Richard M. Gronostajski PhD
Richard M. Gronostajski
PhD
Professor of Biochemistry; Director of the Genetics, Genomics & Bioinformatics Graduate Program; Director, Western New York Stem Cell Culture and Analysis Center (WNYSTEM)
Jacobs School of Medicine & Biomedical Sciences
Specialty/Research Focus
Bioinformatics; Cell growth, differentiation and development; Gene Expression; Genomics and proteomics; Molecular and Cellular Biology; Molecular Basis of Disease; Molecular genetics; Neurobiology; Stem Cells; Transgenic organisms
Professional Summary:
My research goal is to gain a better understanding of how proteins that interact with DNA regulate RNA transcription, DNA replication and metazoan development. I mentor undergraduate and graduate students in my lab; we focus on the structure and function of the Nuclear Factor I (NFI) family of site-specific DNA binding proteins, and we are investigating their roles in development. Our work has been made possible by our development of loss-of-function mutations of the NFI genes in the mouse and C. elegans.
We are addressing four major questions in my laboratory and in collaboration with a number of talented collaborators: What is the structure of the NFI DNA-binding domain? How does NFI recognize and interact with DNA? Does NFI change the structure of DNA when it binds? What proteins interact with NFI to stimulate RNA transcription and/or DNA replication?
These research questions are explored in my lab through two major projects focused on the role of NFIB in lung development and the role of NFIX in brain development.
When NFIB is deleted from the germline of mice the animals die at birth because their lungs fail to mature normally. This provides a good model for the problems that occur with premature infants, whose lungs also fail to mature normally. We are using this model to determine how NFIB promotes lung maturation with the goal of being able to stimulate this process in premature infants.
In our NFIX knockout animals, the brains of the animals are actually larger than normal and contain large numbers of cells in an area known to be the site of postnatal neurogenesis. We have evidence that NFIX may regulate the proliferation and differentiation of neural stem cells, which produce new neurons throughout adult life. Our aim is to understand the specific target genes that NFIX regulates in the adult brain to control this process of neurogenesis.
Education and Training:
- PhD, Physiology, Harvard University (1982)
- MS, Biochemistry, Virginia Polytechnic Institute and State University (1977)
- BS, Biochemistry, Virginia Polytechnic Institute and State University, With Honors (1975)
Employment:
- Director: Genetics, Genomics & Bioinformatics, Genetics, Genomics and Bioinformatics, University at Buffalo (2013-present)
- Professor, Biochemistry, 140 Farber Hall, University at Buffalo (2001-present)
- Associate Professor, Biochemistry, Case Western Reserve University, School of Medicine (1992–2001)
- Assistant Professor, Medical Biophysics, Ontario Cancer Institute, University of Toronto, Canada (1985–1992)
Research Expertise:
- : Studies on NFI can be divided into two major themes: (1) biochemical analysis of NFI protein structure and function and (2) molecular genetic studies on NFI‘s role in cell growth, differentiation and development.
- : (1) The DNA-binding domain of NFI differs from those found in other well characterized DNA-binding proteins. Four major questions being addressed in the laboratory are: What is the structure of the NFI DNA-binding domain? How does NFI recognize and interact with DNA? Does NFI change the structure of DNA when it binds? What proteins interact with NFI to stimulate RNA transcription and/or DNA replication?
- : We have shown that the NFI-C protein represses the glucocorticoid-dependent expression of the MMTV promoter. This repression can be overcome by overexpression of the co-activator proteins CBP, p300 or SRC-1, suggesting a role of these co-activators in MMTV expression. Surprisingly, NFI-C doesn‘t repress progesterone stimulation of MMTV. We are currently working out the biochemical mechanism for this repression by NFI-C and the roles of co-activators, histone acetylase activity and chromatin remodeling activity in the process.
- : (2) Using the mouse embryonic stem cell (ES cell) system and human leukemic cells, the role of NFI family members during cell differentiation is being studied. We showed previously that the forms of NFI present in ES and leukemic cells change during the differentiation of the cells in vitro. Currently we are asking the questions: By what mechanism does the form of NFI change during differentiation? How does the expression of different NFI family members affect pathways of differentiation? Is the expression of specific NFI family members required for the synthesis of specific classes of proteins in terminally differentiated cell types? What is the phenotype of mutant mice deficient in one or more NFI gene?
- : The NFI-A deficient mouse we generated (Nfia-) has major neurological defects including agenesis of the corpus callosum and hydrocephalus. We‘re now studying the biochemical pathways leading to these developmental defects with the goal of determining how loss of a single transcription factor results in major neuroanatomical changes. We‘re focusing on whether loss of NFI-A causes changes in: 1) cell proliferation or death, 2) cell migration or differentiation, 3) axonal outgrowth, 4) axonal pathfinding, 5) glial cell differentiation and 6) patterns of neuronal or glial cell gene expression.
