Multispectral Imaging Suite

We provide training and consultation services for students, faculty, researchers and clinicians whose work involves digital whole slide imaging (WSI) and multispectral microscopy for research, teaching or clinical interests.

WSI is digitization of stained (IHC or IF) entire tissue specimen on a glass slide, resulting in a pyramid structure of multiple images at different resolutions where the baseline image has the highest resolution.

Please call us with questions about sample preparation to obtain optimal results from imaging.

• Leica DM500B Microscope - with Nuance EX Multispectral Imaging System
  • The system utilizes an optimized, high-throughput tunable filter that is electronically switchable to maximize either sensitivity or throughput at both visible and near-infrared wavelengths.
    
  • Nuance acquisition software incorporates easy to use tools for building custom spectral libraries, as well as powerful unmixing and analysis algorithms allowing spectral characterization and quantitation for each of the multi-labeled components in an image.
  • Spectral range of 420-720 nm for both Brightfield & Fluorescence.
• Leica Aperio VERSA whole slide imaging System
  • Capable of brightfield, fluorescence, & FISH imaging
    
  • Objectives include 5x, 10x, 20x, 40x oil & 63x oil immersion
  • Scan slides from 0.9mm to 1.2mm thick
  • Z-stack capture for thick samples
• Aperio ScanScope CS System
  • Capable of scanning at 20x Brightfield with a single high-resolution 20x/0.75 objective lens.
    
  • A 2x magnification changer enables scanning at 40x.
• ImageScope Software
  • Available free for download from Leica
•  Annotation Workstation 
  • Cintiq 22HD Creative Pen Display for precision digital annotations
  • Image Pro 3D software
• Pacific Image PowerSlide 5000 CCD Slides Scanner
  • Converts large numbers of 35mm mounted slides into digital files that can be searched, processed, printed, or delivered electronically.
  • It has an optical resolution of 5000dpi and 48-bit data conversion.
• NX12 Atomic Force Microscope (AFM)
  
  • Additionally the core assists Dr. Yongho Bae’s lab with their NX12 AFM (Park Systems) mounted on a Nikon Eclipse Ti2 inverted microscope.
  • It has the ability to obtain high-quality images at nanoscale resolution and measure mechanical properties (elasticity, stiffness, and adhesion force) of cells, tissues, and biomaterials.
  • The decoupled/flexure scanner design makes it a more accurate system for mechanical measurements and prevents bowing effect while imaging samples resulting in clean images without any background curvature.
  • Contact Dr. Bae for potential collaborative use.

Please acknowledge facility use in grants and publications.

If you are publishing data acquired or processed using MSI Lab instruments, please acknowledge us in your papers by mentioning where you did your imaging, sample preparation, or image analysis. This simple step supports our mission to help you with your research. If a specific staff member helped you above and beyond what is expected, thank them, by name.

You can use a format similar to the one suggested below.

"We acknowledge the assistance of the Multispectral Imaging Suite in the Dept. of Pathology & Anatomical Sciences, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo."

Finally, if you think that the Core Lab’s support of your research may be sufficient to merit authorship, take a look at  authorship guidelines and email us to discuss this further. As would be the case with any prospective author of a manuscript, do NOT include any of the core lab staff as authors on a paper without proper consultation prior to submission.

Citations (with or without acknowledgements) of all papers generated using Core lab resourses would be gratefully appreciated. This kind of documentation of the research that depends on us is very important when it comes to justification for purchasing new or upgraded equipment.

Pricing for resource services is based on the actual cost of performing the services, set by an internal review of the time and consumables used for the services.

Jacobs School of Medicine and Biomedical Sciences
955 Main Street, Room 3125
Buffalo, NY 14203-1121