This facility offers high-throughput, next-generation sequencing using the Roche/454 Genome Sequencer and Illumina Genome Analyzer platforms. We also provide gene expression analysis services on Affymetrix platforms, including 3’-expression, miRNA, exon and gene arrays.

Our Affymetrix Gene Chip System comprises a GeneChip Scanner 3000, two Fluidics 450 units and a GeneChip Hybridization Oven 640.

This system provides expression analysis services on all Affymetrix Gene Chips, including 3’-expression, miRNA, exon and gene arrays.

It performs sample quality analysis using the Agilent 2100 Bioanalyzer, target labeling, fragmentation, hybridization, staining and scanning.

Lab personnel conduct initial data analysis. You can arrange dditional data analysis services with bioinformatics specialists at UB or Roswell Park Cancer Institute.

Our Roche/454 Genome Sequencer FLX Titanium platform generates about 1,000,000 reads with an average length of 300-500bp in a typical run. It is an excellent platform for de novo sequencing and for projects that require longer read lengths.

The GS FLX Titanium accommodates shotgun, paired-end and amplicon sequencing runs. The lab performs genomic DNA preparation, GS FLX library preparation, titrations, emPCR and sequencing runs.

Our illumina HiSeq 2000 platform supports a variety of different experiments, including whole-genome and candidate-region resequencing, transcriptome analysis, small RNA discovery, methylation profiling and protein-nucleic acid interaction analysis at a genome-wide scale. It is an excellent platform for projects that need broad coverage and can accommodate short read lengths.

• Affymetrix Gene Chip System comprising a GeneChip Scanner 3000, two Fluidics 450 units and a GeneChip Hybridization Oven 640
• illumina HiSeq 2000
• Roche/454 Genome Sequencer FLX Titanium

UB New York State Center of Excellence in Bioinformatics and Life Sciences
Buffalo Niagara Medical Campus
701 Ellicott Street
Buffalo, N.Y.