- : While all vertebrates examined contain 4 highly conserved NFI genes (NFI-A, -B, -C and -X), the nematode Caenorhabditis elegans has only a single NFI gene (nfi-1). Unlike the case in vertebrates, where all 4 NFI genes are expressed in many tissues during both embryogenesis and throughout adult life, the C. elegans nfi-1 gene is expressed primarily during embryogenesis. In collaboration with Yuji Kohara we‘ve identified where nfi-1 mRNA is expressed in C. elegans and are assessing the phenotype of worms deficient in the nfi-1 gene product. By comparing the function of NFI in worms and mice, we are asking how NFI-dependent developmental pathways have been conserved through over 500M years of evolution.
- : The goal of our laboratory is to gain a better understanding of how proteins that interact with DNA regulate RNA transcription and metazoan development. Our focus is on the structure and function of the Nuclear Factor I (NFI) family of site-specific DNA binding proteins. In vertebrates, NFI family members function in both the replication of viral DNA and the transcription of viral and cellular genes. We are currently analyzing the role of the NFI gene family in both vertebrate and C. elegans development.
Research Centers:
- Center of Excellence in Bioinformatics and Life Sciences
UB 2020 Strategic Strengths:
- Molecular Recognition in Biological Systems and Bioinformatics
- Health and Wellness Across the Lifespan
Grants and Sponsored Research:
- September 2016–August 2021
Stem Cells in Regenerative Medicine (SCiRM)
NYSTEM (NYSDOH)
Role: Contributor
$1,750,000 - December 2015–November 2018
Role of Nfi genes in neural stem cell homeostasis
NYSTEM (NYSDOH)
Role: Principal Investigator
$1,073,067 - August 2011–July 2015
WNYSTEM, Western New York Stem Cell Culture and Analysis Center
NYSTEM
Role: Principal Investigator
$3,564,599 - September 2010–August 2013
Role of Nfix in Neural Stem Cells and glioblastoma
NYSTEM
Role: Co-Investigator
$1,061,683 - March 2007–February 2013
Role of NFI genes in mouse lung development
NIH
Role: Principal Investigator
$1,981,250 - –August 2005
Genes & Proteins Needed for NFI-regulated Transcription
NIDDK
Role: Principal Investigator
$175,000 - April 2002–March 2005
Novel Genes expressed during Kidney Development
NIDDK
Role: Principal Investigator
$215,858 - November 2001–March 2004
Novel Genes Expressed during Kidney Development
NIDDK
Role: Principal Investigator
$259,593
Journal Articles:
- Oishi S, Harkins D, Kurniawan ND, Kasherman M, Harris L, Zalucki O, Gronostajski RM, Burne THJ, Piper M. Heterozygosity for Nuclear Factor One X in mice models features of Malan syndrome. EBioMedicine. 2019; 39.
- Zalucki O, Harris L, Harvey TJ, Harkins D, Widagdo J, Oishi S, Matuzelski E, Yong XLH, Schmidt H, Anggono V, Burne THJ, Gronostajski RM, Piper M. NFIX-Mediated Inhibition of Neuroblast Branching Regulates Migration Within the Adult Mouse Ventricular-Subventricular Zone. Cereb Cortex. 2018.
- Zalucki O, Harkins D, Harris L, Burne THJ, Gronostajski RM, Piper M. Analysis of hippocampal-dependent learning and memory behaviour in mice lacking Nfix from adult neural stem cells. BMC Res Notes. 2018; 11(1).
- Ding B, Dobner PR, Mullikin-Kilpatrick D, Wang W, Zhu H, Chow CW, Cave JW, Gronostajski RM, Kilpatrick DL. BDNF activates an NFI-dependent neurodevelopmental timing program by sequestering NFATc4. Mol Biol Cell. 2018; 29(8).
- Vidovic D, Davila RA, Gronostajski RM, Harvey TJ, Piper M. Transcriptional regulation of ependymal cell maturation within the postnatal brain. Neural Dev. 2018; 13(1).
- Harris L, Zalucki O, Clément O, Fraser J, Matuzelski E, Oishi S, Harvey TJ, Burne THJ, Heng JI, Gronostajski RM, Piper M. Neurogenic differentiation by hippocampal neural stem and progenitor cells is biased by NFIX expression. Development. 2018; 145(3).
- Harris L, Zalucki O, Gobius I, McDonald H, Osinki J, Harvey TJ, Essebier A, Vidovic D, Gladwyn-Ng I, Burne TH, Heng JI, Richards LJ, Gronostajski RM, Piper M. Correction: Transcriptional regulation of intermediate progenitor cell generation during hippocampal development (doi: 10.1242/dev.140681). Development. 2018; 145(14).
- Matuzelski E, Bunt J, Harkins D, Lim JWC, Gronostajski RM, Richards LJ, Harris L, Piper M. Transcriptional regulation of Nfix by NFIB drives astrocytic maturation within the developing spinal cord. Dev Biol. 2017; 432(2).
- Becker-Santos DD, Lonergan KM, Gronostajski RM, Lam WL. Nuclear Factor I/B: A Master Regulator of Cell Differentiation with Paradoxical Roles in Cancer. EBioMedicine. 2017; 22.
