This course is designed to provide the student with an understanding of the principles and practice of the large-scale expression, purification, and characterization of proteins for biochemical, biophysical, and structural analyses. Topics covered include: cloning; expression in bacteria, yeast, and insect cell systems; theoretical and practical applications of protein purification; detergents and membrane proteins; maintenance of sample activity using buffers, additives, and inhibitors; SDS-PAGE and western-blot analysis; small-angle X-ray scattering; and crystallization.