John K. Crane MD, PhD

John Crane

John K. Crane

Professor of Medicine; Adjunct Professor of Micro & Immunol. and of Pharmacol & Tox.

Department of Medicine

Jacobs School of Medicine & Biomedical Sciences

Specialty/Research Focus

Bacterial Pathogenesis; DNA Replication, Recombination and Repair; Infectious Disease; Infectious Diseases; Medical Microbiology; Microbial Pathogenesis; Research Lab; Vitamins and Trace Nutrients

Contact Information
317 Biomedical Research Building
Buffalo, New York 14215
Phone: (716) 829-2676
Fax: (716) 829-3889

Patient Care

This faculty member is affiliated with UBMD, practicing physicians who are also professors in the Jacobs School.

View this physician on UBMD

Professional Summary:

I care for patients who are hospitalized at Erie County Medical Center where I also serve as the hospital epidemiologist addressing infection control.

I teach medical students, residents, and fellows in both hospital and classroom settings. In UB’s schools of medicine and dentistry, I teach a variety of topics including microbiology, pharmacology and toxicology, oral biology, and gastrointestinal systems, host defenses, and global health.

I also conduct laboratory research on diarrhea-producing strains of E. coli bacteria. My lab focuses on enteropathogenic Escherichia coli (EPEC), Shiga-toxigenic E. coli (STEC, aka EHEC) and enterotoxigenic E. coli (ETEC). We are working on the role of intestinal host defenses such as nitric oxide and on the immune modulatory effects of adenosine. We have discovered that zinc can directly inhibit the virulence of pathogenic bacteria, and we are working on turning these laboratory findings into treatments. In our work on zinc we collaborate with Michael Duffey, PhD, in the Department of Physiology and Biophysics.

Recently we have discovered that zinc can inhibit the development of resistance to antibiotics in Escherichia coli and other bacteria. Zinc does this by its ability to inhibit the SOS response, a bacterial stress response triggered by damage to the bacterial DNA. We are collaborating with Dr. Mark Sutton of Biochemistry to better determine the mechanism of zinc in this regard.

I am interested in international medicine and global health and participate in an annual medical mission trip to Honduras, a trip in which student volunteers are encouraged to participate. Closer to home, I am a volunteer physician at Good Neighbors Health Center, a free clinic for the underserved on Jefferson Avenue in Buffalo. Resident physicians are encouraged to volunteer, and students may also be able to arrange clinical experiences.

I am Co-Medical Director, with Dr. Ryosuke Osawa, of the Erie County TB Clinic.

Learning experiences in my laboratory, in infection prevention and hospital epidemiology, or in international health, may be available for motivated students, residents, and fellows.

Education and Training:

  • Certificate, Denver TB Course, National Jewish Health (2015)
  • Fellowship, Infectious Diseases, University of Virginia (1990)
  • Residency, Medicine, University of Rochester, Strong Memorial Hospital (1987)
  • MD, MD, University of Virginia (1984)
  • PhD, Pharmacology, University of Virginia (1983)


  • Medical Director, Erie County Health Dept., Erie County Tuberculosis Clinic (2015-present)
  • Professor, Medicine, Jacobs School of Medicine & Biomedical Sciences (2014-present)
  • Associate Professor, Medicine, University at Buffalo Jacobs School of Medicine & Biomedical Sciences (1994-present)

Awards and Honors:

  • Neighborhood Steward, Little Free Library (2024)
  • "Top Doctor Award" (2023)
  • Outstanding Physician Award, ECMC, 2018 (2018)
  • Mentor for Recipient of IDSA Student Scholar Award, 2011 (2011)
  • Fellow of the Infectious Diseases Society of America (2009)
  • American Society for Microbiology Service Award (2008)
  • NYS Hospital Acquired Infections Technical Advisory Group

Research Expertise:

  • Intestinal pathogenic Escherichia coli pathogenesis: Diarrhea-producing E. coli
  • Mechanism of Emergence of Antibiotic Resistance: Role of SOS Response in Antibiotic Resistance
  • Microbial pathogenesis
  • Role of nitric oxide in host defense in the intestinal tract: Role of nitric oxide in host defense in intestine
  • Role of zinc in intestinal infection

Research Centers:

  • Witebsky Center for Microbial Pathogenesis and Immunology

UB 2020 Strategic Strengths:

  • Molecular Recognition in Biological Systems and Bioinformatics

Grants and Sponsored Research:

  • May 2020–December 2022
    A study of various antibiotics against Cutibacterium acnes and Staphylococcus epidermidis in permanent and dissolvable cement beads.
    American Shoulder and Elbow Surgeons
    Role: Co-Principal Investigator
  • June 2019–June 2021
    SOS Response as a Generator of Antibiotic Resistance and Role of Zinc as SOS Inhibitor
    National Institutes of Health
    Role: Principal Investigator
  • December 2014–December 2015
    Contamination of Water and the Environment in a Peri-Urban Neighborhood in Tegucigalpa, Honduras
    University at Buffalo
    Role: Principal Investigator
  • July 2012–June 2014
    Enhancing Host Defenses against Enteropathogenic and Shiga toxigenic E. coli
    Role: Principal Investigator
  • May 2010–May 2013
    Effect of Zinc in Attaching-and-Effacing E. coli infections
    Role: Co-Principal Investigator
  • September 2003–January 2006
    Host cell killing by EPEC: central role in pathogenesis
    Role: Principal Investigator
  • September 2003–August 2005
    Enteropathogenic and toxigenic E. coli Interactions
    Role: Principal Investigator


  • Technology New Disclosure 7260 Technology New Disclosure 7260, July , 2018.

Journal Articles:

See all (46 more)

Professional Memberships:

  • American Society for Microbiology, WNY Branch; past president, Member Executive Committee (2022–present)
  • American Society for Microbiology, National (2001–present)
  • Infectious Diseases Society of America; Fellow of the IDSA Member since 1989 (1989–present)


  • "CME Seminars, Lectures on ID Topics" CME Seminars, Dr. Rick Scott, Medical Director (2024)
  • "4 Lectures on ID Topics" CME Seminars, dba as American Medical Seminars (2023)
  • "Covid-19: Lessons Learned and Looking Forward" American Association of Nurse Practitioners NPA Teaching Day (2022)
  • "From Plaque to Peril: An Aggregation of Risk" Western NY ASM Spring Meeting, in person (2022)
  • "Update on Covid-19" 2022 Physician Assistants for Global Health (PAGH) Virtual conference. (2022)
  • "Medical Grand Rounds, on Tuberculosis" Medical Grand Rounds, Catholic Health System, Buffalo Mercy Hospital. Nov., 2019 (2021)
  • "A difficult TB Case" Infectious Diseases Research Conference; Jacobs School of Medicine & Biomedical Sciences (2020)
  • "Infectious Complications of the Opioid Addiction Crisis" American Medical Seminars; AMS (2020)
  • "Zinc Inhibits Hypermutation by Blocking the SOS Response in E. coli" Amer. Soc. for Microbial. Microbe 2016 Meeting; ASM (2016)
  • "Antimicrobial Stewardship" WNY APIC Chapter Spring Meeting; Assoc. for Professionals in Infection Control (2016)
  • "Infections due to Drug Resistant Organisms" Continuing Education Conference for Health Now; Health Now, formerly Exigence, a physician hospitalist staffing group (2015)
  • "Point-of-use Microbiology for Water, Sanitation, and the Environment in Honduras" Spring meeting, WNY ASM; Western NY Brach of American Soc. for Microbiology (2015)
  • "Skin and Soft Tissue Infections" Primary Care Update, CME Conference; UB Dept. of Medicine (2015)
  • "Legionnaire's Disease" 2013 Fall Symposium; Western NY Branch, American Society for Microbiology (2013)
  • "Adenosine and Purinergic Signaling in Enteropathogenic and Shiga toxigneic E. coli infection" Infectious Diseases Research Conference; University of New Mexico; Infectious Diseases Division (2013)
  • "Efecto de zinc en Infección con E. coli Enteropatogénica y Shiga-toxigénica" Research Day; Universidad Nacional Autónoma de Honduras; Microbiology Dept. (2012)
  • "Effect of Zinc on Enteropathogenic and Shiga-toxigneic E. coli" Experimental Biology; American Society for Nutrition; American Society for Nutrition Symposium (2011)
See all (7 more)