- Chen KS, Harris L, Lim JWC, Harvey TJ, Piper M, Gronostajski RM, Richards LJ, Bunt J. Differential neuronal and glial expression of nuclear factor I proteins in the cerebral cortex of adult mice. J Comp Neurol. 2017; 525(11).
- Fraser J, Essebier A, Gronostajski RM, Boden M, Wainwright BJ, Harvey TJ, Piper M. Cell-type-specific expression of NFIX in the developing and adult cerebellum. Brain Struct Funct. 2017; 222(5).
- Harris L, Zalucki O, Gobius I, McDonald H, Osinki J, Harvey TJ, Essebier A, Vidovic D, Gladwyn-Ng I, Burne TH, Heng JI, Richards LJ, Gronostajski RM, Piper M. Transcriptional regulation of intermediate progenitor cell generation during hippocampal development. Development. 2016; 143(24).
- Wu N, Jia D, Ibrahim AH, Bachurski CJ, Gronostajski RM, MacPherson D. NFIB overexpression cooperates with Rb/p53 deletion to promote small cell lung cancer. Oncotarget. 2016; 7(36).
- Ding B, Cave JW, Dobner PR, Mullikin-Kilpatrick D, Bartzokis M, Zhu H, Chow CW, Gronostajski RM, Kilpatrick DL. Reciprocal autoregulation by NFI occupancy and ETV1 promotes the developmental expression of dendrite-synapse genes in cerebellar granule neurons. Mol Biol Cell. 2016; 27(9).
- Grabowska MM, Kelly SM, Reese AL, Cates JM, Case TC, Zhang J, DeGraff DJ, Strand DW, Miller NL, Clark PE, Hayward SW, Gronostajski RM, Anderson PD, Matusik RJ. Nfib Regulates Transcriptional Networks That Control the Development of Prostatic Hyperplasia. Endocrinology. 2016; 157(3).
- Heng YH, Zhou B, Harris L, Harvey T, Smith A, Horne E, Martynoga B, Andersen J, Achimastou A, Cato K, Richards LJ, Gronostajski RM, Yeo GS, Guillemot F, Bailey TL, Piper M. NFIX Regulates Proliferation and Migration Within the Murine SVZ Neurogenic Niche. Cereb Cortex. 2015; 25(10).
- Vidovic D, Harris L, Harvey TJ, Evelyn Heng YH, Smith AG, Osinski J, Hughes J, Thomas P, Gronostajski RM, Bailey TL, Piper M. Expansion of the lateral ventricles and ependymal deficits underlie the hydrocephalus evident in mice lacking the transcription factor NFIX. Brain Res. 2015; 1616.
- O'Connor C, Campos J, Osinski JM, Gronostajski RM, Michie AM, Keeshan K. Nfix expression critically modulates early B lymphopoiesis and myelopoiesis. PLoS One. 2015; 10(3).
- Harris L, Genovesi LA, Gronostajski RM, Wainwright BJ, Piper M. Nuclear factor one transcription factors: Divergent functions in developmental versus adult stem cell populations. Dev Dyn. 2015; 244(3).
- Lee DS, Choung HW, Kim HJ, Gronostajski RM, Yang YI, Ryoo HM, Lee ZH, Kim HH, Cho ES, Park JC. NFI-C regulates osteoblast differentiation via control of osterix expression. Stem Cells. 2014; 32(9).
- Grabowska MM, Elliott AD, DeGraff DJ, Anderson PD, Anumanthan G, Yamashita H, Sun Q, Friedman DB, Hachey DL, Yu X, Sheehan JH, Ahn JM, Raj GV, Piston DW, Gronostajski RM, Matusik RJ. NFI transcription factors interact with FOXA1 to regulate prostate-specific gene expression. Mol Endocrinol. 2014; 28(6).
- Robinson GW, Kang K, Yoo KH, Tang Y, Zhu BM, Yamaji D, Colditz V, Jang SJ, Gronostajski RM, Hennighausen L. Coregulation of genetic programs by the transcription factors NFIB and STAT5. Mol Endocrinol. 2014; 28(5).
- Lajoie M, Hsu YC, Gronostajski RM, Bailey TL. An overlapping set of genes is regulated by both NFIB and the glucocorticoid receptor during lung maturation. BMC Genomics. 2014; 15.
- Piper M, Barry G, Harvey TJ, McLeay R, Smith AG, Harris L, Mason S, Stringer BW, Day BW, Wray NR, Gronostajski RM, Bailey TL, Boyd AW, Richards LJ. NFIB-mediated repression of the epigenetic factor Ezh2 regulates cortical development. J Neurosci. 2014; 34(8).
- Heng YH, McLeay RC, Harvey TJ, Smith AG, Barry G, Cato K, Plachez C, Little E, Mason S, Dixon C, Gronostajski RM, Bailey TL, Richards LJ, Piper M. NFIX regulates neural progenitor cell differentiation during hippocampal morphogenesis. Cereb Cortex. 2014; 24(1).
