flatbed scanning densitometer for film and gel scanning and flatbed scanning densitometry
electronic chemiluminescent imaging of Western blots with the Fuji LAS-1000
two Bio-Rad real time PCR thermocyclers (multiplex iQ and MyiQ)
nanodrop ND-1000 spectrophotometer
Bio-Tek Synergy Absorbance, Fluorescence, and Luminescence plate reader
Becton Dickinson FACS array bioanalyzer multiplexed bead and cellular assays
Zeiss Elispot automated cytokine analysis imaging system