Service Activities:

  • UB Heals Street Ministry; Physician Mentor, UB Heals (2022–2023)
  • Volunteer Vaccinator with Evergreen Clinic; Volunteer Vaccinator, Monkeypox Outbreak, summer, 2022 (2022)
  • Interviewer for Residency Program; Resident’s Research Day/ Celebration of Scholarship. (2022)
  • Buffalo Niagara "Burn-Out Committee," chaired by Dr. Howard Faden (2022–present)
  • Dean's COVID Research Integration Work Group; Member (2021–2022)
  • Worked for 2 days as a volunteer Covid vaccinator with ECDOH, at Bowmansville Fire Dept. and at ECC South Campus.; Volunteer Physician Vaccinator, Erie County Health Dept. (2021)
  • UB Profile does not appear to acknowledge or recognize interviews or appearances on news media, such as TV interviews on WIVB, for Buffalo News, the Batavian (newspaper), etc.; Interviews to members of the news media about Covid-19, vaccines, vaccine hesitancy, ec. (2020–2022)
  • WNY COVID-19 Research Collaborative Executive Committee; Executive Committee (2020–2021)
  • Zoom Conferences with churches in Buffalo about Covid-19, including with Unity Baptist Church (Shadevia Marshall), Macedonian Baptist church (Pastor Julian Cook), St. Paul's Lutheran Church (Pastor Adam Miller), also with CMDA and as a panelist with Dr. Leeland Jones.; Presenter to Churches, mostly in Minority Communities, about Covid-19 and Covid-19 Vaccines. (2020–2021)
  • NIH; F13 Fellowship Grant; Dr. Tamara McNealy, SRO;; Grant Reviewer (2018)
  • NIAID; ZAI1 JBS; Reviewer;; Grant Reviewer (2016)
  • City-Wide Antibiotic Committee; Member (2016–present)
  • NIAID; Systems Biology of Antibiotic Resistance;; Grant Reviewer (2015)
  • NIH; ZRG IDM-T (02) M;; Ad Hoc Reviewer (2014)
  • STOP Sepsis Committee, ECMC Member of the Committee charged with responding to a new New York State law passed in July, 2013, requiring all hospitals to have a comprehensive plan to recognize and respond to patients with sepsis.; Member (2013–2014)
  • Gastrointestinal Mucosal Pathobiology (GMPB); GMPB Study Section;; Ad Hoc Reviewer (2012–2013)
  • Unqualified Promotions; Member (2011–2015)
  • New York State Hosp. Acquired Infections Technical Advisory Working Group; Member (2010–present)
  • Frontiers in Cellular and Infection Microbiology; Reviewer (2010–present)
  • Volunteer Physician, Good Neighbors Health Center Good Neighbors Health Center is a free clinic for the uninsured on Buffalo's East side, 175 Jefferson Avenue. The Monday Night clinic is On hold for now due to Covid-19.; Volunteer (2009–2020)
  • Antimicrobial Stewardship Committee, ECMC Hospital; Committee Member (2004–2020)
  • NIH; Special Emphasis Panel on Biodefense; Reviewed a Program Project Grant;; Grant Reviewer (2003)

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Contact Information

317 Biomedical Research Building
Buffalo, New York 14215
Phone: (716) 829-2676
Fax: (716) 829-3889

This faculty member is affiliated with UBMD, practicing physicians who are also professors in the Jacobs School.

View this physician on UBMD