- Dixon C, Harvey TJ, Smith AG, Gronostajski RM, Bailey TL, Piper M. Nuclear factor one X regulates Bobby sox during development of the mouse forebrain. Cell Mol Neurobiol. 2013; 33(7).
- Martynoga B, Mateo JL, Zhou B, Andersen J, Achimastou A, Urbán N, van den Berg D, Georgopoulou D, Hadjur S, Wittbrodt J, Ettwiller L, Piper M, Gronostajski RM, Guillemot F. Epigenomic enhancer annotation reveals a key role for NFIX in neural stem cell quiescence. Genes Dev. 2013; 27(16).
- Harris L, Dixon C, Cato K, Heng YH, Kurniawan ND, Ullmann JF, Janke AL, Gronostajski RM, Richards LJ, Burne TH, Piper M. Heterozygosity for nuclear factor one x affects hippocampal-dependent behaviour in mice. PLoS One. 2013; 8(6).
- Chang CY, Pasolli HA, Giannopoulou EG, Guasch G, Gronostajski RM, Elemento O, Fuchs E. NFIB is a governor of epithelial-melanocyte stem cell behaviour in a shared niche. Nature. 2013; 495(7439).
- Ding B, Wang W, Selvakumar T, Xi HS, Zhu H, Chow CW, Horton JD, Gronostajski RM, Kilpatrick DL. Temporal regulation of nuclear factor one occupancy by calcineurin/NFAT governs a voltage-sensitive developmental switch in late maturing neurons. J Neurosci. 2013; 33(7).
- Plachez C, Cato K, McLeay RC, Heng YH, Bailey TL, Gronostajski RM, Gronostasjki RM, Richards LJ, Puche AC, Piper M. Expression of nuclear factor one A and -B in the olfactory bulb. J Comp Neurol. 2012; 520(14).
- Heng YH, McLeay RC, Harvey TJ, Smith AG, Barry G, Cato K, Plachez C, Little E, Mason S, Dixon C, Gronostajski RM, Bailey TL, Richards LJ, Piper M. NFIX Regulates Neural Progenitor Cell Differentiation During Hippocampal Morphogenesis. Cereb Cortex. 2012.
- Kang P, Lee HK, Glasgow SM, Finley M, Donti T, Gaber ZB, Graham BH, Foster AE, Novitch BG, Gronostajski RM, Deneen B. Sox9 and NFIA coordinate a transcriptional regulatory cascade during the initiation of gliogenesis. Neuron. 2012; 74(1).
- Piper M, Harris L, Barry G, Heng YH, Plachez C, Gronostajski RM, Richards LJ, Gronostajski R. Nuclear factor one X regulates the development of multiple cellular populations in the postnatal cerebellum. J Comp Neurol. 2011; 519(17).
- Subramanian L, Sarkar A, Shetty AS, Muralidharan B, Padmanabhan H, Piper M, Monuki ES, Bach I, Gronostajski RM, Richards LJ, Tole S, Gronostajski R. Transcription factor Lhx2 is necessary and sufficient to suppress astrogliogenesis and promote neurogenesis in the developing hippocampus. Proc Natl Acad Sci U S A. 2011; 108(27).
- Hsu YC, Osinski J, Campbell CE, Litwack ED, Wang D, Liu S, Bachurski CJ, Gronostajski RM, Gronostajski R. Mesenchymal nuclear factor I B regulates cell proliferation and epithelial differentiation during lung maturation. Dev Biol. 2011; 354(2).
- Meng F, Suchyna TM, Lazakovitch E, Gronostajski RM, Sachs F, Gronostajski R. Real Time FRET Based Detection of Mechanical Stress in Cytoskeletal and Extracellular Matrix Proteins. Cell Mol Bioeng. 2011; 4(2).
- Muthusamy N, Chen HC, Rajgolikar G, Butz KG, Frissora FW, Gronostajski RM, Gronostajski R. Recombination activation gene-2-deficient blastocyst complementation analysis reveals an essential role for nuclear factor I-A transcription factor in T-cell activation. Int Immunol. 2011; 23(6).
- Gronostajski RM, Guaneri J, Lee DH, Gallo SM, Gronostajski R. The NFI-Regulome Database: A tool for annotation and analysis of control regions of genes regulated by Nuclear Factor I transcription factors. J Clin Bioinforma. 2011; 1(1).
- Kilpatrick DL, Wang W, Gronostajski R, Litwack ED. Nuclear Factor I and Cerebellar Granule Neuron Development: An Intrinsic-Extrinsic Interplay. Cerebellum. 2010.
- Zheng S, Eacker SM, Hong SJ, Gronostajski RM, Dawson TM, Dawson VL. NMDA-induced neuronal survival is mediated through nuclear factor I-A in mice. J Clin Invest. 2010; 120(7).
- Wang W, Crandall JE, Litwack ED, Gronostajski RM, Kilpatrick DL, Gronostajski R. Targets of the nuclear factor I regulon involved in early and late development of postmitotic cerebellar granule neurons. J Neurosci Res. 2010; 88(2).
