Departmental Research Facilities

The Cary Hall lab has an inverted microscope equipped with fluorescence and patch clamp capabilities. This configuration has a Nikon Diaphot-TMD inverted microscope equipped with epifluorescence, a Burleigh PCS-250 piezoelectric micromanipulator, an Adams & List patch clamp tower, all supported by a TMC 63-500 series vibration isolation table. 

Additionally, the setup is equipped with a Sutter Xenoworks micromanipulator and digital microinjector, Sutter P97 micropipette puller and a Narishige MF-900 microforge, and Perfusion system.

The voltage clamp setup includes an Axopatch 1D patch clamp. Data are acquired and digitized with a Molecular Dynamics Digidata 1322A and Pclamp 10.2.

Located at 132 Sherman Hall is a 400-square-foot lab/office which houses an electrophysiological setup.

This lab features:

• a vibration table
• Faraday cage
• Digidata D/A converter
• electrode puller
• computer dedicated to data collection and pClamp operation
  
• assorted general electrophysiological lab resources
• Dagan two electrode voltage clamp system driven by a P–Clamp 10.2 control system

In 133 Sherman Hall is 750 square feet equipped for general and molecular biochemistry.

This lab features:

• a sterile culture hood for preparation of human iPSCD cardiac myocytes and other cultured cells
• dissecting microscope
• Dell OptiPlex
• perfusion system
• vibratome
• stereo microscope
• shaking incubator
• PCR cycler
• centrifuges
• microfuge
• electrophoresis power supplies and chambers for DNA and RNA and protein electrophoresis and other small equipment items
• water baths
• Bio-Rad gel dryer thermal shaker
• Perkin Elmer Cetus DNA thermal cycler
• Forma CO2 incubator
• minus 80 C freezer
• minus 20 C freezer/fridge
• Mettler analytic balance
• pH meter

Included within the hospital is a 500-square-foot laboratory space equipped for molecular biochemistry.

• 45 Sherman Hall
• 132 Sherman Hall
• 133 Sherman Hall
• 201 Cary Hall
• Oishei Children's Hospital