Core Facilities

Confocal Microscope and Flow Cytometry Facility

Wade Sigurdson, PhD, and Raymond Kelleher, PhD

Wade J. Sigurdson, PhD (left), has expertise on instrumentation including the Zeiss LSM-510 Meta NLO laser scanning confocal microscope. Raymond Kelleher, PhD, can assist you with flow cytometers such as the BD FACS Calibur system.

Our instrument laboratory provides training and consultation services for researchers whose work involves flow cytometry, confocal microscopy and gel-documentation.

Flow Cytometry (FACS) Instrumentation

We host analytical fluorescence-activated flow cytometers and a state-of-the-art high-speed cell sorter:

  • BD Fortessa SORP, four-laser, 14-color analyzer
  • BD FACS Calibur two-laser, four-color system
  • BD FACSAria three-laser, high-speed cell sorter capable of analyzing and selecting cells based on up to nine fluorescent colors
  • BD FACSArray Bioanalyzer that can perform multiplex analyses using the cytometric bead array technique
  • Zeiss K-Elispot automated imaging analysis system

We also have a network version of FCSExpress — an analytical software program — available for our visitors.

Microscopy Instrumentation

Our facility contains a Zeiss LSM-510 Meta NLO laser scanning confocal microscope attached to a Zeiss-inverted microscope. It is capable of simultaneous imaging of multiple-emission wavelengths, transmitted-light imaging and multiphoton capability through a Coherent Chameleon Ultra II laser.

A total of seven laser excitation lines (one tunable via the Chameleon laser), two single-wavelength emission channels and the multispectral “meta” detector allow a large number of dye combinations to be imaged in an individual specimen.

A microscope incubator and temperature controller is available for live-cell imaging.

Zeiss Axio Observer and Axio Imager wide-field fluorescent microscopes are available for bright field, DIC and phase contrast imaging, complete with cooled-CCD and color cameras.

These are also equipped with Zeiss’s Apotome structured-illumination system and deconvolution software for forming high-resolution optical slices of objects not well suited for confocal imaging. Image analysis and volume rendering software take the acquired datasets and perform 3-D reconstruction of cellular structures.

The Leica AS/LMD laser capture/laser microdissection system is available for isolation of single cells or small pieces of tissue from histological or live specimens.

Gel-Documentation and Ancillary Instrumentation

Visit our facility for:

  • Bio-Rad Doc Gel EZ imaging of ethidium bromide or SYBR green stained DNA gels and Coomassie Blue/Silver stained gels
  • Bio-Rad GS-700 flatbed scanning densitometer for film and gel scanning and flatbed scanning densitometry
  • Bio-Rad ChemiDoc MP electronic chemiluminescent and fluorescent imaging of Western blots
  • Bio-Rad multiplex real time PCR thermocyclers CFX-96 Touch and CFX-96 Connect
  • Nanodrop ND-1000 spectrophotometer
  • Bio-Tek Synergy absorbance, fluorescence and luminescence plate reader

Analysis Software for Images and Flow Cytometry

Researchers use our software to analyze data they acquire from the various instruments