- Schneegans T, Borgmeyer U, Hentschke M, Gronostajski RM, Schachner M, Tilling T, Gronostajski R. Nuclear factor I-A represses expression of the cell adhesion molecule L1. BMC Mol Biol. 2009; 10(1).
- Piper M, Moldrich RX, Lindwall C, Little E, Barry G, Mason S, Sunn N, Kurniawan ND, Gronostajski RM, Richards LJ, Gronostajski R. Multiple non-cell-autonomous defects underlie neocortical callosal dysgenesis in Nfib-deficient mice. Neural Dev. 2009; 4(1).
- Plasari G, Calabrese A, Dusserre Y, Gronostajski RM, McNair A, Michalik L, Mermod N, Gronostajski R. Nuclear factor I-C links platelet-derived growth factor and transforming growth factor beta1 signaling to skin wound healing progression. Mol Cell Biol. 2009; 29(22).
- Whittle CM, Lazakovitch E, Gronostajski RM, Lieb JD, Gronostajski R. DNA-binding specificity and in vivo targets of Caenorhabditis elegans nuclear factor I. Proc Natl Acad Sci U S A. 2009; 106(29).
- Lee DS, Park JT, Kim HM, Ko JS, Son HH, Gronostajski RM, Cho MI, Choung PH, Park JC, Gronostajski R. Nuclear factor I-C is essential for odontogenic cell proliferation and odontoblast differentiation during tooth root development. J Biol Chem. 2009; 284(25).
- Kumbasar A, Plachez C, Gronostajski RM, Richards LJ, Litwack ED, Gronostajski R. Absence of the transcription factor Nfib delays the formation of the basilar pontine and other mossy fiber nuclei. J Comp Neurol. 2009; 513(1).
- Mason S, Piper M, Gronostajski RM, Richards LJ, Gronostajski R. Nuclear factor one transcription factors in CNS development. Mol Neurobiol. 2009; 39(1).
- Barry G, Piper M, Lindwall C, Moldrich R, Mason S, Little E, Sarkar A, Tole S, Gronostajski RM, Richards LJ, Gronostajski R. Specific glial populations regulate hippocampal morphogenesis. J Neurosci. 2008; 28(47).
- Lazakovitch E, Kalb JM, Gronostajski RM, Gronostajski R. Lifespan extension and increased pumping rate accompany pharyngeal muscle-specific expression of nfi-1 in C. elegans. Dev Dyn. 2008; 237(8).
- Plachez C, Lindwall C, Sunn N, Piper M, Moldrich RX, Campbell CE, Osinski JM, Gronostajski RM, Richards LJ, Gronostajski R. Nuclear factor I gene expression in the developing forebrain. J Comp Neurol. 2008; 508(3).
- Lu W, Quintero-Rivera F, Fan Y, Alkuraya FS, Donovan DJ, Xi Q, Turbe-Doan A, Li QG, Campbell CG, Shanske AL, Sherr EH, Ahmad A, Peters R, Rilliet B, Parvex P, Bassuk AG, Harris DJ, Ferguson H, Kelly C, Walsh CA, Gronostajski RM, Devriendt K, Higgins A, Ligon AH, Quade BJ, Morton CC, Gusella JF, Maas RL, Gronostajski R. NFIA haploinsufficiency is associated with a CNS malformation syndrome and urinary tract defects. PLoS Genet. 2007; 3(5).
- Wong YW, Schulze C, Streichert T, Gronostajski RM, Schachner M, Tilling T, Gronostajski R. Gene expression analysis of nuclear factor I-A deficient mice indicates delayed brain maturation. Genome Biol. 2007; 8(5).
- Deneen B, Ho R, Lukaszewicz A, Hochstim CJ, Gronostajski RM, Anderson DJ, Gronostajski R. The transcription factor NFIA controls the onset of gliogenesis in the developing spinal cord. Neuron. 2006; 52(6).
- Butz NV, Gronostajski RM, Campbell CE, Gronostajski R. T-box proteins differentially activate the expression of the endogenous interferon gamma gene versus transfected reporter genes in non-immune cells. Gene. 2006; 377.
- Lazakovitch E, Kalb JM, Matsumoto R, Hirono K, Kohara Y, Gronostajski RM, Gronostajski R. nfi-I affects behavior and life-span in C. elegans but is not essential for DNA replication or survival. BMC Dev Biol. 2005; 5.
- Steele-Perkins G, Plachez C, Butz KG, Yang G, Bachurski CJ, Kinsman SL, Litwack ED, Richards LJ, Gronostajski RM, Gronostajski R. The transcription factor gene Nfib is essential for both lung maturation and brain development. Mol Cell Biol. 2005; 25(2).
- Wang W, Stock RE, Gronostajski RM, Wong YW, Schachner M, Kilpatrick DL, Gronostajski R. A role for nuclear factor I in the intrinsic control of cerebellar granule neuron gene expression. J Biol Chem. 2004; 279(51).
