Our instrument laboratory provides training and consultation services for researchers whose work involves flow cytometry, confocal microscopy and gel-documentation.
We host analytical fluorescence-activated flow cytometers and a state-of-the-art high-speed cell sorter:
We also have a network version of FCSExpress — an analytical software program — available for our visitors.
Our facility contains a Zeiss LSM-510 Meta NLO laser scanning confocal microscope attached to a Zeiss-inverted microscope. It is capable of simultaneous imaging of multiple-emission wavelengths, transmitted-light imaging and multiphoton capability through a Coherent Chameleon Ultra II laser.
A total of seven laser excitation lines (one tunable via the Chameleon laser), two single-wavelength emission channels and the multispectral “meta” detector allow a large number of dye combinations to be imaged in an individual specimen.
A microscope incubator and temperature controller is available for live-cell imaging.
Zeiss Axio Observer and Axio Imager wide-field fluorescent microscopes are available for bright field, DIC and phase contrast imaging, complete with cooled-CCD and color cameras.
These are also equipped with Zeiss’s Apotome structured-illumination system and deconvolution software for forming high-resolution optical slices of objects not well suited for confocal imaging. Image analysis and volume rendering software take the acquired datasets and perform 3-D reconstruction of cellular structures.
The Leica AS/LMD laser capture/laser microdissection system is available for isolation of single cells or small pieces of tissue from histological or live specimens.
Visit our facility for:
Researchers use our software to analyze data they acquire from the various instruments