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Core Facilities

Stem Cell Sequencing/Epigenomics Analysis

Yijun Sun, PhD, and Michael Buck, PhD.

Michael Buck, PhD (right), directs the Stem Cell Sequencing/Epigenomics Analysis facility and uses its resources to collaborate on studies with Yijun Sun, PhD, and other UB researchers.

We are a resource for epigenomic analysis of stem cells. Our facility functions interactively with other stem cell facilities at UB to characterize stem cells and their differentiated progeny.

The goals of this facility are to:

  • characterize the transcriptome and epigenome of all stem cell types and their derived lineages
  • provide experimental and computational support for investigator-initiated genomic expression or epigenetic experiments

This facility can determine:

  • gene expression and microRNA expression profiles using RNA sequencing
  • genomic DNA methylation using methylated DNA immunoprecipitation and sequencing
  • genome-wide histone modifications and transcription factor binding site localization using ChIP-seq
  • nucleosome positioning using gel-excised mono-nucleosomes and high-throughput DNA sequencing
  • chromatin accessibility by FAIRE-seq

Instrumentation

Our facility has instruments including:

  • illumina HiSeq 2500, a flexible and scalable platform that features two run modes, rapid run and high output run mode and the ability to process one or two flow cells simultaneously
  • illumina MiSeq, a platform specializing in more targeted sequencing geared toward smaller genomes and smaller search space investigation

Location

UB New York State Center of Excellence in Bioinformatics and Life Sciences
Room B3-123
Buffalo Niagara Medical Campus
701 Ellicott Street
Buffalo, NY 14203

Contact Information

Send general inquiries to cbi-ubnextgencore@buffalo.edu.

Director

Buck, Michael

Michael Buck, PhD

Associate Professor

701 Ellicott St, Center Of Excellence in Bioinformatics, Room B3-304 Buffalo, NY 14203

Phone: (716) 881-7569

Email: mjbuck@buffalo.edu

Laboratory Manager

No Picture

Sujith A. Valiyaparambil

Center of Excellence in Bioinformatics and Life Sciences Lab #B3-123

701 Ellicott Street, Buffalo, NY 14203

Phone: (716) 881-7514

Email: alphy@buffalo.edu