- Ling G, Hauer CR, Gronostajski RM, Pentecost BT, Ding X. Transcriptional Regulation of Rat CYP2A3 by Nuclear Factor 1: IDENTIFICATION OF A NOVEL NFI-A ISOFORM, AND EVIDENCE FOR TISSUE-SELECTIVE INTERACTION OF NFI WITH THE CYP2A3 PROMOTER IN VIVO.. J Biol Chem. 2004; 279(27).
- Bachurski CJ, Yang GH, Currier TA, Gronostajski RM, Hong D. Nuclear factor I/thyroid transcription factor 1 interactions modulate surfactant protein C transcription.. Mol Cell Biol. 2003; 23(24).
- Messam CA, Hou J, Gronostajski RM, Major EO. Lineage pathway of human brain progenitor cells identified by JC virus susceptibility.. Ann Neurol. 2003; 53(5).
- Murtagh J, Martin F, Gronostajski RM. The Nuclear Factor I (NFI) gene family in mammary gland development and function.. J Mammary Gland Biol Neoplasia. 2003; 8(2).
- Kido K, Bannert H, Gronostajski RM, Flügel RM. Bel1-mediated transactivation of the spumaretroviral internal promoter is repressed by nuclear factor I.. J Biol Chem. 2003; 278(14).
- Pan L, Glenn ST, Jones CA, Gronostajski RM, Gross KW. Regulation of renin enhancer activity by nuclear factor I and Sp1/Sp3.. Biochim Biophys Acta. 2003; 1625(3).
- Steele-Perkins G, Butz KG, Lyons GE, Zeichner-David M, Kim HJ, Cho MI, Gronostajski RM. Essential role for NFI-C/CTF transcription-replication factor in tooth root development.. Mol Cell Biol. 2003; 23(3).
- Shu T, Butz KG, Plachez C, Gronostajski RM, Richards LJ. Abnormal development of forebrain midline glia and commissural projections in Nfia knock-out mice.. J Neurosci. 2003; 23(1).
- Mukhopadhyay SS, Wyszomierski SL, Gronostajski RM, Rosen JM. Differential interactions of specific nuclear factor I isoforms with the glucocorticoid receptor and STAT5 in the cooperative regulation of WAP gene transcription.. Mol Cell Biol. 2001; 21(20).
- Majumder S, Ghoshal K, Gronostajski RM, Jacob ST. Downregulation of constitutive and heavy metal-induced metallothionein-I expression by nuclear factor I.. Gene Expr. 2001; 9(4-5).
- Majumder S, Ghoshal K, Gronostajski RM, Jacob ST. Downregulation of constitutive and heavy metal-induced metallothionein-I expression by nuclear factor I.. Gene Expr. 2001; 9(4-5).
- Sinha S, Abraham S, Gronostajski RM, Campbell CE. Differential DNA binding and transcription modulation by three T-box proteins, T, TBX1 and TBX2.. Gene. 2000; 258(1-2).
- Gronostajski RM. Roles of the NFI/CTF gene family in transcription and development.. Gene. 2000; 249(1-2).
- Behrens M, Venkatraman G, Gronostajski RM, Reed RR, Margolis FL. NFI in the development of the olfactory neuroepithelium and the regulation of olfactory marker protein gene expression.. Eur J Neurosci. 2000; 12(4).
- das Neves L, Duchala CS, Tolentino-Silva F, Haxhiu MA, Colmenares C, Macklin WB, Campbell CE, Butz KG, Gronostajski RM, Godinho F. Disruption of the murine nuclear factor I-A gene (Nfia) results in perinatal lethality, hydrocephalus, and agenesis of the corpus callosum.. Proc Natl Acad Sci U S A. 1999; 96(21).
- Baumeister H, Gronostajski RM, Lyons GE, Margolis FL. Identification of NFI-binding sites and cloning of NFI-cDNAs suggest a regulatory role for NFI transcription factors in olfactory neuron gene expression.. Brain Res Mol Brain Res. 1999; 72(1).
- Fletcher CF, Jenkins NA, Copeland NG, Chaudhry AZ, Gronostajski RM. Exon structure of the nuclear factor I DNA-binding domain from C. elegans to mammals.. Mamm Genome. 1999; 10(4).
- Leahy P, Crawford DR, Grossman G, Gronostajski RM, Hanson RW. CREB binding protein coordinates the function of multiple transcription factors including nuclear factor I to regulate phosphoenolpyruvate carboxykinase (GTP) gene transcription.. J Biol Chem. 1999; 274(13).
- Chaudhry AZ, Vitullo AD, Gronostajski RM. Nuclear factor I-mediated repression of the mouse mammary tumor virus promoter is abrogated by the coactivators p300/CBP and SRC-1.. J Biol Chem. 1999; 274(11).
- Chaudhry AZ, Vitullo AD, Gronostajski RM. Nuclear factor I (NFI) isoforms differentially activate simple versus complex NFI-responsive promoters.. J Biol Chem. 1998; 273(29).
