This facility contains a Zeiss LSM-510 Meta NLO laser scanning confocal microscope attached to a Zeiss-inverted microscope. It is capable of simultaneous imaging of multiple-emission wavelengths, transmitted-light imaging and multiphoton capability through a Coherent Chameleon Ultra II laser.
A total of seven laser excitation lines (one tunable via the Chameleon laser), two single-wavelength emission channels and the multispectral “meta” detector allow a large number of dye combinations to be imaged in an individual specimen.
A microscope incubator and temperature controller is available for live-cell imaging.
Zeiss Axio Observer and Axioimager wide-field fluorescent microscopes are available for bright field, DIC and phase contrast imaging, complete with cooled-CCD and color cameras.
These are also equipped with Zeiss’s Apotome structured-illumination system and deconvolution software for forming high-resolution optical slices of objects not well suited for confocal imaging. Image analysis and volume rendering software take the acquired datasets and perform 3D reconstruction of cellular structures.
The Leica AS/LMD laser capture/laser micro-dissection system is available for isolation of single cells or small pieces of tissue from histological or live specimens.
406 Biomedical Research Building
Wade J. Sigurdson, PhD
Director
Confocal Microscopy Facility
406 Biomedical Research Building, University at Buffalo, Buffalo NY 14214
Phone: (716) 829-3589; Fax: (716) 829-3589
Email: wjs@buffalo.edu
Online reservation system requires a Calcium login. Contact
the facility for more information.