- Crawford DR, Leahy P, Hu CY, Chaudhry A, Gronostajski R, Grossman G, Woods J, Hakimi P, Roesler WJ, Hanson RW. Nuclear factor I regulates expression of the gene for phosphoenolpyruvate carboxykinase (GTP).. J Biol Chem. 1998; 273(22).
- Bandyopadhyay S, Starke DW, Mieyal JJ, Gronostajski RM. Thioltransferase (glutaredoxin) reactivates the DNA-binding activity of oxidation-inactivated nuclear factor I.. J Biol Chem. 1998; 273(1).
- Chaudhry AZ, Lyons GE, Gronostajski RM. Expression patterns of the four nuclear factor I genes during mouse embryogenesis indicate a potential role in development.. Dev Dyn. 1997; 208(3).
- Kulkarni S, Gronostajski RM. Altered expression of the developmentally regulated NFI gene family during phorbol ester-induced differentiation of human leukemic cells.. Cell Growth Differ. 1996; 7(4).
- Bandyopadhyay S, Gronostajski RM. Identification of a conserved oxidation-sensitive cysteine residue in the NFI family of DNA-binding proteins.. J Biol Chem. 1994; 269(47).
- Novak A, Goyal N, Gronostajski RM. Four conserved cysteine residues are required for the DNA binding activity of nuclear factor I.. J Biol Chem. 1992; 267(18).
- Knox JJ, Rebstein PJ, Manoukian A, Gronostajski RM. In vivo stimulation of a chimeric promoter by binding sites for nuclear factor I.. Mol Cell Biol. 1991; 11(6).
- Goyal N, Knox J, Gronostajski RM. Analysis of multiple forms of nuclear factor I in human and murine cell lines.. Mol Cell Biol. 1990; 10(3).
- Gronostajski RM, Knox J, Berry D, Miyamoto NG. Stimulation of transcription in vitro by binding sites for nuclear factor I.. Nucleic Acids Res. 1988; 16(5).
- Gronostajski RM. Site-specific DNA binding of nuclear factor I: effect of the spacer region.. Nucleic Acids Res. 1987; 15(14).
- Gronostajski RM. Analysis of nuclear factor I binding to DNA using degenerate oligonucleotides.. Nucleic Acids Res. 1986; 14(22).
- Gronostajski RM, Sadowski PD. The FLP recombinase of the Saccharomyces cerevisiae 2 microns plasmid attaches covalently to DNA via a phosphotyrosyl linkage.. Mol Cell Biol. 1985; 5(11).
- Gronostajski RM, Sadowski PD. The FLP protein of the 2-micron plasmid of yeast. Inter- and intramolecular reactions.. J Biol Chem. 1985; 260(22).
- Gronostajski RM, Sadowski PD. Determination of DNA sequences essential for FLP-mediated recombination by a novel method.. J Biol Chem. 1985; 260(22).
- Babineau D, Vetter D, Andrews BJ, Gronostajski RM, Proteau GA, Beatty LG, Sadowski PD. The FLP protein of the 2-micron plasmid of yeast. Purification of the protein from Escherichia coli cells expressing the cloned FLP gene.. J Biol Chem. 1985; 260(22).
- Gronostajski RM, Adhya S, Nagata K, Guggenheimer RA, Hurwitz J. Site-specific DNA binding of nuclear factor I: analyses of cellular binding sites.. Mol Cell Biol. 1985; 5(5).
- Gronostajski RM, Pardee AB, Goldberg AL. The ATP dependence of the degradation of short- and long-lived proteins in growing fibroblasts.. J Biol Chem. 1985; 260(6).
- Gronostajski RM, Goldberg AL, Pardee AB. The role of increased proteolysis in the atrophy and arrest of proliferation in serum-deprived fibroblasts.. J Cell Physiol. 1984; 121(1).
- Gronostajski RM, Field J, Hurwitz J. Purification of a primase activity associated with DNA polymerase alpha from HeLa cells.. J Biol Chem. 1984; 259(15).
- Field J, Gronostajski RM, Hurwitz J. Properties of the adenovirus DNA polymerase.. J Biol Chem. 1984; 259(15).
- Gronostajski RM, Nagata K, Hurwitz J. Isolation of human DNA sequences that bind to nuclear factor I, a host protein involved in adenovirus DNA replication.. Proc Natl Acad Sci U S A. 1984; 81(13).
- Gronostajski RM, Pardee AB. Protein degradation in 3T3 cells and tumorigenic transformed 3T3 cells.. J Cell Physiol. 1984; 119(1).
- Gronostajski RM, Goldberg AL, Pardee AB. Energy requirement for degradation of tumor-associated protein p53.. Mol Cell Biol. 1984; 4(3).
- Friefeld BR, Lichy JH, Field J, Gronostajski RM, Guggenheimer RA, Krevolin MD, Nagata K, Hurwitz J, Horwitz MS. The in vitro replication of adenovirus DNA.. Curr Top Microbiol Immunol. 1984; 110.
- Turner KJ, Gronostajski RM, Schmidt RR. Regulation of initial rate of induction of nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase during the cell cycle of synchronous Chlorella.. J Bacteriol. 1978; 134(3).
- Meredith MJ, Gronostajski RM, Schmidt RR, Gronostajski R. Physical and Kinetic Properties of the Nicotinamide Adenine Dinucleotide-specific Glutamate Dehydrogenase Purified from Chlorella sorokiniana. Plant Physiol. 1978; 61(6).
- Gronostajski RM, Yeung AT, Schmidt RR. Purification and properties of the inducible nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase from Chlorella sorokiniana.. J Bacteriol. 1978; 134(2).
- Israel DW, Gronostajski RM, Yeung AT, Schmidt RR. Regulation of accumulation and turnover of an inducible glutamate dehydrogenase in synchronous cultures of Chlorella.. J Bacteriol. 1977; 130(2).
Books and Book Chapters:
- Gronostajski R. Nuclear Factors. Encyc. Molec. Med.. 2002.
- Richard Gronostajski. Nuclear Factors. Encyclopedia of Molecular Medicine. 2002; 4.
- Richard Gronostajski. Nuclear Factor I. Encyclopedia of Molecular Medicine. 2002; 4.
- Israel, D. W., Gronostajski R. M., Yeung, A. T., Schmidt, R. R.. Regulation of glutamate dehydrogenase induction and turnover during the cell cycle of the eukaryote Chlorella.. Cell Cycle Regulation. 1978.
Professional Memberships:
- ISSCR; International Society for Stem Cell Research (2013–present)
- SDB; Society for Developmental Biology (2010–present)
- ASM; American Society for Microbiology (2003–2005)
- ASBMB; American Society for Biochemistry and Molecular BIology (1990–2004)
Presentations:
- "NFIB: A governor of epithelial-melanocyte stem cell behaviour in a shared niche" ISSCR, ISSCR (2013)
- "NFIX REGULATES THE FATE OF NEURAL STEM CELLS IN POSTNATAL MOUSE BRAIN" NYSTEM 2013, NYSTEM (2013)
- "Nuclear Factor I (NFI) transcription factors: a small family that does big things in brain development." Queensland Brain Institute, University of Queensland (2013)
- "Roles for Nuclear Factor I (NFI) transcription factors in lung, muscle, tooth and skin development." Institute for Molecular Bioscience, University of Queensland (2013)
- "Regenerative Medicine: A New Opportunity for Blood Center Diversification?" America's Blood Centers, America's Blood Centers (2012)
- "WNYSTEM: Western New York Stem Cell Culture and Analysis Center" NYSTEM Annual Meeting, NYSTEM (2012)
- "Multiple roles for Nuclear Factor I (NFI) transcription factors in the development of lung, brain, gut and other organ systems" Vanderbilt University, Vanderbilt University, Developmental Biology (2011)
- "Multiple roles for Nuclear Factor I (NFI) transcription factors in the development of brain, lung and other organ systems" NIH (2011)
- "Multiple roles for Nuclear Factor I (NFI) transcription factors in the development of brain, lung and other organ systems" Virginia Bioinformatics Institute, Virginia Tech (2011)
- "Multiple roles for the Nuclear Factor I (NFI) transcription factor family in metazoan development" NIH, NID (2003)
Service Activities:
- Jacobs School of Medicine & Biomedical Sciences Faculty Council; Member (2003–2009)
- Member Faculty Council, Jacobs School; Member (2018–present)
- Chair, Faculty Advisory Committee MRBSB, UB2020; Chair (2013–2015)
- Director: Genetics, Genomics & Bioinformatics Graduate Program; Director (2013–present)
- Member Faculty Practice Management Plan Governing Board; Member (2013–present)
- Director Western New York Stem Cell Culture and Analysis Center; Director (2011–present)
- Member Empire State Stem Cell Board (ESSCB); Member (2011–2013)
- Member Board of Directors UB Associates; Board of Directors (2009–2010)
- Standing Committee for Facilities Planning and Budget; Chair (2008–2009)
- Member Faculty Practice Management Plan Governing Board; Member (2008–2010)
- Member Faculty Advisory Committee UB2020 Molecular Recognition in Biological Systems/Bioinformatics Strategic Strength; Member (2005–2013)
- Founder Developmental Genomics Focus of Excellence; Director (2004–present)
- Member of Faculty Council 2003-2009; Member (2003–2009)
- Member Search Committee Bioinformatics; Committee Member (2003–2006)
- Chair of session in Frontiers of Bioinformatics 2003; Panel Member (2003)
- Member IGPBS steering committee; Member (2003–2007)
- Member Graduate Admissions committee; Member (2003–2007)
- Member IRCAF review panel 2003; Ad Hoc Reviewer (2003)
- Co-organizer of Biochemistry Annual Research Day; Organizing Committee Member (2003–2008)
- Member MSTP Admissions/Steering Committee 2003-present; Member (2003–present)
- Member IFR Committee 2001-present; Member (2001–present)